OTUB2 (NM_023112) Human Tagged ORF Clone

CAT#: RC209650

OTUB2 (Myc-DDK-tagged)-Human OTU domain, ubiquitin aldehyde binding 2 (OTUB2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY OTUB2 (Cat# RC209650, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OTUB2(Cat# TA501946). Positive lysates LY402958 (100ug) and LC402958 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY402958) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209650 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified OTUB2 protein (Cat# TP309650). The protein was produced from HEK293T cells transfected with OTUB2 cDNA clone (Cat# RC209650) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: OTUB2
• Synonyms: C14orf137; OTB2; OTU2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209650 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGAAACATCTTTCAACCTAATATCAGAAAAATGTGACATTCTATCCATTCTTCGGGACCATCCTG
  AAAACAGGATTTACCGGAGGAAAATCGAGGAACTCAGCAAAAGGTTCACCGCCATCCGCAAGACCAAAGG
  GGATGGGAACTGCTTCTACAGGGCCTTGGGCTATTCCTACCTGGAGTCCCTGCTGGGGAAGAGCAGGGAG
  ATCTTCAAGTTCAAAGAACGCGTACTGCAGACCCCAAATGACCTTCTGGCTGCTGGCTTTGAGGAGCACA
  AGTTCAGAAACTTCTTCAATGCTTTTTACAGTGTGGTGGAACTGGTAGAGAAGGACGGCTCAGTGTCCAG
  CCTGCTGAAGGTGTTCAACGACCAGAGTGCCTCGGACCACATCGTGCAGTTCCTGCGCCTGCTCACGTCG
  GCCTTCATCAGGAACCGAGCAGACTTCTTCCGGCACTTCATTGATGAGGAGATGGACATCAAAGACTTCT
  GCACTCACGAAGTAGAGCCCATGGCCACGGAGTGTGACCACATCCAGATCACGGCGTTGTCGCAGGCCCT
  GAGCATTGCCCTGCAAGTGGAGTACGTGGACGAGATGGATACCGCCCTGAACCACCACGTGTTCCCTGAG
  GCCGCCACCCCTTCCGTTTACCTGCTCTATAAAACATCCCACTACAACATCCTTTATGCAGCCGATAAAC
  AT
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209650 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSETSFNLISEKCDILSILRDHPENRIYRRKIEELSKRFTAIRKTKGDGNCFYRALGYSYLESLLGKSRE
  IFKFKERVLQTPNDLLAAGFEEHKFRNFFNAFYSVVELVEKDGSVSSLLKVFNDQSASDHIVQFLRLLTS
  AFIRNRADFFRHFIDEEMDIKDFCTHEVEPMATECDHIQITALSQALSIALQVEYVDEMDTALNHHVFPE
  AATPSVYLLYKTSHYNILYAADKH
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_023112
• ORF Size: 702 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_023112.1
• RefSeq Size: 3873 bp
• RefSeq ORF: 705 bp
• Locus ID: 78990
• UniProt ID: Q96DC9
• Cytogenetics: 14q32.12
• Protein Families: Protease
• MW: 27.2 kDa
• Gene Summary: This gene encodes one of several deubiquitylating enzymes. Ubiquitin modification of proteins is needed for their stability and function; to reverse the process, deubiquityling enzymes remove ubiquitin. This protein contains an OTU domain and binds Ubal (ubiquitin aldehyde); an active cysteine protease site is present in the OTU domain. [provided by RefSeq, Aug 2011]