BOLA1 (NM_016074) Human Tagged ORF Clone

CAT#: RC209532

BOLA1 (Myc-DDK-tagged)-Human bolA homolog 1 (E. coli) (BOLA1)

Product Images

Circular map for RC209532

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  Coomassie blue staining of purified BOLA1 protein (Cat# TP309532). The protein was produced from HEK293T cells transfected with BOLA1 cDNA clone (Cat# RC209532) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BOLA1
• Synonyms: CGI-143
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209532 representing NM_016074
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGAGTGGGCGGCTGGTCCTGGGTCTGGTCTCCATGGCTGGCCGCGTTTGTTTGTGCCAGGGCAGCG
  CGGGATCCGGGGCCATCGGTCCGGTGGAGGCCGCCATTCGCACGAAGTTGGAGGAGGCCCTGAGCCCCGA
  GGTGCTAGAGCTTCGCAACGAGAGCGGTGGCCACGCGGTCCCGCCTGGCAGTGAGACTCACTTCCGCGTG
  GCTGTGGTGAGCTCTCGTTTCGAGGGACTGAGCCCCCTACAACGACACCGGCTGGTCCACGCAGCGCTGG
  CCGAGGAGCTGGGAGGTCCGGTCCATGCGCTGGCCATCCAGGCACGGACCCCCGCCCAGTGGAGAGAGAA
  CTCTCAGCTGGACACTAGCCCCCCATGCCTGGGTGGGAACAAGAAAACTCTAGGAACCCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209532 representing NM_016074
  Red=Cloning site Green=Tags(s)
  
  MLSGRLVLGLVSMAGRVCLCQGSAGSGAIGPVEAAIRTKLEEALSPEVLELRNESGGHAVPPGSETHFRV
  AVVSSRFEGLSPLQRHRLVHAALAEELGGPVHALAIQARTPAQWRENSQLDTSPPCLGGNKKTLGTP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016074
• ORF Size: 411 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016074.5
• RefSeq Size: 812 bp
• RefSeq ORF: 414 bp
• Locus ID: 51027
• UniProt ID: Q9Y3E2
• Cytogenetics: 1q21.2
• Domains: BolA
• Protein Families: Transcription Factors
• MW: 14.1 kDa
• Gene Summary: Acts as a mitochondrial iron-sulfur (Fe-S) cluster assembly factor that facilitates (Fe-S) cluster insertion into a subset of mitochondrial proteins (By similarity). Probably acts together with the monothiol glutaredoxin GLRX5 (PubMed:27532772). May protect cells against oxidative stress (PubMed:22746225).[UniProtKB/Swiss-Prot Function]