FAAH2 (NM_174912) Human Tagged ORF Clone

CAT#: RC210098

FAAH2 (Myc-DDK-tagged)-Human fatty acid amide hydrolase 2 (FAAH2)

Product Images

Circular map for RC210098

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  Western blot validation of overexpression lysate (Cat# LY406395) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210098 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FAAH2
• Synonyms: AMDD
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210098 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCACCTTCATTTACCGCCCGCATTCAGTTGTTCCTCTTGCGGGCGCTAGGCTTTCTCATAGGCTTAG
  TAGGCCGAGCAGCTTTAGTCTTAGGGGGTCCAAAGTTTGCCTCAAAGACCCCTCGGCCGGTGACTGAACC
  ATTGCTTCTGCTTTCGGGGATGCAGCTGGCCAAGCTGATCCGACAGAGAAAGGTGAAATGTATAGATGTT
  GTTCAGGCTTATATCAACAGAATCAAGGACGTGAACCCAATGATCAATGGAATTGTCAAGTACAGGTTTG
  AGGAAGCGATGAAGGAGGCTCATGCTGTAGATCAAAAGCTTGCAGAGAAGCAGGAAGATGAAGCCACCCT
  GGAAAATAAATGGCCCTTCCTTGGGGTTCCTTTGACAGTCAAGGAAGCTTTCCAGCTACAAGGAATGCCC
  AATTCTTCTGGACTCATGAACCGTCGTGATGCCATTGCCAAAACAGATGCCACTGTGGTGGCATTACTGA
  AGGGAGCTGGTGCCATTCCTCTTGGCATAACCAACTGTAGTGAGTTGTGTATGTGGTATGAATCCAGTAA
  CAAGATCTATGGCCGATCAAACAACCCATATGATTTACAGCATATTGTAGGTGGAAGTTCTGGTGGTGAG
  GGCTGCACACTGGCAGCTGCCTGCTCAGTTATTGGTGTGGGCTCTGATATTGGTGGTAGCATTCGAATGC
  CTGCTTTCTTCAATGGTATATTTGGACACAAGCCTTCTCCAGGTGTGGTTCCCAACAAAGGTCAGTTTCC
  CTTGGCTGTGGGAGCCCAGGAGTTGTTTCTGTGCACTGGTCCTATGTGCCGCTATGCTGAAGACCTGGCC
  CCCATGTTGAAGGTCATGGCAGGACCTGGGATCAAAAGGTTAAAACTAGACACAAAGGTACATTTAAAAG
  ACTTAAAATTTTACTGGATGGAACATGATGGAGGCTCATTTTTAATGTCCAAAGTGGACCAAGATCTCAT
  TATGACTCAGAAAAAGGTTGTGGTTCACCTTGAAACTATTCTAGGAGCCTCAGTTCAACATGTTAAACTG
  AAGAAAATGAAGTACTCTTTTCAGTTGTGGATCGCAATGATGTCAGCAAAGGGACATGATGGGAAGGAAC
  CTGTGAAATTTGTAGATTTGCTTGGTGACCATGGGAAACATGTCAGTCCTCTGTGGGAGTTGATCAAATG
  GTGCCTGGGTCTGTCAGTGTACACCATCCCTTCCATTGGACTGGCTTTGTTGGAAGAAAAGCTCAGATAT
  AGCAATGAGAAATACCAAAAGTTTAAGGCAGTGGAAGAAAGCCTGCGTAAAGAGCTGGTGGATATGCTAG
  GTGATGATGGTGTGTTCTTATATCCCTCACATCCCACAGTGGCACCTAAGCATCATGTCCCTCTAACACG
  GCCCTTCAACTTTGCTTACACAGGTGTCTTCAGTGCCCTGGGTTTGCCTGTGACCCAATGCCCACTGGGA
  CTGAATGCCAAAGGACTCCCTTTAGGCATCCAGGTTGTGGCTGGACCCTTTAATGATCATCTGACCCTGG
  CTGTGGCCCAGTACTTGGAGAAAACTTTTGGGGGCTGGGTCTGTCCAGGAAAGTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210098 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAPSFTARIQLFLLRALGFLIGLVGRAALVLGGPKFASKTPRPVTEPLLLLSGMQLAKLIRQRKVKCIDV
  VQAYINRIKDVNPMINGIVKYRFEEAMKEAHAVDQKLAEKQEDEATLENKWPFLGVPLTVKEAFQLQGMP
  NSSGLMNRRDAIAKTDATVVALLKGAGAIPLGITNCSELCMWYESSNKIYGRSNNPYDLQHIVGGSSGGE
  GCTLAAACSVIGVGSDIGGSIRMPAFFNGIFGHKPSPGVVPNKGQFPLAVGAQELFLCTGPMCRYAEDLA
  PMLKVMAGPGIKRLKLDTKVHLKDLKFYWMEHDGGSFLMSKVDQDLIMTQKKVVVHLETILGASVQHVKL
  KKMKYSFQLWIAMMSAKGHDGKEPVKFVDLLGDHGKHVSPLWELIKWCLGLSVYTIPSIGLALLEEKLRY
  SNEKYQKFKAVEESLRKELVDMLGDDGVFLYPSHPTVAPKHHVPLTRPFNFAYTGVFSALGLPVTQCPLG
  LNAKGLPLGIQVVAGPFNDHLTLAVAQYLEKTFGGWVCPGKF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_174912
• ORF Size: 1596 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_174912.3
• RefSeq Size: 2039 bp
• RefSeq ORF: 1599 bp
• Locus ID: 158584
• UniProt ID: Q6GMR7
• Cytogenetics: Xp11.21
• Protein Families: Transmembrane
• MW: 58.3 kDa
• Gene Summary: This gene encodes a fatty acid amide hydrolase that shares a conserved protein motif with the amidase signature family of enzymes. The encoded enzyme is able to catalyze the hydrolysis of a broad range of bioactive lipids, including those from the three main classes of fatty acid amides; N-acylethanolamines, fatty acid primary amides and N-acyl amino acids. This enzyme has a preference for monounsaturated acyl chains as a substrate. Alternate splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2017]