COQ3 (NM_017421) Human Tagged ORF Clone

CAT#: RC209576

COQ3 (Myc-DDK-tagged)-Human coenzyme Q3 homolog, methyltransferase (S. cerevisiae) (COQ3)

Product Images

Circular map for RC209576

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  Western blot validation of overexpression lysate (Cat# LY413779) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209576 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified COQ3 protein (Cat# TP309576). The protein was produced from HEK293T cells transfected with COQ3 cDNA clone (Cat# RC209576) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: COQ3
• Synonyms: bA9819.1; DHHBMT; DHHBMTASE; UG0215E05
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209576 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGAGTGGCCGTAAGCTGGGCTCCTCCGGGGGTTGGTTTTTAAGAGTGCTGGGGCCTGGAGGCTGTA
  ATACAAAAGCTGCGCGTCCCTTAATTTCCTCGGCGGTTTATGTGAAGAACCAGCTCAGTGGGACTCTACA
  GATTAAACCAGGGGTTTTCAATGAATACAGAACCATATGGTTCAAATCCTACAGGACGATCTTTTCCTGT
  TTGAACAGAATAAAGAGTTTCAGGTACCCTTGGGCGAGACTGTACAGTACTTCCCAAACCACTGTCGACA
  GCGGTGAGGTAAAAACCTTCTTGGCCCTGGCTCACAAATGGTGGGATGAACAAGGAGTATATGCACCTCT
  TCATTCCATGAATGACCTGAGGGTGCCATTTATTAGGGACAATCTTCTGAAAACAATTCCTAATCACCAG
  CCAGGAAAACCTTTGTTGGGGATGAAGATTCTTGACGTTGGCTGTGGTGGTGGGCTGTTAACTGAACCTC
  TAGGGCGGCTTGGGGCTTCAGTTATTGGAATCGACCCTGTGGATGAGAACATTAAAACAGCACAATGCCA
  TAAATCATTTGATCCAGTCCTGGATAAGAGAATAGAGTACAGAGTGTGTTCCCTGGAAGAGATTGTGGAA
  GAGACTGCAGAAACATTTGATGCTGTTGTAGCTTCTGAAGTTGTAGAACATGTGATTGATCTAGAAACAT
  TTTTACAGTGCTGCTGTCAAGTGTTAAAACCCGGTGGTTCTTTATTCATTACTACAATCAACAAAACACA
  ACTTTCCTATGCCTTGGGAATTGTTTTTTCAGAGCAAATTGCAGGTATTGTACCAAAAGGTACTCATACA
  TGGGAGAAGTTTGTTTCACCTGAAACACTAGAGAGCATTCTGGAATCAAATGGTCTGTCAGTTCAAACAG
  TGGTAGGAATGCTCTATAACCCCTTCTCAGGTTACTGGCATTGGAGTGAAAATACCAGCCTTAACTATGC
  AGCTCATGCTGTGAAATCCAGGGTCCAGGAACACCCAGCCTCTGCTGAGTTTGTTTTAAAGGGAGAAACA
  GAAGAGCTCCAAGCTAATGCCTGCACCAATCCAGCTGTGCATGAAAAGCTGAAGAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209576 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MWSGRKLGSSGGWFLRVLGPGGCNTKAARPLISSAVYVKNQLSGTLQIKPGVFNEYRTIWFKSYRTIFSC
  LNRIKSFRYPWARLYSTSQTTVDSGEVKTFLALAHKWWDEQGVYAPLHSMNDLRVPFIRDNLLKTIPNHQ
  PGKPLLGMKILDVGCGGGLLTEPLGRLGASVIGIDPVDENIKTAQCHKSFDPVLDKRIEYRVCSLEEIVE
  ETAETFDAVVASEVVEHVIDLETFLQCCCQVLKPGGSLFITTINKTQLSYALGIVFSEQIAGIVPKGTHT
  WEKFVSPETLESILESNGLSVQTVVGMLYNPFSGYWHWSENTSLNYAAHAVKSRVQEHPASAEFVLKGET
  EELQANACTNPAVHEKLKK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017421
• ORF Size: 1107 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017421.4
• RefSeq Size: 1265 bp
• RefSeq ORF: 1110 bp
• Locus ID: 51805
• UniProt ID: Q9NZJ6
• Cytogenetics: 6q16.2
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Ubiquinone and other terpenoid-quinone biosynthesis
• MW: 41 kDa
• Gene Summary: Ubiquinone, also known as coenzyme Q, or Q, is a critical component of the electron transport pathways of both eukaryotes and prokaryotes (Jonassen and Clarke, 2000 [PubMed 10777520]). This lipid consists of a hydrophobic isoprenoid tail and a quinone head group. The tail varies in length depending on the organism, but its purpose is to anchor coenzyme Q to the membrane. The quinone head group is responsible for the activity of coenzyme Q in the respiratory chain. The S. cerevisiae COQ3 gene encodes an O-methyltransferase required for 2 steps in the biosynthetic pathway of coenzyme Q. This enzyme methylates an early coenzyme Q intermediate, 3,4-dihydroxy-5-polyprenylbenzoic acid, as well as the final intermediate in the pathway, converting demethyl-ubiquinone to coenzyme Q. The COQ3 gene product is also capable of methylating the distinct prokaryotic early intermediate 2-hydroxy-6-polyprenyl phenol.[supplied by OMIM, Mar 2008]