COQ3 (NM_017421) Human Tagged ORF Clone

CAT#: RC209576

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COQ3 (Myc-DDK-tagged)-Human coenzyme Q3 homolog, methyltransferase (S. cerevisiae) (COQ3)


  "NM_017421" in other vectors (5)

USD 98.00

USD 460.00


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Size
    • 10 ug


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Specifications

Product Data
Product Name COQ3 (NM_017421) Human Tagged ORF Clone
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol COQ3
Synonyms bA9819.1; DHHBMT; DHHBMTASE; UG0215E05
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Cell Selection Neomycin
Sequence Data
>RC209576 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGGAGTGGCCGTAAGCTGGGCTCCTCCGGGGGTTGGTTTTTAAGAGTGCTGGGGCCTGGAGGCTGTA
ATACAAAAGCTGCGCGTCCCTTAATTTCCTCGGCGGTTTATGTGAAGAACCAGCTCAGTGGGACTCTACA
GATTAAACCAGGGGTTTTCAATGAATACAGAACCATATGGTTCAAATCCTACAGGACGATCTTTTCCTGT
TTGAACAGAATAAAGAGTTTCAGGTACCCTTGGGCGAGACTGTACAGTACTTCCCAAACCACTGTCGACA
GCGGTGAGGTAAAAACCTTCTTGGCCCTGGCTCACAAATGGTGGGATGAACAAGGAGTATATGCACCTCT
TCATTCCATGAATGACCTGAGGGTGCCATTTATTAGGGACAATCTTCTGAAAACAATTCCTAATCACCAG
CCAGGAAAACCTTTGTTGGGGATGAAGATTCTTGACGTTGGCTGTGGTGGTGGGCTGTTAACTGAACCTC
TAGGGCGGCTTGGGGCTTCAGTTATTGGAATCGACCCTGTGGATGAGAACATTAAAACAGCACAATGCCA
TAAATCATTTGATCCAGTCCTGGATAAGAGAATAGAGTACAGAGTGTGTTCCCTGGAAGAGATTGTGGAA
GAGACTGCAGAAACATTTGATGCTGTTGTAGCTTCTGAAGTTGTAGAACATGTGATTGATCTAGAAACAT
TTTTACAGTGCTGCTGTCAAGTGTTAAAACCCGGTGGTTCTTTATTCATTACTACAATCAACAAAACACA
ACTTTCCTATGCCTTGGGAATTGTTTTTTCAGAGCAAATTGCAGGTATTGTACCAAAAGGTACTCATACA
TGGGAGAAGTTTGTTTCACCTGAAACACTAGAGAGCATTCTGGAATCAAATGGTCTGTCAGTTCAAACAG
TGGTAGGAATGCTCTATAACCCCTTCTCAGGTTACTGGCATTGGAGTGAAAATACCAGCCTTAACTATGC
AGCTCATGCTGTGAAATCCAGGGTCCAGGAACACCCAGCCTCTGCTGAGTTTGTTTTAAAGGGAGAAACA
GAAGAGCTCCAAGCTAATGCCTGCACCAATCCAGCTGTGCATGAAAAGCTGAAGAAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC209576 protein sequence
Red=Cloning site Green=Tags(s)

MWSGRKLGSSGGWFLRVLGPGGCNTKAARPLISSAVYVKNQLSGTLQIKPGVFNEYRTIWFKSYRTIFSC
LNRIKSFRYPWARLYSTSQTTVDSGEVKTFLALAHKWWDEQGVYAPLHSMNDLRVPFIRDNLLKTIPNHQ
PGKPLLGMKILDVGCGGGLLTEPLGRLGASVIGIDPVDENIKTAQCHKSFDPVLDKRIEYRVCSLEEIVE
ETAETFDAVVASEVVEHVIDLETFLQCCCQVLKPGGSLFITTINKTQLSYALGIVFSEQIAGIVPKGTHT
WEKFVSPETLESILESNGLSVQTVVGMLYNPFSGYWHWSENTSLNYAAHAVKSRVQEHPASAEFVLKGET
EELQANACTNPAVHEKLKK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
Tag Myc-DDK
ACCN NM_017421
ORF Size 1107 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reference Data
RefSeq NM_017421.2, NM_017421.3, NP_059117.2, NP_059117.3
RefSeq Size 1265
RefSeq ORF 1110
Locus ID 51805
Cytogenetics 6q16.2
Protein Families Druggable Genome
Protein Pathways Metabolic pathways, Ubiquinone and other terpenoid-quinone biosynthesis
MW 41 kDa
Gene Summary Ubiquinone, also known as coenzyme Q, or Q, is a critical component of the electron transport pathways of both eukaryotes and prokaryotes (Jonassen and Clarke, 2000 [PubMed 10777520]). This lipid consists of a hydrophobic isoprenoid tail and a quinone head group. The tail varies in length depending on the organism, but its purpose is to anchor coenzyme Q to the membrane. The quinone head group is responsible for the activity of coenzyme Q in the respiratory chain. The S. cerevisiae COQ3 gene encodes an O-methyltransferase required for 2 steps in the biosynthetic pathway of coenzyme Q. This enzyme methylates an early coenzyme Q intermediate, 3,4-dihydroxy-5-polyprenylbenzoic acid, as well as the final intermediate in the pathway, converting demethyl-ubiquinone to coenzyme Q. The COQ3 gene product is also capable of methylating the distinct prokaryotic early intermediate 2-hydroxy-6-polyprenyl phenol. [supplied by OMIM, Mar 2008]

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