TYW3 (NM_138467) Human Tagged ORF Clone

CAT#: RC210021

TYW3 (Myc-DDK-tagged)-Human tRNA-yW synthesizing protein 3 homolog (S. cerevisiae) (TYW3), transcript variant 1

Product Images

Circular map for RC210021

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  Western blot validation of overexpression lysate (Cat# LY408593) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210021 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified TYW3 protein (Cat# TP310021). The protein was produced from HEK293T cells transfected with TYW3 cDNA clone (Cat# RC210021) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TYW3
• Synonyms: C1orf171
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210021 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCGCAGCGCGGAGTTCAGGAAATGGAAGGCGCAATGTTTGAGCAAAGCGGACCTCAGCCGGAAGG
  GCAGTGTTGACGAGGATGTGGTAGAGCTTGTGCAGTTTCTGAACATGCGAGATCAGTTTTTCACCACCAG
  CTCCTGCGCTGGCCGCATCCTACTCCTTGACCGGGGTATAAATGGTTTTGAGGTTCAGAAACAAAACTGT
  TGCTGGCTACTGGTTACACACAAACTTTGTGTAAAAGATGATGTGATTGTAGCTCTGAAGAAAGCAAATG
  GTGATGCCACTTTGAAATTTGAACCATTTGTTCTTCATGTGCAGTGTCGACAATTGCAGGATGCACAGAT
  TCTGCATTCCATGGCAATAGATTCTGGTTTCAGGAACTCTGGCATAACGGTGGGAAAGAGAGGAAAAACT
  ATGTTGGTTGTCCGGAGTACACATGGCTTAGAAGTTCCATTAAGCCATAAGGGAAAACTGATGGTGACAG
  AGGAATATATTGACTTCCTGTTAAATGTGGCAAATCAAAAAATGGAGGAAAACAAGAAAAGAATTGAGAG
  GTTTTACAACTGCCTACAGCATGCTTTGGAAAGGGAAACGATGACTAACTTACATCCCAAGATCAAAGAG
  AAAAATAACTCATCATATATTCATAAGAAAAAAAGAAACCCAGAAAAAACACGTGCCCAGTGTATTACTA
  AAGAAAGTGATGAAGAACTTGAAAATGATGATGATGATGATCTAGGAATCAATGTTACCATCTTCCCTGA
  AGATTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210021 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDRSAEFRKWKAQCLSKADLSRKGSVDEDVVELVQFLNMRDQFFTTSSCAGRILLLDRGINGFEVQKQNC
  CWLLVTHKLCVKDDVIVALKKANGDATLKFEPFVLHVQCRQLQDAQILHSMAIDSGFRNSGITVGKRGKT
  MLVVRSTHGLEVPLSHKGKLMVTEEYIDFLLNVANQKMEENKKRIERFYNCLQHALERETMTNLHPKIKE
  KNNSSYIHKKKRNPEKTRAQCITKESDEELENDDDDDLGINVTIFPEDY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_138467
• ORF Size: 777 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_138467.3
• RefSeq Size: 3448 bp
• RefSeq ORF: 780 bp
• Locus ID: 127253
• UniProt ID: Q6IPR3
• Cytogenetics: 1p31.1
• MW: 29.8 kDa
• Gene Summary: Wybutosine (yW) is a hypermodified guanosine at the 3-prime position adjacent to the anticodon of phenylalanine tRNA that stabilizes codon-anticodon interactions during decoding on the ribosome. TYW3 is the human homolog of a yeast gene essential for yW synthesis (Noma and Suzuki, 2006).[supplied by OMIM, Mar 2008]