MAN2B2 (NM_015274) Human Tagged ORF Clone

CAT#: RC210523

MAN2B2 (Myc-DDK-tagged)-Human mannosidase, alpha, class 2B, member 2 (MAN2B2)

Product Images

Circular map for RC210523

• 

  Western blot validation of overexpression lysate (Cat# LY414664) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210523 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified MAN2B2 protein (Cat# TP310523). The protein was produced from HEK293T cells transfected with MAN2B2 cDNA clone (Cat# RC210523) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MAN2B2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210523 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCAGCTGTGCTGGCTGCCGCTGCTGGCACCGCTCCTGTTGCTGCGACCGCCAGGGGTCCAGTCCG
  CCGGCCCCATCCGGGCCTTCGTGGTGCCCCACAGCCACATGGACGTGGGCTGGGTCTACACTGTGCAGGA
  AAGCATGCGGGCGTACGCCGCCAATGTCTACACCTCAGTGGTGGAAGAGCTGGCCCGCGGCCAGCAGCGC
  CGGTTCATCGCTGTGGAGCAGGAGTTTTTCCGGCTGTGGTGGGATGGCGTCGCCTCGGACCAGCAGAAAT
  ACCAGGTCCGCCAGCTCCTGGAGGAAGGACGCCTGGAATTTGTCATCGGAGGCCAGGTCATGCATGACGA
  GGCTGTGACGCACCTTGATGACCAGATCCTGCAGCTCACAGAAGGACACGGGTTTCTCTATGAAACATTT
  GGGATCCGGCCACAGTTCTCCTGGCACGTTGACCCGTTTGGCGCCTCTGCCACGACGCCCACCCTATTTG
  CGCTGGCGGGCTTCAATGCCCACCTCGGCTCCCGGATCGACTACGACCTGAAGGCAGCCATGCAGGAGGC
  CCGGGGGCTGCAGTTCGTGTGGCGAGGGTCCCCATCCCTCTCAGAGCGGCAGGAAATCTTCACGCACATC
  ATGGACCAGTACAGCTACTGCACCCCGTCCCACATCCCTTTCTCCAACAGGTCAGGATTTTACTGGAATG
  GCGTGGCTGTCTTCCCCAAGCCTCCCCCAGATGGGGTGTACCCCAACATGAGTGAGCCTGTCACCCCAGC
  CAACATCAACCTCTATGCCGAGGCCCTGGTGGCCAACGTGAAGCAGAGGGCCGCCTGGTTCCGGACACCG
  CACGTCCTCTGGCCCTGGGGATGTGACAAGCAGTTCTTCAATGCCTCGGTGCAGTTTGCCAACATGGACC
  CGCTGCTGGACCACATCAACAGCCATGCTGCCGAGCTCGGTGTCTCGGTGCAGTATGCCACGCTGGGCGA
  CTACTTCCGTGCCCTGCACGCTCTCAATGTCACCTGGCGTGTCCGCGACCACCACGACTTCCTGCCCTAT
  TCCACAGAACCATTCCAGGCCTGGACGGGCTTCTACACGTCCCGCAGCTCACTGAAGGGGCTGGCCCGGC
  GAGCCAGCGCCTTGTTGTATGCCGGGGAGTCCATGTTCACACGCTACCTGTGGCCGGCCCCCCGTGGGCA
  TCTGGACCCCACCTGGGCCCTGCAGCAGCTCCAGCAGCTTCGCTGGGCCGTCTCCGAGGTCCAGCACCAT
  GATGCCATCACTGGGACTGAGTCCCCCAAGGTGAGAGACATGTACGCAACGCACCTGGCCTCGGGGATGC
  TGGGCGTGCGCAAGCTGATGGCCTCCATCGTCCTAGATGAGCTCCAGCCCCAGGCACCCATGGCGGCCAG
  CTCCGATGCAGGACCTGCAGGACATTTTGCCTCGGTCTACAACCCGCTGGCCTGGACGGTCACCACCATC
  GTCACCCTGACTGTTGGTTTCCCTGGAGTCCGCGTCACAGATGAGGCGGGCCACCCAGTGCCCTCGCAGA
  TCCAGAACTCAACAGAGACCCCATCTGCGTATGACCTGCTTATTCTGACCACAATCCCAGGCCTCAGTTA
  CCGGCACTACAGCATCAGACCCACTGCAGGGGCCCAAGAGGGCACCCAGGAGCCGGCTGCCACTGTGGCG
  AGCACCCTTCAATTTGGCCGCAGGCTGAGGAGACGCACCAGCCATGCGGGCAGGTACTTGGTGCCTGTGG
  CAAACGACTGCTACATTGTGCTGCTCGACCAGGATACCAACCTGATGCACAGCATCTGGGAGAGACAGAG
  TAACCGAACGGTGCGCGTGACCCAGGAATTCCTGGAGTACCACGTCAACAGGGATGTGAAACAGGGCCCC
