ART4 (NM_021071) Human Tagged ORF Clone
CAT#: RC210358
ART4 (Myc-DDK-tagged)-Human ADP-ribosyltransferase 4 (Dombrock blood group) (ART4)
ORF Plasmid: tGFP
Lentiviral Particles: DDK DDK w/ Puro mGFP mGFP w/ Puro
AAV Particle: DDK
"NM_021071" in other vectors (6)
USD 198.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | ART4 |
Synonyms | ARTC4; CD297; DO; DO/ART4; DOK1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC210358 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTCCATTGATCAACAGATGCAAGAAGATTCTTCTCCCAACTACTGTACCTCCTGCAACGATGAGAA TCTGGCTCCTTGGAGGCCTGCTGCCATTCCTGCTGCTCCTCTCTGGCCTGCAGAGACCCACAGAGGGTTC TGAGGTTGCAATTAAAATCGACTTCGACTTCGCACCAGGTTCTTTTGATGATCAGTACCAAGGCTGTAGC AAACAGGTTATGGAGAAACTAACTCAAGGGGATTATTTCACAAAAGACATAGAAGCCCAGAAGAATTATT TTAGGATGTGGCAAAAAGCCCACTTAGCCTGGCTTAACCAAGGAAAAGTTCTACCCCAGAACATGACTAC CACACACGCTGTGGCTATTTTGTTTTACACATTGAACAGCAATGTTCATTCTGACTTTACTAGAGCCATG GCCTCTGTTGCCAGGACTCCACAGCAGTATGAACGTTCATTCCACTTCAAATATTTACACTACTACCTCA CCTCAGCAATCCAGCTGCTGAGGAAAGACAGCATCATGGAGAATGGCACTCTGTGCTATGAGGTGCATTA TAGGACGAAGGATGTCCACTTTAATGCCTACACAGGGGCCACCATTCGATTTGGCCAATTCCTTTCCACA TCCCTCCTGAAAGAAGAGGCACAGGAGTTTGGGAACCAGACACTATTTACCATATTCACCTGCCTGGGTG CACCTGTACAGTACTTCTCCCTCAAGAAGGAAGTCTTGATCCCTCCCTATGAGCTGTTTAAAGTTATAAA TATGAGCTACCACCCAAGAGGAAACTGGTTGCAGTTGAGGTCAACTGGGAACCTGAGCACATATAACTGT CAGCTGCTAAAAGCTTCCAGCAAGAAATGCATCCCTGATCCTATAGCTATTGCATCTCTCTCCTTTTTGA CCAGTGTCATCATCTTTTCCAAAAGCAGAGTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210358 protein sequence
Red=Cloning site Green=Tags(s) MGPLINRCKKILLPTTVPPATMRIWLLGGLLPFLLLLSGLQRPTEGSEVAIKIDFDFAPGSFDDQYQGCS KQVMEKLTQGDYFTKDIEAQKNYFRMWQKAHLAWLNQGKVLPQNMTTTHAVAILFYTLNSNVHSDFTRAM ASVARTPQQYERSFHFKYLHYYLTSAIQLLRKDSIMENGTLCYEVHYRTKDVHFNAYTGATIRFGQFLST SLLKEEAQEFGNQTLFTIFTCLGAPVQYFSLKKEVLIPPYELFKVINMSYHPRGNWLQLRSTGNLSTYNC QLLKASSKKCIPDPIAIASLSFLTSVIIFSKSRV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_021071 |
ORF Size | 942 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_021071.1 |
RefSeq Size | 1386 bp |
RefSeq ORF | 945 bp |
Locus ID | 420 |
UniProt ID | Q93070 |
Cytogenetics | 12p12.3 |
Domains | ART |
MW | 35.9 kDa |
Gene Summary | This gene encodes a protein that contains a mono-ADP-ribosylation (ART) motif. It is a member of the ADP-ribosyltransferase gene family but enzymatic activity has not been demonstrated experimentally. Antigens of the Dombrock blood group system are located on the gene product, which is glycosylphosphatidylinosotol-anchored to the erythrocyte membrane. Allelic variants, some of which lead to adverse transfusion reactions, are known. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC210358L1 | Lenti ORF clone of Human ADP-ribosyltransferase 4 (Dombrock blood group) (ART4), Myc-DDK-tagged |
USD 600.00 |
|
RC210358L2 | Lenti ORF clone of Human ADP-ribosyltransferase 4 (Dombrock blood group) (ART4), mGFP tagged |
USD 600.00 |
|
RC210358L3 | Lenti ORF clone of Human ADP-ribosyltransferase 4 (Dombrock blood group) (ART4), Myc-DDK-tagged |
USD 600.00 |
|
RC210358L4 | Lenti ORF clone of Human ADP-ribosyltransferase 4 (Dombrock blood group) (ART4), mGFP tagged |
USD 600.00 |
|
RG210358 | ART4 (tGFP-tagged) - Human ADP-ribosyltransferase 4 (Dombrock blood group) (ART4) |
USD 500.00 |
|
SC112617 | ART4 (untagged)-Human ADP-ribosyltransferase 4 (Dombrock blood group) (ART4) |
USD 300.00 |
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