SDS3 (SUDS3) (NM_022491) Human Tagged ORF Clone

CAT#: RC210535

SUDS3 (Myc-DDK-tagged)-Human suppressor of defective silencing 3 homolog (S. cerevisiae) (SUDS3)

Product Images

Circular map for RC210535

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  Western blot validation of overexpression lysate (Cat# LY402929) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210535 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified SUDS3 protein (Cat# TP310535). The protein was produced from HEK293T cells transfected with SUDS3 cDNA clone (Cat# RC210535) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SDS3
• Synonyms: SAP45; SDS3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210535 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGCCGCGGGGCTGCTGGCCCCGGCCCCGGCCCAGGCTGGAGCGCCGCCGGCCCCCGAGTACTACC
  CCGAGGAGGATGAAGAGCTGGAGAGCGCCGAGGACGACGAGCGCAGCTGTCGGGGCCGCGAGTCGGACGA
  AGACACTGAGGATGCTAGTGAAACTGACCTGGCAAAGCATGATGAAGAAGACTATGTAGAAATGAAGGAA
  CAGATGTATCAGGACAAACTGGCTTCTCTCAAGAGGCAGTTGCAACAACTGCAAGAAGGTACATTACAGG
  AATATCAGAAGAGAATGAAAAAACTAGATCAGCAGTACAAAGAGAGGATACGGAATGCAGAACTCTTCCT
  CCAGCTGGAAACTGAACAAGTGGAACGAAATTACATTAAAGAAAAGAAGGCAGCAGTGAAAGAATTTGAA
  GACAAGAAGGTTGAGCTGAAAGAGAACCTGATTGCTGAGCTAGAAGAAAAGAAGAAAATGATTGAAAATG
  AAAAGCTGACAATGGAACTGACTGGAGATTCTATGGAGGTGAAACCTATCATGACCAGAAAGTTGCGGAG
  GCGACCAAATGATCCCGTCCCCATCCCAGACAAGAGGAGGAAACCTGCTCCAGCCCAGCTAAACTATTTG
  TTAACAGATGAACAGATCATGGAGGATCTGAGAACATTAAATAAGCTTAAGTCACCCAAGAGACCAGCAT
  CTCCATCCTCTCCTGAGCACTTGCCTGCAACACCCGCGGAATCTCCAGCCCAGAGGTTCGAAGCTCGGAT
  AGAAGATGGCAAACTGTACTATGACAAAAGATGGTACCACAAGAGCCAGGCCATCTATCTGGAGTCAAAG
  GACAACCAGAAACTGAGCTGCGTGATCAGTTCTGTAGGAGCCAATGAGATCTGGGTGAGGAAGACAAGTG
  ACAGCACCAAGATGAGGATCTACCTGGGCCAGCTTCAGCGCGGGCTCTTCGTGATCCGCCGGCGCTCAGC
  TGCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210535 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSAAGLLAPAPAQAGAPPAPEYYPEEDEELESAEDDERSCRGRESDEDTEDASETDLAKHDEEDYVEMKE
  QMYQDKLASLKRQLQQLQEGTLQEYQKRMKKLDQQYKERIRNAELFLQLETEQVERNYIKEKKAAVKEFE
  DKKVELKENLIAELEEKKKMIENEKLTMELTGDSMEVKPIMTRKLRRRPNDPVPIPDKRRKPAPAQLNYL
  LTDEQIMEDLRTLNKLKSPKRPASPSSPEHLPATPAESPAQRFEARIEDGKLYYDKRWYHKSQAIYLESK
  DNQKLSCVISSVGANEIWVRKTSDSTKMRIYLGQLQRGLFVIRRRSAA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022491
• ORF Size: 984 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022491.3
• RefSeq Size: 4728 bp
• RefSeq ORF: 987 bp
• Locus ID: 64426
• UniProt ID: Q9H7L9
• Cytogenetics: 12q24.23
• Protein Families: Transcription Factors
• MW: 38.1 kDa
• Gene Summary: SDS3 is a subunit of the histone deacetylase (see HDAC1; MIM 601241)-dependent SIN3A (MIM 607776) corepressor complex (Fleischer et al., 2003 [PubMed 12724404]).[supplied by OMIM, Mar 2008]