  ATTTCCGATAACTACCTGTTCACACCGGGCAAGGCCGCGGTGCCTGCGTGGGAAGCTGTGGAAATGGAGA
  TTGTGGCGGGACAGCTTGTGACTGAGATCCGGCAGTACTTCTACAGGAACATGACAGCACAGAATTACAC
  GTATGCAATCCGCTCCCGGCTCACCCATGTGCCGCAGGGCCATGACGGGGAGCTGCTCTGCCACCGGATA
  GAGCAGGAGTACCAAGCCGGCCCCCTGGAGCTGAACCGTGAGGCTGTCCTGAGGACCAGCACCAACCTAA
  ACAGCCAGCAGGTCATCTACTCAGACAACAACGGCTACCAGATGCAGCGGAGGCCCTACGTTTCCTATGT
  GAACAACAGCATCGCCCGGAATTACTACCCCATGGTTCAGTCGGCCTTCATGGAGGATGGCAAAAGCAGG
  CTTGTGTTGCTGTCGGAGCGGGCACATGGCATCTCCAGCCAAGGGAATGGGCAGGTGGAGGTCATGCTCC
  ACCGGCGGCTGTGGAACAACTTCGACTGGGACCTGGGCTACAACCTCACGCTGAACGACACCTCAGTCGT
  CCACCCAGTGCTCTGGCTTCTGCTGGGATCCTGGTCCCTCACCACTGCCCTGCGCCAGAGGAGCGCACTG
  GCGCTGCAGCACAGGCCCGTGGTGCTGTTCGGAGACCTCGCTGGGACTGCGCCGAAGCTCCCAGGACCCC
  AGCAGCAAGAGGCCGTGACGCTGCCCCCGAATCTTCACCTGCAGATCCTGAGCATCCCTGGCTGGCGCTA
  CAGCTCCAACCACACGGAGCACTCTCAGAATCTCCGGAAAGGCCATCGAGGGGAAGCCCAGGCTGACCTC
  CGCCGTGTCCTGCTGCGGCTCTACCACCTGTATGAAGTGGGCGAGGACCCAGTCCTGTCTCAGCCAGTAA
  CAGTGAATCTGGAGGCTGTGCTGCAGGCGCTGGGGTCCGTGGTGGCAGTGGAGGAGCGCTCGCTCACAGG
  GACCTGGGATTTGAGCATGCTGCACCGCTGGAGCTGGAGGACGGGGCCTGGCCGCCACAGAGGTGACACC
  ACCTCTCCCTCGAGGCCACCAGGAGGCCCCATCATCACCGTCCACCCAAAGGAAATCCGGACGTTCTTTA
  TTCACTTTCAACAGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210523 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGQLCWLPLLAPLLLLRPPGVQSAGPIRAFVVPHSHMDVGWVYTVQESMRAYAANVYTSVVEELARGQQR
  RFIAVEQEFFRLWWDGVASDQQKYQVRQLLEEGRLEFVIGGQVMHDEAVTHLDDQILQLTEGHGFLYETF
  GIRPQFSWHVDPFGASATTPTLFALAGFNAHLGSRIDYDLKAAMQEARGLQFVWRGSPSLSERQEIFTHI
  MDQYSYCTPSHIPFSNRSGFYWNGVAVFPKPPPDGVYPNMSEPVTPANINLYAEALVANVKQRAAWFRTP
  HVLWPWGCDKQFFNASVQFANMDPLLDHINSHAAELGVSVQYATLGDYFRALHALNVTWRVRDHHDFLPY
  STEPFQAWTGFYTSRSSLKGLARRASALLYAGESMFTRYLWPAPRGHLDPTWALQQLQQLRWAVSEVQHH
  DAITGTESPKVRDMYATHLASGMLGVRKLMASIVLDELQPQAPMAASSDAGPAGHFASVYNPLAWTVTTI
  VTLTVGFPGVRVTDEAGHPVPSQIQNSTETPSAYDLLILTTIPGLSYRHYSIRPTAGAQEGTQEPAATVA
  STLQFGRRLRRRTSHAGRYLVPVANDCYIVLLDQDTNLMHSIWERQSNRTVRVTQEFLEYHVNRDVKQGP
  ISDNYLFTPGKAAVPAWEAVEMEIVAGQLVTEIRQYFYRNMTAQNYTYAIRSRLTHVPQGHDGELLCHRI
  EQEYQAGPLELNREAVLRTSTNLNSQQVIYSDNNGYQMQRRPYVSYVNNSIARNYYPMVQSAFMEDGKSR
  LVLLSERAHGISSQGNGQVEVMLHRRLWNNFDWDLGYNLTLNDTSVVHPVLWLLLGSWSLTTALRQRSAL
  ALQHRPVVLFGDLAGTAPKLPGPQQQEAVTLPPNLHLQILSIPGWRYSSNHTEHSQNLRKGHRGEAQADL
  RRVLLRLYHLYEVGEDPVLSQPVTVNLEAVLQALGSVVAVEERSLTGTWDLSMLHRWSWRTGPGRHRGDT
  TSPSRPPGGPIITVHPKEIRTFFIHFQQQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015274
• ORF Size: 3027 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015274.3
• RefSeq Size: 4201 bp
• RefSeq ORF: 3030 bp
• Locus ID: 23324
• UniProt ID: Q9Y2E5
• Cytogenetics: 4p16.1
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Other glycan degradation
• MW: 114 kDa