GPR64 (ADGRG2) (NM_001079859) Human Tagged ORF Clone

CAT#: RC210484

GPR64 (Myc-DDK-tagged)-Human G protein-coupled receptor 64 (GPR64), transcript variant 2

Product Images

Circular map for RC210484

• 

  Western blot validation of overexpression lysate (Cat# LY421561) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210484 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GPR64
• Synonyms: CBAVDX; EDDM6; GPR64; HE6; TM7LN2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210484 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTTTCTCTGTCAGGCAGTGTGGCCATGTTGGCAGAACTGAAGAAGTTTTACTGACGTTCAAGATAT
  TCCTTGTCATCATTTGTCTTCATGTCGTTCTGGTAACATCCCTGGAAGAAGATACTGATAATTCCAGTTT
  GTCACCACCACCTGCTAAATTATCTGTTGTCAGTTTTGCCCCCTCCTCCAATGGTACTCCAGAGGTTGAA
  ACAACAAGCCTCAATGATGTTACTTTAAGCTTACTCCCTTCAAACGAAACAGGCGTCAAACCCCAGAGAA
  ATATCTGCAATTTGTCATCTATTTGCAATGACTCAGCATTTTTTAGAGGTGAGATCATGTTTCAATATGA
  TAAAGAAAGCACTGTTCCCCAGAATCAACATATAACGAATGGCACCTTAACTGGAGTCCTGTCTCTAAGT
  GAATTAAAACGCTCAGAGCTCAACAAAACCCTGCAAACCCTAAGTGAGACTTACTTTATAATGTGTGCTA
  CAGCAGAGGCCCAAAGCACATTAAATTGTACATTCACAATAAAACTGAATAATACAATGAATGCATGTGC
  TGTAATAGCTGCTTTGGAAAGAGTAAAGATTCGACCAATGGAACACTGCTGCTGTTCTGTCAGGATACCC
  TGCCCTTCCTCCCCAGAAGAGTTGGAAAAGCTTCAGTGTGACCTGCAGGATCCCATTGTCTGTCTTGCTG
  ACCATCCACGTGGCCCACCATTTTCTTCCAGCCAATCCATCCCAGTGGTGCCTCGGGCCACTGTGCTTTC
  CCAGGTCCCCAAAGCTACCTCTTTTGCTGAGCCTCCAGATTATTCACCTGTGACCCACAATGTTCCCTCT
  CCAATAGGGGAGATTCAACCCCTTTCACCCCAGCCTTCAGCTCCCATAGCTTCCAGCCCTGCCATTGACA
  TGCCCCCACAGTCTGAAACGATCTCTTCCCCTATGCCCCAAACCCATGTCTCCGGCACCCCACCTCCTGT
  GAAAGCCTCATTTTCCTCTCCCACCGTGTCTGCCCCTGCGAATGTCAACACTACCAGCGCACCTCCTGTC
  CAGACAGACATCGTCAACACCAGCAGTATTTCTGATCTTGAGAACCAAGTGTTGCAGATGGAGAAGGCTC
  TGTCCTTGGGCAGCCTGGAGCCTAACCTCGCAGGAGAAATGATCAACCAAGTCAGCAGACTCCTTCATTC
  CCCGCCTGACATGCTGGCCCCTCTGGCTCAAAGATTGCTGAAAGTAGTGGATGACATCGTCCTACAGCTG
  AACTTTTCAAACACGACTATAAGTCTAACCTCCCCTTCTTTGGCTCTGGCTGTGATCAGAGTGAATGCCA
  GTAGTTTCAACACAACTACCTTTGTGGCCCAAGACCCTGCAAATCTTCAGGTTTCTCTGGAAACCCAAGC
  TCCTGAGAACAGTATTGGCACAATTACTCTTCCTTCATCGCTGATGAATAATTTACCAGCTCATGACATG
  GAGCTAGCTTCCAGGGTTCAGTTCAATTTTTTTGAAACACCTGCTTTGTTTCAGGATCCTTCCCTGGAGA
  ACCTCTCTCTGATCAGCTACGTCATATCATCGAGTGTTGCAAACCTGACCGTCAGGAACTTGACAAGAAA
  CGTGACAGTCACATTAAAGCACATCAACCCGAGCCAGGATGAGTTAACAGTGAGATGTGTATTTTGGGAC
  TTGGGCAGAAATGGTGGCAGAGGAGGCTGGTCAGACAATGGCTGCTCTGTCAAAGACAGGAGATTGAATG
  AAACCATCTGTACCTGTAGCCATCTAACAAGCTTCGGCGTTCTGCTGGACCTATCTAGGACATCTGTGCT
  GCCTGCTCAAATGATGGCTCTGACGTTCATTACATATATTGGTTGTGGGCTTTCATCAATTTTTCTGTCA
  GTGACTCTTGTAACCTACATAGCTTTTGAAAAGATCCGGAGGGATTACCCTTCCAAAATCCTCATCCAGC
  TGTGTGCTGCTCTGCTTCTGCTGAACCTGGTCTTCCTCCTGGACTCGTGGATTGCTCTGTATAAGATGCA
  AGGCCTCTGCATCTCAGTGGCTGTATTTCTTCATTATTTTCTCTTGGTCTCATTCACATGGATGGGCCTA
