XAF1 (NM_017523) Human Tagged ORF Clone
CAT#: RC209936
XAF1 (Myc-DDK-tagged)-Human XIAP associated factor 1 (XAF1), transcript variant 1
ORF Plasmid: tGFP
Lentiviral Particles: DDK DDK w/ Puro mGFP mGFP w/ Puro
AAV Particle: DDK
"NM_017523" in other vectors (7)
USD 198.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | XAF1 |
Synonyms | BIRC4BP; HSXIAPAF1; XIAPAF1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC209936 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGGAGACTTCTCGGTGTGCAGGAACTGTAAAAGACATGTAGTCTCTGCCAACTTCACCCTCCATG AGGCTTACTGCCTGCGGTTCCTGGTCCTGTGTCCGGAGTGTGAGGAGCCTGTCCCCAAGGAAACCATGGA GGAGCACTGCAAGCTTGAGCACCAGCAGGTTGGGTGTACGATGTGTCAGCAGAGCATGCAGAAGTCCTCG CTGGAGTTTCATAAGGCCAATGAGTGCCAGGAGCGCCCTGTTGAGTGTAAGTTCTGCAAACTGGACATGC AGCTCAGCAAGCTGGAGCTCCACGAGTCCTACTGTGGCAGCCGGACAGAGCTCTGCCAAGGCTGTGGCCA GTTCATCATGCACCGCATGCTCGCCCAGCACAGAGATGTCTGTCGCAGTGAACAGGCCCAGCTCGGGAAA GGGGAAAGAATTTCAGCTCCTGAAAGGGAAATCTACTGTCATTATTGCAACCAAATGATTCCAGAAAATA AGTATTTCCACCATATGGGTAAATGTTGTCCAGACTCAGAGTTTAAGAAACACTTTCCTGTTGGAAATCC AGAAATTCTTCCTTCATCTCTTCCAAGTCAAGCTGCTGAAAATCAAACTTCCACGATGGAGAAAGATGTT CGTCCAAAGACAAGAAGTATAAACAGATTTCCTCTTCATTCTGAAAGTTCATCAAAGAAAGCACCAAGAA GCAAAAACAAAACCTTGGATCCACTTTTGATGTCAGAGCCCAAGCCCAGGACCAGCTCCCCTAGAGGAGA TAAAGCAGCCTATGACATTCTGAGGAGATGTTCTCAGTGTGGCATCCTGCTTCCCCTGCCGATCCTAAAT CAACATCAGGAGAAATGCCGGTGGTTAGCTTCATCAAAAGGAAAACAAGTGAGAAATTTCAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC209936 protein sequence
Red=Cloning site Green=Tags(s) MEGDFSVCRNCKRHVVSANFTLHEAYCLRFLVLCPECEEPVPKETMEEHCKLEHQQVGCTMCQQSMQKSS LEFHKANECQERPVECKFCKLDMQLSKLELHESYCGSRTELCQGCGQFIMHRMLAQHRDVCRSEQAQLGK GERISAPEREIYCHYCNQMIPENKYFHHMGKCCPDSEFKKHFPVGNPEILPSSLPSQAAENQTSTMEKDV RPKTRSINRFPLHSESSSKKAPRSKNKTLDPLLMSEPKPRTSSPRGDKAAYDILRRCSQCGILLPLPILN QHQEKCRWLASSKGKQVRNFS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_017523 |
ORF Size | 903 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_017523.5 |
RefSeq Size | 3638 bp |
RefSeq ORF | 906 bp |
Locus ID | 54739 |
UniProt ID | Q6GPH4 |
Cytogenetics | 17p13.1 |
Domains | zf-TRAF |
MW | 34.6 kDa |
Gene Summary | This gene encodes a protein which binds to and counteracts the inhibitory effect of a member of the IAP (inhibitor of apoptosis) protein family. IAP proteins bind to and inhibit caspases which are activated during apoptosis. The proportion of IAPs and proteins which interfere with their activity, such as the encoded protein, affect the progress of the apoptosis signaling pathway. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC209936L1 | Lenti ORF clone of Human XIAP associated factor 1 (XAF1), transcript variant 1, Myc-DDK-tagged |
USD 600.00 |
|
RC209936L2 | Lenti ORF clone of Human XIAP associated factor 1 (XAF1), transcript variant 1, mGFP tagged |
USD 600.00 |
|
RC209936L3 | Lenti ORF clone of Human XIAP associated factor 1 (XAF1), transcript variant 1, Myc-DDK-tagged |
USD 600.00 |
|
RC209936L4 | Lenti ORF clone of Human XIAP associated factor 1 (XAF1), transcript variant 1, mGFP tagged |
USD 600.00 |
|
RG209936 | XAF1 (tGFP-tagged) - Human XIAP associated factor 1 (XAF1), transcript variant 1 |
USD 500.00 |
|
SC114091 | XAF1 (untagged)-Human XIAP associated factor 1 (XAF1), transcript variant 1 |
USD 300.00 |
|
SC322019 | XAF1 (untagged)-Human XIAP associated factor 1 (XAF1), transcript variant 1 |
USD 300.00 |
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