C1orf124 (SPRTN) (NM_032018) Human Tagged ORF Clone

CAT#: RC209745

SPRTN (Myc-DDK-tagged)-Human chromosome 1 open reading frame 124 (C1orf124), transcript variant 1

Product Images

Circular map for RC209745

• 

  Western blot validation of overexpression lysate (Cat# LY410392) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209745 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: C1orf124
• Synonyms: C1orf124; DVC1; PRO4323; spartan
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209745 representing NM_032018
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGATGACTTGATGTTGGCACTGCGGCTTCAGGAGGAGTGGAACTTGCAGGAGGCGGAGCGCGATC
  ATGCCCAGGAGTCCCTGTCGCTAGTGGACGCGTCGTGGGAGTTGGTGGACCCCACACCGGACTTGCAGGC
  ACTGTTTGTTCAGTTTAACGACCAATTCTTCTGGGGCCAGCTGGAGGCCGTCGAGGTGAAGTGGAGCGTG
  CGAATGACCCTGTGTGCTGGGATATGCAGCTATGAAGGGAAGGGTGGAATGTGTTCCATCCGTCTCAGCG
  AACCCCTTTTGAAGTTGAGGCCAAGAAAGGATCTTGTAGAGACCCTCCTGCATGAAATGATACATGCCTA
  TTTATTTGTCACTAATAACGACAAAGACCGAGAAGGGCATGGTCCAGAATTTTGTAAACATATGCATCGC
  ATCAACAGCCTGACTGGAGCCAATATAACGGTATACCATACTTTTCACGATGAGGTGGATGAGTATCGGC
  GACACTGGTGGCGCTGCAATGGGCCGTGCCAGCACAGGCCACCGTATTACGGCTATGTCAAACGAGCTAC
  TAACAGGGAACCCTCTGCTCATGACTATTGGTGGGCTGAGCACCAGAAAACCTGTGGAGGCACTTACATA
  AAAATCAAGGAACCAGAGAATTACTCAAAAAAAGGCAAAGGAAAGGCAAAACTAGGAAAGGAACCAGTAT
  TGGCCGCAGAGAATAAAGATAAACCCAACAGAGGTGAGGCCCAGCTAGTAATCCCTTTTAGTGGGAAAGG
  ATATGTTCTAGGAGAAACAAGCAATTTACCTTCACCTGGGAAACTGATCACTTCACATGCCATTAATAAA
  ACCCAAGATCTTTTAAATCAAAACCATTCAGCAAATGCTGTAAGACTTAATTCTAAAATCAAGGTGAAAT
  TTGAACAGAATGGTTCAAGTAAAAATTCTCATCTGGTCTCCCCTGCTGTTAGTAACAGTCACCAAAATGT
  TCTAAGCAACTACTTTCCTAGAGTATCATTTGCCAACCAAAAGGCTTTCAGAGGTGTGAATGGATCTCCA
  AGGATAAGTGTAACAGTTGGCAACATCCCTAAAAACTCAGTCTCTTCTAGTTCTCAGAGAAGGGTTTCAT
  CTTCTAAGATATCCCTAAGAAATTCTTCAAAAGTAACGGAATCAGCATCTGTGATGCCATCCCAGGATGT
  GAGTGGGTCTGAAGATACATTCCCAAATAAACGACCTAGGCTAGAAGATAAGACTGTTTTTGACAATTTT
  TTTATCAAGAAAGAGCAAATAAAAAGCAGTGGTAATGATCCAAAGTATAGTACAACCACAGCTCAGAATT
  CCAGCAGTTCATCCAGTCAGAGCAAAATGGTTAATTGCCCAGTTTGTCAGAATGAAGTTCTGGAGTCTCA
  GATTAATGAGCACTTGGACTGGTGCCTTGAAGGTGACAGCATCAAAGTCAAAAGCGAAGAAAGTCTT
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209745 representing NM_032018
  Red=Cloning site Green=Tags(s)
  
  MDDDLMLALRLQEEWNLQEAERDHAQESLSLVDASWELVDPTPDLQALFVQFNDQFFWGQLEAVEVKWSV
  RMTLCAGICSYEGKGGMCSIRLSEPLLKLRPRKDLVETLLHEMIHAYLFVTNNDKDREGHGPEFCKHMHR
  INSLTGANITVYHTFHDEVDEYRRHWWRCNGPCQHRPPYYGYVKRATNREPSAHDYWWAEHQKTCGGTYI
  KIKEPENYSKKGKGKAKLGKEPVLAAENKDKPNRGEAQLVIPFSGKGYVLGETSNLPSPGKLITSHAINK
  TQDLLNQNHSANAVRLNSKIKVKFEQNGSSKNSHLVSPAVSNSHQNVLSNYFPRVSFANQKAFRGVNGSP
  RISVTVGNIPKNSVSSSSQRRVSSSKISLRNSSKVTESASVMPSQDVSGSEDTFPNKRPRLEDKTVFDNF
  FIKKEQIKSSGNDPKYSTTTAQNSSSSSSQSKMVNCPVCQNEVLESQINEHLDWCLEGDSIKVKSEESL
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032018
• ORF Size: 1467 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032018.3
• RefSeq Size: 2816 bp
• RefSeq ORF: 1470 bp
• Locus ID: 83932
• UniProt ID: Q9H040
• Cytogenetics: 1q42.2
• Domains: SprT, ZnF_Rad18
• Protein Families: Druggable Genome
• MW: 55 kDa
• Gene Summary: The protein encoded by this gene may play a role in DNA repair during replication of damaged DNA. This protein recruits valosin containing protein (p97) to stalled DNA replication forks where it may prevent excessive translesional DNA synthesis and limit the number of DNA-damage induced mutations. It may also be involved in replication-related G2/M-checkpoint regulation. Deficiency of a similar protein in mouse causes chromosomal instability and progeroid phenotypes. Mutations in this gene have been associated with Ruijs-Aalfs syndrome (RJALS). Alternatively spliced transcript variants have been identified. [provided by RefSeq, Mar 2015]