NECAB1 (NM_022351) Human Tagged ORF Clone

CAT#: RC209704

NECAB1 (Myc-DDK-tagged)-Human N-terminal EF-hand calcium binding protein 1 (NECAB1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NECAB1 (RC209704, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NECAB1 (TA502546). Positive lysates LY411679 (100ug) and LC411679 (20ug) can be purchased separately from OriGene.

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY NECAB1 (Cat# RC209704, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NECAB1(Cat# TA502630). Positive lysates LY411679 (100ug) and LC411679 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY411679) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209704 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified NECAB1 protein (Cat# TP309704). The protein was produced from HEK293T cells transfected with NECAB1 cDNA clone (Cat# RC209704) using MegaTran 2.0 (Cat# TT210002).

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  HEK293T cells transfected with either RC209704 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-NECAB1 antibody (TA502546), and then analyzed by flow cytometry.

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Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NECAB1
• Synonyms: EFCBP1; STIP-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209704 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGATTCCCAGGAGACATCGCCGTCCTCCAACAACTCCTCGGAGGAGCTCAGCTCTGCTCTGCACC
  TGTCCAAGGGCATGTCGATCTTCCTCGACATACTGAGGAGAGCAGACAAAAATGATGATGGAAAATTATC
  CTTTGAAGAATTCAAAGCATATTTTGCAGATGGTGTTCTCAGTGGAGAAGAATTACACGAGCTTTTCCAT
  ACCATTGATACACATAATACTAATAATCTTGACACAGAAGAGCTATGTGAATATTTTTCTCAGCACTTGG
  GCGAGTATGAGAATGTACTAGCAGCACTTGAAGGCCTGAATCTTTCCATCCTGAAGGCAATGGGCAAAAC
  AAAGAAAGACTACCAAGAAGCCTCCAATTTGGAACAATTCGTAACTAGATTTTTATTGAAGGAAACCCTG
  AATCAGCTGCAGTCTCTCCAGAATTCCCTGGAATGTGCCATGGAAACTACTGAGGAGCAAACCCGTCAAG
  AAAGGCAAGGGCCAGCCAAGCCAGAAGTCCTGTCGATTCAATGGCCTGGAAAACGATCAAGCCGCCGAGT
  CCAGAGACACAACAGCTTCTCCCCAAACAGCCCTCAGTTTAATGTCAGCGGTCCAGGCTTATTAGAAGAA
  GACAACCAGTGGATGACCCAGATAAATAGACTCCAGAAATTAATTGATAGACTGGAAAAGAAGGATCTCA
  AACTCGAACCACCAGAAGAAGAAATTATTGAAGGGAATACTAAATCTCACATCATGCTTGTGCAGCGGCA
  GATGTCTGTGATAGAAGAGGACCTGGAAGAATTCCAGCTCGCTCTGAAACACTACGTGGAGAGTGCTTCC
  TCTCAAAGTGGATGCTTGCGTATTTCTATACAGAAGCTTTCAAATGAATCTCGCTACATGATCTATGAGT
  TCTGGGAGAATAGTAGTGTATGGAATAGCCACCTTCAGACAAATTATAGCAAGACATTCCAAAGAAGTAA
  TGTGGATTTCTTGGAAACTCCAGAACTCACATCTACAATGCTAGTTCCTGCTTCGTGGTGGATCCTGAAC
  AAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209704 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEDSQETSPSSNNSSEELSSALHLSKGMSIFLDILRRADKNDDGKLSFEEFKAYFADGVLSGEELHELFH
  TIDTHNTNNLDTEELCEYFSQHLGEYENVLAALEGLNLSILKAMGKTKKDYQEASNLEQFVTRFLLKETL
  NQLQSLQNSLECAMETTEEQTRQERQGPAKPEVLSIQWPGKRSSRRVQRHNSFSPNSPQFNVSGPGLLEE
  DNQWMTQINRLQKLIDRLEKKDLKLEPPEEEIIEGNTKSHIMLVQRQMSVIEEDLEEFQLALKHYVESAS
  SQSGCLRISIQKLSNESRYMIYEFWENSSVWNSHLQTNYSKTFQRSNVDFLETPELTSTMLVPASWWILN
  N
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022351
• ORF Size: 1053 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022351.5
• RefSeq Size: 5140 bp
• RefSeq ORF: 1056 bp
• Locus ID: 64168
• UniProt ID: Q8N987
• Cytogenetics: 8q21.3
• Domains: EFh, ABM
• MW: 40.5 kDa