MRPL39 (NM_017446) Human Tagged ORF Clone

CAT#: RC210449

MRPL39 (Myc-DDK-tagged)-Human mitochondrial ribosomal protein L39 (MRPL39), nuclear gene encoding mitochondrial protein, transcript variant 1

Product Images

Circular map for RC210449

• 

  Western blot validation of overexpression lysate (Cat# LY413745) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210449 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified MRPL39 protein (Cat# TP310449). The protein was produced from HEK293T cells transfected with MRPL39 cDNA clone (Cat# RC210449) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MRPL39
• Synonyms: C21orf92; L5mt; L39mt; MRP-L5; MRPL5; MSTP003; PRED22; PRED66; RPML5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210449 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGCGCTGGCCATGGGTTCCCGGGCGCTGCGGCTCTGGCTGGTCGCACCCGGTGGCGGGATCAAAT
  GGAGATTTATAGCAACATCGCCAGCTTCTCAGCTGTCACCGACAGAATTGACAGAAATGCGGAATGATCT
  CTTTAATAAAGAGAAAGCCAGGCAGTTATCATTAACTCCCCGAACTGAGAAGATAGAAGTTAAGCATGTT
  GGGAAAACTGACCCCGGTACTGTCTTCGTGATGAATAAAAACATTTCAACTCCCTACAGTTGTGCCATGC
  ATTTAAGCGAGTGGTATTGCAGGAAGTCCATTCTGGCTCTGGTGGATGGACAGCCTTGGGACATGTATAA
  GCCTTTAACAAAGTCCTGTGAAATTAAATTTCTTACTTTCAAAGATTGTGATCCAGGAGAAGTGAATAAG
  GCATATTGGCGTTCCTGTGCTATGATGATGGGCTGTGTGATAGAGAGGGCATTCAAAGATGAATATATGG
  TCAATTTGGTCAGAGCTCCAGAAGTTCCTGTAATTTCTGGTGCCTTCTGTTATGACGTAGTTTTGGATAG
  CAAACTTGATGAGTGGATGCCAACAAAAGAGAACTTACGTTCCTTCACAAAGGATGCTCATGCTTTAATT
  TATAAAGATCTTCCATTTGAAACTCTGGAAGTTGAAGCAAAAGTGGCATTGGAAATATTTCAACACAGCA
  AGTACAAAGTAGATTTCATAGAAGAGAAGGCATCTCAGAACCCTGAGAGAATAGTCAAGCTACACAGAAT
  AGGTGACTTCATTGATGTGAGTGAGGGCCCTCTTATTCCAAGAACAAGTATTTGTTTCCAGTATGAAGTG
  TCAGCAGTTCACAATCTTCAACCCACCCAGCCAAGTCTCATACGAAGATTCCAGGGTGTGTCTTTACCTG
  TTCACTTAAGAGCACATTTTACAATATGGGATAAGCTATTGGAAAGATCTCGGAAAATGGTAACTGAAGA
  TCAAAGTAAAGCAACAGAGGAATGTACATCTACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210449 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEALAMGSRALRLWLVAPGGGIKWRFIATSPASQLSPTELTEMRNDLFNKEKARQLSLTPRTEKIEVKHV
  GKTDPGTVFVMNKNISTPYSCAMHLSEWYCRKSILALVDGQPWDMYKPLTKSCEIKFLTFKDCDPGEVNK
  AYWRSCAMMMGCVIERAFKDEYMVNLVRAPEVPVISGAFCYDVVLDSKLDEWMPTKENLRSFTKDAHALI
  YKDLPFETLEVEAKVALEIFQHSKYKVDFIEEKASQNPERIVKLHRIGDFIDVSEGPLIPRTSICFQYEV
  SAVHNLQPTQPSLIRRFQGVSLPVHLRAHFTIWDKLLERSRKMVTEDQSKATEECTST
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017446
• ORF Size: 1014 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017446.2
• RefSeq Size: 1082 bp
• RefSeq ORF: 1017 bp
• Locus ID: 54148
• UniProt ID: Q9NYK5
• Cytogenetics: 21q21.3
• Domains: TGS
• MW: 38.7 kDa
• Gene Summary: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Two transcript variants encoding distinct isoforms have been described. A pseudogene corresponding to this gene is found on chromosome 5q. [provided by RefSeq, Jul 2008]