DCAF15 (NM_138353) Human Tagged ORF Clone

CAT#: RC210251

DCAF15 (Myc-DDK-tagged)-Human DDB1 and CUL4 associated factor 15 (DCAF15)

Product Images

Circular map for RC210251

• 

  Western blot validation of overexpression lysate (Cat# LY408626) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210251 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DCAF15
• Synonyms: C19orf72
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210251 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCCCAGCTCGAAATCGGAGCGGAACAGCGGGGCTGGGAGCGGCGGCGGCGGCCCCGGGGGAGCCG
  GAGGGAAGCGGGCAGCAGGGCGGCGGCGGGAGCACGTCCTCAAGCAGCTGGAGCGGGTCAAGATCAGCGG
  ACAGCTCTCCCCTCGCCTCTTCCGGAAGCTGCCTCCCCGGGTGTGCGTGTCCCTCAAGAACATTGTGGAT
  GAGGACTTCCTCTATGCAGGACATATCTTCCTGGGCTTTTCCAAATGCGGCCGCTACGTCCTCTCCTACA
  CCAGCAGCAGTGGGGATGACGACTTCTCCTTCTACATCTACCATCTGTACTGGTGGGAGTTCAACGTTCA
  CAGCAAGCTCAAGCTGGTCCGGCAGGTTCGGCTATTCCAGGACGAGGAGATCTACAGCGACCTGTACCTG
  ACCGTATGCGAGTGGCCCAGCGACGCCTCCAAGGTCATCGTCTTCGGCTTCAACACCCGCTCGGCCAACG
  GGATGCTCATGAACATGATGATGATGAGTGACGAGAACCACCGTGACATCTACGTCAGCACCGTGGCCGT
  GCCACCGCCAGGCCGCTGTGCTGCTTGCCAGGATGCCAGCCGAGCCCACCCAGGAGACCCGAATGCACAG
  TGCCTACGGCATGGCTTCATGCTGCACACCAAGTACCAGGTGGTCTACCCCTTCCCCACCTTCCAGCCCG
  CCTTCCAGCTCAAGAAGGACCAGGTGGTGCTGCTCAACACCAGCTACTCCCTGGTGGCCTGCGCCGTCTC
  CGTCCACTCGGCAGGTGACAGGAGTTTCTGCCAAATCCTGTATGACCACAGCACCTGCCCCCTGGCGCCT
  GCCAGCCCCCCTGAGCCCCAGAGCCCAGAGCTGCCCCCTGCCCTCCCCAGCTTCTGCCCTGAGGCGGCCC
  CAGCCCGTTCTTCTGGGTCTCCTGAGCCCTCGCCCGCCATTGCCAAAGCCAAGGAGTTTGTGGCTGACAT
  CTTCCGCCGGGCCAAAGAGGCCAAGGGCGGGGTCCCTGAGGAAGCCCGGCCTGCCCTGTGCCCAGGACCC
  TCTGGCAGCCGCTGCCGTGCGCACTCTGAGCCCCTAGCCCTGTGTGGAGAGACGGCACCCCGGGACAGCC
  CCCCTGCCTCGGAGGCACCTGCCTCCGAGCCTGGCTATGTCAACTACACCAAGCTGTACTATGTGCTGGA
  GTCCGGAGAGGGGACGGAGCCGGAGGATGAGTTGGAGGACGACAAGATCTCCCTGCCCTTCGTGGTGACT
  GATCTTCGTGGCCGCAACCTGCGGCCCATGCGGGAGCGGACTGCTGTCCAGGGCCAGTACCTGACAGTGG
  AGCAGCTCACACTAGACTTCGAATATGTTATCAATGAGGTCATCCGCCACGACGCTACCTGGGGCCATCA
  GTTCTGTTCTTTCAGCGACTATGACATCGTCATTCTGGAGGTCTGCCCAGAAACCAACCAGGTCCTCATC
  AACATTGGCCTGCTGCTCCTGGCCTTCCCGTCCCCCACTGAGGAGGGCCAGCTCCGACCAAAGACCTATC
  ACACCAGCCTCAAGGTGGCATGGGACCTCAACACAGGGATCTTCGAGACAGTCAGTGTAGGCGACCTGAC
  TGAGGTCAAAGGGCAGACCAGCGGCAGTGTCTGGAGCTCCTACCGCAAGAGCTGCGTGGACATGGTCATG
  AAGTGGCTGGTGCCGGAGAGCAGCGGCCGCTACGTCAACAGGATGACCAATGAGGCGCTGCACAAAGGGT
  GCTCCCTGAAGGTTCTGGCGGACAGCGAGCGATATACGTGGATCGTGCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210251 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAPSSKSERNSGAGSGGGGPGGAGGKRAAGRRREHVLKQLERVKISGQLSPRLFRKLPPRVCVSLKNIVD
  EDFLYAGHIFLGFSKCGRYVLSYTSSSGDDDFSFYIYHLYWWEFNVHSKLKLVRQVRLFQDEEIYSDLYL
  TVCEWPSDASKVIVFGFNTRSANGMLMNMMMMSDENHRDIYVSTVAVPPPGRCAACQDASRAHPGDPNAQ
  CLRHGFMLHTKYQVVYPFPTFQPAFQLKKDQVVLLNTSYSLVACAVSVHSAGDRSFCQILYDHSTCPLAP
  ASPPEPQSPELPPALPSFCPEAAPARSSGSPEPSPAIAKAKEFVADIFRRAKEAKGGVPEEARPALCPGP
  SGSRCRAHSEPLALCGETAPRDSPPASEAPASEPGYVNYTKLYYVLESGEGTEPEDELEDDKISLPFVVT
  DLRGRNLRPMRERTAVQGQYLTVEQLTLDFEYVINEVIRHDATWGHQFCSFSDYDIVILEVCPETNQVLI
  NIGLLLLAFPSPTEEGQLRPKTYHTSLKVAWDLNTGIFETVSVGDLTEVKGQTSGSVWSSYRKSCVDMVM
  KWLVPESSGRYVNRMTNEALHKGCSLKVLADSERYTWIVL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_138353
• ORF Size: 1800 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_138353.3, NP_612362.2
• RefSeq Size: 2274 bp
• RefSeq ORF: 1803 bp
• Locus ID: 90379
• UniProt ID: Q66K64
• Cytogenetics: 19p13.12
• MW: 66.5 kDa
• Gene Summary: May be involved in ubiquitination and degradation through a DBB1-CUL4 E3 protein-ubiquitin ligase.[UniProtKB/Swiss-Prot Function]