P2Y1 (P2RY1) (NM_002563) Human Tagged ORF Clone

CAT#: RC210406

P2RY1 (Myc-DDK-tagged)-Human purinergic receptor P2Y, G-protein coupled, 1 (P2RY1)

Product Images

Circular map for RC210406

• 

  Western blot validation of overexpression lysate (Cat# LY400908) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210406 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: P2Y1
• Synonyms: P2Y1; SARCC
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210406 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCGAGGTGCTGTGGCCGGCTGTCCCCAACGGGACGGACGCTGCCTTCCTGGCCGGTCCGGGTTCGT
  CCTGGGGGAACAGCACGGTCGCCTCCACTGCCGCCGTCTCCTCGTCGTTCAAATGCGCCTTGACCAAGAC
  GGGCTTCCAGTTTTACTACCTGCCGGCTGTCTACATCTTGGTATTCATCATCGGCTTCCTGGGCAACAGC
  GTGGCCATCTGGATGTTCGTCTTCCACATGAAGCCCTGGAGCGGCATCTCCGTGTACATGTTCAATTTGG
  CTCTGGCCGACTTCTTGTACGTGCTGACTCTGCCAGCCCTGATCTTCTACTACTTCAATAAAACAGACTG
  GATCTTCGGGGATGCCATGTGTAAACTGCAGAGGTTCATCTTTCATGTGAACCTCTATGGCAGCATCTTG
  TTTCTGACATGCATCAGTGCCCACCGGTACAGCGGTGTGGTGTACCCCCTCAAGTCCCTGGGCCGGCTCA
  AAAAGAAGAATGCGATCTGTATCAGCGTGCTGGTGTGGCTCATTGTGGTGGTGGCGATCTCCCCCATCCT
  CTTCTACTCAGGTACCGGGGTCCGCAAAAACAAAACCATCACCTGTTACGACACCACCTCAGACGAGTAC
  CTGCGAAGTTATTTCATCTACAGCATGTGCACGACCGTGGCCATGTTCTGTGTCCCCTTGGTGCTGATTC
  TGGGCTGTTACGGATTAATTGTGAGAGCTTTGATTTACAAAGATCTGGACAACTCTCCTCTGAGGAGAAA
  ATCGATTTACCTGGTAATCATTGTACTGACTGTTTTTGCTGTGTCTTACATCCCTTTCCATGTGATGAAA
  ACGATGAACTTGAGGGCCCGGCTTGATTTTCAGACCCCAGCAATGTGTGCTTTCAATGACAGGGTTTATG
  CCACGTATCAGGTGACAAGAGGTCTAGCAAGTCTCAACAGTTGTGTGGACCCCATTCTCTATTTCTTGGC
  GGGAGATACTTTCAGAAGGAGACTCTCCCGAGCCACAAGGAAAGCTTCTAGAAGAAGTGAGGCAAATTTG
  CAATCCAAGAGTGAAGACATGACCCTCAATATTTTACCTGAGTTCAAGCAGAATGGAGATACAAGCCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210406 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTEVLWPAVPNGTDAAFLAGPGSSWGNSTVASTAAVSSSFKCALTKTGFQFYYLPAVYILVFIIGFLGNS
  VAIWMFVFHMKPWSGISVYMFNLALADFLYVLTLPALIFYYFNKTDWIFGDAMCKLQRFIFHVNLYGSIL
  FLTCISAHRYSGVVYPLKSLGRLKKKNAICISVLVWLIVVVAISPILFYSGTGVRKNKTITCYDTTSDEY
  LRSYFIYSMCTTVAMFCVPLVLILGCYGLIVRALIYKDLDNSPLRRKSIYLVIIVLTVFAVSYIPFHVMK
  TMNLRARLDFQTPAMCAFNDRVYATYQVTRGLASLNSCVDPILYFLAGDTFRRRLSRATRKASRRSEANL
  QSKSEDMTLNILPEFKQNGDTSL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002563
• ORF Size: 1119 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002563.5
• RefSeq Size: 3110 bp
• RefSeq ORF: 1122 bp
• Locus ID: 5028
• UniProt ID: P47900
• Cytogenetics: 3q25.2
• Domains: 7tm_1
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• MW: 42.1 kDa
• Gene Summary: The product of this gene belongs to the family of G-protein coupled receptors. This family has several receptor subtypes with different pharmacological selectivity, which overlaps in some cases, for various adenosine and uridine nucleotides. This receptor functions as a receptor for extracellular ATP and ADP. In platelets binding to ADP leads to mobilization of intracellular calcium ions via activation of phospholipase C, a change in platelet shape, and probably to platelet aggregation. [provided by RefSeq, Jul 2008]