  GAAGCATTCCATATGTACCTGGCCCTTGTCAAAGTATTTAATACTTACATCCGAAAATACATCCTTAAAT
  TCTGCATTGTCGGTTGGGGGGTACCAGCTGTGGTTGTGACCATCATCCTGACTATATCCCCAGATAACTA
  TGGGCTTGGATCCTATGGGAAATTCCCCAATGGTTCACCGGATGACTTCTGCTGGATCAACAACAATGCA
  GTATTCTACATTACGGTGGTGGGATATTTCTGTGTGATATTTTTGCTGAACGTCAGCATGTTCATTGTGG
  TCCTGGTTCAGCTCTGTCGAATTAAAAAGAAGAAGCAACTGGGAGCCCAGCGAAAAACCAGTATTCAAGA
  CCTCAGGAGTATCGCTGGCCTTACATTTTTACTGGGAATAACTTGGGGCTTTGCCTTCTTTGCCTGGGGA
  CCAGTTAACGTGACCTTCATGTATCTGTTTGCCATCTTTAATACCTTACAAGGATTTTTCATATTCATCT
  TTTACTGTGTGGCCAAAGAAAATGTCAGGAAGCAATGGAGGCGGTATCTTTGTTGTGGAAAGTTACGGCT
  GGCTGAAAATTCTGACTGGAGTAAAACTGCTACTAATGGTTTAAAGAAGCAGACTGTAAACCAAGGAGTG
  TCCAGCTCTTCAAATTCCTTACAGTCAAGCAGTAACTCCACTAACTCCACCACACTGCTAGTGAATAATG
  ATTGCTCAGTACACGCAAGCGGGAATGGAAATGCTTCTACAGAGAGGAATGGGGTCTCTTTTAGTGTTCA
  GAATGGAGATGTGTGCCTTCACGATTTCACTGGAAAACAGCACATGTTTAACGAGAAGGAAGATTCCTGC
  AATGGGAAAGGCCGTATGGCTCTCAGAAGGACTTCAAAGCGGGGAAGCTTACACTTTATTGAGCAAATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210484 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVFSVRQCGHVGRTEEVLLTFKIFLVIICLHVVLVTSLEEDTDNSSLSPPPAKLSVVSFAPSSNGTPEVE
  TTSLNDVTLSLLPSNETGVKPQRNICNLSSICNDSAFFRGEIMFQYDKESTVPQNQHITNGTLTGVLSLS
  ELKRSELNKTLQTLSETYFIMCATAEAQSTLNCTFTIKLNNTMNACAVIAALERVKIRPMEHCCCSVRIP
  CPSSPEELEKLQCDLQDPIVCLADHPRGPPFSSSQSIPVVPRATVLSQVPKATSFAEPPDYSPVTHNVPS
  PIGEIQPLSPQPSAPIASSPAIDMPPQSETISSPMPQTHVSGTPPPVKASFSSPTVSAPANVNTTSAPPV
  QTDIVNTSSISDLENQVLQMEKALSLGSLEPNLAGEMINQVSRLLHSPPDMLAPLAQRLLKVVDDIVLQL
  NFSNTTISLTSPSLALAVIRVNASSFNTTTFVAQDPANLQVSLETQAPENSIGTITLPSSLMNNLPAHDM
  ELASRVQFNFFETPALFQDPSLENLSLISYVISSSVANLTVRNLTRNVTVTLKHINPSQDELTVRCVFWD
  LGRNGGRGGWSDNGCSVKDRRLNETICTCSHLTSFGVLLDLSRTSVLPAQMMALTFITYIGCGLSSIFLS
  VTLVTYIAFEKIRRDYPSKILIQLCAALLLLNLVFLLDSWIALYKMQGLCISVAVFLHYFLLVSFTWMGL
  EAFHMYLALVKVFNTYIRKYILKFCIVGWGVPAVVVTIILTISPDNYGLGSYGKFPNGSPDDFCWINNNA
  VFYITVVGYFCVIFLLNVSMFIVVLVQLCRIKKKKQLGAQRKTSIQDLRSIAGLTFLLGITWGFAFFAWG
  PVNVTFMYLFAIFNTLQGFFIFIFYCVAKENVRKQWRRYLCCGKLRLAENSDWSKTATNGLKKQTVNQGV
  SSSSNSLQSSSNSTNSTTLLVNNDCSVHASGNGNASTERNGVSFSVQNGDVCLHDFTGKQHMFNEKEDSC
  NGKGRMALRRTSKRGSLHFIEQM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001079859
• ORF Size: 3009 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001079859.3
• RefSeq Size: 4853 bp
• RefSeq ORF: 3012 bp
• Locus ID: 10149
• UniProt ID: Q8IZP9
• Cytogenetics: Xp22.13
• Protein Families: Druggable Genome, GPCR, Transmembrane
• MW: 110.1 kDa
• Gene Summary: This gene encodes a member of the G protein-coupled receptor family described as an epididymis-specific transmembrane protein. The encoded protein may be proteolytically processed as it contains a motif shown to be a protein scission motif in some members of this family (PMID: 11973329). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]