Steroid sulfatase (STS) (NM_000351) Human Tagged ORF Clone

CAT#: RC210431

STS (Myc-DDK-tagged)-Human steroid sulfatase (microsomal), isozyme S (STS)

Product Images

Circular map for RC210431

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  Western blot validation of overexpression lysate (Cat# LY424769) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210431 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified STS protein (Cat# TP310431). The protein was produced from HEK293T cells transfected with STS cDNA clone (Cat# RC210431) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Steroid sulfatase
• Synonyms: ARSC; ARSC1; ASC; ES; SSDD; XLI
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210431 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTTTAAGGAAGATGAAGATCCCTTTCCTCCTACTGTTCTTTCTGTGGGAAGCCGAGAGCCACGCAG
  CATCAAGGCCGAACATCATCCTGGTGATGGCTGACGACCTCGGCATTGGAGATCCTGGGTGCTATGGGAA
  CAAAACTATCAGGACTCCCAATATCGACCGGTTGGCCAGTGGGGGAGTGAAACTCACTCAGCACCTGGCA
  GCATCACCGCTGTGCACACCAAGCAGGGCAGCCTTCATGACTGGCCGGTACCCTGTCCGATCAGGAATGG
  CATCTTGGTCCCGCACTGGAGTTTTCCTCTTCACAGCCTCTTCGGGAGGACTTCCCACCGATGAGATTAC
  CTTTGCTAAGCTTCTGAAGGATCAAGGTTATTCAACAGCACTGATAGGGAAATGGCACCTTGGGATGAGC
  TGTCACAGCAAGACTGACTTCTGTCACCACCCTTTACATCACGGCTTCAATTATTTCTATGGGATCTCTT
  TGACCAATCTGAGAGACTGCAAGCCCGGAGAGGGCAGTGTCTTCACCACGGGCTTCAAGAGGCTGGTCTT
  CCTCCCCCTGCAGATCGTCGGGGTCACCCTCCTTACCCTTGCTGCACTCAATTGTCTGGGGCTACTCCAC
  GTGCCTCTAGGCGTTTTTTTCAGCCTTCTCTTCCTAGCAGCCCTAATCCTGACCCTTTTCTTGGGCTTCC
  TTCATTACTTCCGGCCCCTGAACTGCTTCATGATGAGGAACTACGAGATCATTCAGCAGCCCATGTCCTA
  TGACAATCTCACCCAGAGGCTAACGGTGGAGGCGGCCCAGTTCATACAGCGGAACACTGAGACTCCGTTC
  CTGCTTGTCTTGTCCTACCTCCACGTGCACACAGCCCTGTTCTCCAGCAAAGACTTTGCTGGCAAAAGTC
  AACACGGAGTCTACGGGGATGCTGTTGAGGAAATGGACTGGAGTGTGGGGCAGATCTTGAACCTTCTGGA
  TGAGCTGAGATTGGCTAATGATACCCTCATCTACTTCACATCGGACCAGGGAGCACATGTAGAAGAAGTG
  TCTTCCAAAGGAGAAATTCATGGCGGAAGTAATGGGATCTATAAAGGAGGAAAAGCAAACAACTGGGAAG
  GAGGTATCCGGGTTCCAGGCATCCTTCGTTGGCCCAGGGTGATACAGGCTGGCCAGAAGATTGATGAGCC
  CACTAGCAACATGGACATATTTCCTACAGTAGCCAAGCTGGCTGGAGCTCCCTTGCCTGAGGACAGGATC
  ATTGATGGACGTGATCTGATGCCCCTGCTTGAAGGAAAAAGCCAACGCTCCGATCATGAGTTTCTCTTCC
  ATTACTGCAACGCCTACTTAAATGCTGTGCGCTGGCACCCTCAGAACAGCACATCCATCTGGAAGGCCTT
  TTTCTTCACCCCCAACTTCAACCCCGTGGGTTCCAACGGATGCTTTGCCACACACGTGTGCTTCTGTTTC
  GGGAGTTATGTCACCCATCACGACCCACCTTTACTCTTTGATATTTCCAAAGATCCCAGAGAGAGAAACC
  CACTAACTCCAGCATCCGAGCCCCGGTTTTATGAAATCCTCAAAGTCATGCAGGAAGCTGCGGACAGACA
  CACCCAGACCCTGCCAGAGGTGCCCGATCAGTTTTCATGGAACAACTTTCTTTGGAAGCCCTGGCTTCAG
  CTGTGCTGTCCTTCCACCGGCCTGTCTTGCCAGTGTGATAGAGAAAAACAGGATAAGAGACTGAGCCGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210431 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPLRKMKIPFLLLFFLWEAESHAASRPNIILVMADDLGIGDPGCYGNKTIRTPNIDRLASGGVKLTQHLA
  ASPLCTPSRAAFMTGRYPVRSGMASWSRTGVFLFTASSGGLPTDEITFAKLLKDQGYSTALIGKWHLGMS
  CHSKTDFCHHPLHHGFNYFYGISLTNLRDCKPGEGSVFTTGFKRLVFLPLQIVGVTLLTLAALNCLGLLH
  VPLGVFFSLLFLAALILTLFLGFLHYFRPLNCFMMRNYEIIQQPMSYDNLTQRLTVEAAQFIQRNTETPF
  LLVLSYLHVHTALFSSKDFAGKSQHGVYGDAVEEMDWSVGQILNLLDELRLANDTLIYFTSDQGAHVEEV
  SSKGEIHGGSNGIYKGGKANNWEGGIRVPGILRWPRVIQAGQKIDEPTSNMDIFPTVAKLAGAPLPEDRI
  IDGRDLMPLLEGKSQRSDHEFLFHYCNAYLNAVRWHPQNSTSIWKAFFFTPNFNPVGSNGCFATHVCFCF
  GSYVTHHDPPLLFDISKDPRERNPLTPASEPRFYEILKVMQEAADRHTQTLPEVPDQFSWNNFLWKPWLQ
  LCCPSTGLSCQCDREKQDKRLSR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000351
• ORF Size: 1749 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000351.6
• RefSeq Size: 6377 bp
• RefSeq ORF: 1737 bp
• Locus ID: 412
• UniProt ID: P08842
• Cytogenetics: Xp22.31
• Domains: Sulfatase
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Androgen and estrogen metabolism
• MW: 65.5 kDa
• Gene Summary: This gene encodes a multi-pass membrane protein that is localized to the endoplasmic reticulum. It belongs to the sulfatase family and hydrolyzes several 3-beta-hydroxysteroid sulfates, which serve as metabolic precursors for estrogens, androgens, and cholesterol. Mutations in this gene are associated with X-linked ichthyosis (XLI). Alternatively spliced transcript variants resulting from the use of different promoters have been described for this gene (PMID:17601726). [provided by RefSeq, Mar 2016]