NPBWR2 (NM_005286) Human Tagged ORF Clone

CAT#: RC209677

NPBWR2 (Myc-DDK-tagged)-Human neuropeptides B/W receptor 2 (NPBWR2)

Product Images

Circular map for RC209677

• 

  Western blot validation of overexpression lysate (Cat# LY401628) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209677 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NPBWR2
• Synonyms: GPR8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209677 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGGCCGCTGGGCACCCAGAGCCCCTTGACAGCAGGGGCTCCTTCTCCCTCCCCACGATGGGTGCCA
  ACGTCTCTCAGGACAATGGCACTGGCCACAATGCCACCTTCTCCGAGCCACTGCCGTTCCTCTATGTGCT
  CCTGCCCGCCGTGTACTCCGGGATCTGTGCTGTGGGGCTGACTGGCAACACGGCCGTCATCCTTGTAATC
  CTAAGGGCGCCCAAGATGAAGACGGTGACCAACGTGTTCATCCTGAACCTGGCCGTCGCCGACGGGCTCT
  TCACGCTGGTACTGCCTGTCAACATCGCGGAGCACCTGCTGCAGTACTGGCCCTTCGGGGAGCTGCTCTG
  CAAGCTGGTGCTGGCCGTCGACCACTACAACATCTTCTCCAGCATCTACTTCCTAGCCGTGATGAGCGTG
  GACCGATACCTGGTGGTGCTGGCCACCGTGAGGTCCCGCCACATGCCCTGGCGCACCTACCGGGGGGCGA
  AGGTCGCCAGCCTGTGTGTCTGGCTGGGCGTCACGGTCCTGGTTCTGCCCTTCTTCTCTTTCGCTGGCGT
  CTACAGCAACGAGCTGCAGGTCCCAAGCTGTGGGCTGAGCTTCCCGTGGCCCGAGCAGGTCTGGTTCAAG
  GCCAGCCGTGTCTACACGTTGGTCCTGGGCTTCGTGCTGCCCGTGTGCACCATCTGTGTGCTCTACACAG
  ACCTCCTGCGCAGGCTGCGGGCCGTGCGGCTCCGCTCTGGAGCCAAGGCTCTAGGCAAGGCCAGGCGGAA
  GGTGACCGTCCTGGTCCTCGTCGTGCTGGCCGTGTGCCTCCTCTGCTGGACGCCCTTCCACCTGGCCTCT
  GTCGTGGCCCTGACCACGGACCTGCCCCAGACCCCACTGGTCATCAGTATGTCCTACGTCATCACCAGCC
  TCAGCTACGCCAACTCGTGCCTGAACCCCTTCCTCTACGCCTTTCTAGATGACAACTTCCGGAAGAACTT
  CCGCAGCATATTGCGGTGCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209677 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MQAAGHPEPLDSRGSFSLPTMGANVSQDNGTGHNATFSEPLPFLYVLLPAVYSGICAVGLTGNTAVILVI
  LRAPKMKTVTNVFILNLAVADGLFTLVLPVNIAEHLLQYWPFGELLCKLVLAVDHYNIFSSIYFLAVMSV
  DRYLVVLATVRSRHMPWRTYRGAKVASLCVWLGVTVLVLPFFSFAGVYSNELQVPSCGLSFPWPEQVWFK
  ASRVYTLVLGFVLPVCTICVLYTDLLRRLRAVRLRSGAKALGKARRKVTVLVLVVLAVCLLCWTPFHLAS
  VVALTTDLPQTPLVISMSYVITSLSYANSCLNPFLYAFLDDNFRKNFRSILRC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005286
• ORF Size: 1001 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005286.4
• RefSeq Size: 1002 bp
• RefSeq ORF: 1002 bp
• Locus ID: 2832
• UniProt ID: P48146
• Cytogenetics: 20q13.33
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• MW: 36.9 kDa
• Gene Summary: The protein encoded by this gene is an integral membrane protein and G protein-coupled receptor. The encoded protein is similar in sequence to another G protein-coupled receptor (GPR7), and it is structurally similar to opioid and somatostatin receptors. This protein binds neuropeptides B and W. This gene is intronless and is expressed primarily in the frontal cortex of the brain. [provided by RefSeq, Jul 2008]