ZCCHC3 (NM_033089) Human Tagged ORF Clone

CAT#: RC209646

ZCCHC3 (Myc-DDK-tagged)-Human zinc finger, CCHC domain containing 3 (ZCCHC3)

Product Images

Circular map for RC209646

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZCCHC3
• Synonyms: C20orf99
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209646 representing NM_033089
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCACCGGCGGCGGCGCGGAGGAAGAGAGGAAACGGGGGCGGCCGCAGCTTCTGCCCCCCGCGCGGC
  CCGCGGCCCGGGGCGAGGAGGCCGACGGCGGCCGCGAGAAGATGGGCTGGGCCCAGGTGGTGAAGAATCT
  AGCCGAGAAGAAGGGCGAATTCCGCGAGCCGCGGCCGCCGCGGCGGGAGGAGGAAAGCGGCGGCGGTGGA
  GGGAGCGCCGGGCTCGGCGGCCCCGCGGGCCTGGCGGCGCCGGACCTCGGCGACTTCCCACCGGCTGGCC
  GCGGGGATCCGAAGGGCCGTCGGAGAGATCCGGCCGGCGAGGCGGTGGACCCCCGCAAAAAGAAGGGCGC
  TGCGGAGGCGGGCAGGAGGAAGAAGGCCGAGGCGGCGGCGGCCGCCATGGCGACCCCGGCCAGGCCCGGC
  GAGGCCGAGGACGCGGCCGAGCGGCCCCTCCAGGATGAGCCGGCGGCGGCGGCAGGCCCGGGCAAGGGTC
  GCTTCCTCGTCCGCATCTGTTTCCAGGGAGACGAGGGCGCCTGCCCGACCCGGGACTTCGTGGTAGGAGC
  GCTTATCCTGCGCTCCATCGGCATGGACCCGAGCGACATCTACGCGGTCATCCAGATCCCGGGCAGCCGC
  GAATTCGACGTGAGCTTCCGCTCAGCGGAGAAGCTGGCCCTGTTCCTACGCGTCTACGAGGAGAAGCGGG
  AGCAGGAGGACTGCTGGGAGAACTTTGTGGTGCTGGGGCGGAGCAAGTCCAGCTTGAAGACGCTCTTCAT
  CCTCTTCCGGAACGAGACGGTGGACGTGGAGGACATTGTGACTTGGCTCAAGCGCCACTGCGACGTGCTG
  GCCGTGCCGGTGAAAGTGACCGACAGGTTTGGGATCTGGACCGGGGAGTACAAATGCGAGATCGAGCTGC
  GCCAGGGGGAGGGCGGGGTCAGGCACTTGCCAGGGGCCTTCTTCCTGGGGGCCGAGAGGGGCTACAGCTG
  GTACAAGGGGCAGCCCAAGACATGCTTTAAATGTGGTTCCCGGACCCACATGAGCGGCAGCTGCACGCAG
  GACAGGTGCTTCAGGTGCGGGGAGGAGGGGCACCTGAGCCCTTACTGCCGGAAGGGCATCGTGTGCAACC
  TCTGTGGCAAGCGAGGACACGCCTTTGCCCAGTGTCCCAAAGCAGTGCACAATTCCGTGGCAGCTCAGCT
  AACCGGCGTGGCCGGGCAC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209646 representing NM_033089
  Red=Cloning site Green=Tags(s)
  
  MATGGGAEEERKRGRPQLLPPARPAARGEEADGGREKMGWAQVVKNLAEKKGEFREPRPPRREEESGGGG
  GSAGLGGPAGLAAPDLGDFPPAGRGDPKGRRRDPAGEAVDPRKKKGAAEAGRRKKAEAAAAAMATPARPG
  EAEDAAERPLQDEPAAAAGPGKGRFLVRICFQGDEGACPTRDFVVGALILRSIGMDPSDIYAVIQIPGSR
  EFDVSFRSAEKLALFLRVYEEKREQEDCWENFVVLGRSKSSLKTLFILFRNETVDVEDIVTWLKRHCDVL
  AVPVKVTDRFGIWTGEYKCEIELRQGEGGVRHLPGAFFLGAERGYSWYKGQPKTCFKCGSRTHMSGSCTQ
  DRCFRCGEEGHLSPYCRKGIVCNLCGKRGHAFAQCPKAVHNSVAAQLTGVAGH
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_033089
• ORF Size: 1209 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033089.7
• RefSeq Size: 2773 bp
• RefSeq ORF: 1212 bp
• Locus ID: 85364
• UniProt ID: Q9NUD5
• Cytogenetics: 20p13
• Domains: zf-CCHC
• MW: 43.4 kDa
• Gene Summary: Nucleic acid-binding protein involved in innate immune response to DNA and RNA viruses (PubMed:30193849, PubMed:30135424). Binds DNA and RNA in the cytoplasm and acts by promoting recognition of viral nucleic acids by virus sensors, such as DDX58/RIG-I, IFIH1/MDA5 and CGAS (PubMed:30193849, PubMed:30135424). Acts as a co-sensor for recognition of double-stranded DNA (dsDNA) by cGAS in the cytoplasm, thereby playing a role in innate immune response to cytosolic dsDNA and DNA virus (PubMed:30135424). Binds dsDNA and probably acts by promoting sensing of dsDNA by CGAS, leading to enhance CGAS oligomerization and activation (PubMed:30135424). Promotes sensing of viral RNA by RIG-I-like receptors proteins DDX58/RIG-I and IFIH1/MDA5 via two mechanisms: binds double-stranded RNA (dsRNA), enhancing the binding of DDX58/RIG-I and IFIH1/MDA5 to dsRNA and promotes 'Lys-63'-linked ubiquitination and subsequent activation of DDX58/RIG-I and IFIH1/MDA5 (PubMed:30193849).[UniProtKB/Swiss-Prot Function]