KLC3 (NM_177417) Human Tagged ORF Clone

CAT#: RC209427

KLC3 (Myc-DDK-tagged)-Human kinesin light chain 3 (KLC3)

Product Images

Circular map for RC209427

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  Western blot validation of overexpression lysate (Cat# LY406158) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209427 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified KLC3 protein (Cat# TP309427). The protein was produced from HEK293T cells transfected with KLC3 cDNA clone (Cat# RC209427) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KLC3
• Synonyms: KLC2; KLC2L; KLCt; KNS2B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209427 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGTGCAGGTAGCGGCTCCTGGAAGTGCAGGGCTGGGCCCAGAGCGCCTGAGCCCTGAGGAGCTGG
  TGCGGCAGACGCGGCAAGTGGTCCAGGGGCTGGAGGCGCTGCGGGCAGAGCACCATGGCCTGGCTGGGCA
  CCTGGCGGAGGCCCTGGCGGGACAGGGCCCGGCAGCCGGCTTGGAGATGCTGGAGGAAAAGCAGCAGGTG
  GTGAGCCACTCGCTGGAGGCCATCGAGCTGGGGCTGGGCGAGGCCCAGGTGCTGCTGGCCCTGTCGGCAC
  ATGTGGGTGCACTGGAGGCAGAGAAGCAGCGGCTGCGCTCGCAGGCCCGGCGGCTGGCCCAGGAGAACGT
  GTGGCTGCGGGAGGAACTGGAGGAGACGCAGCGGCGGCTTCGGGCCAGCGAGGAGTCCGTGGCCCAGCTG
  GAGGAGGAGAAGCGCCACCTGGAGTTCCTGGGGCAGCTGCGACAGTACGACCCACCGGCGGAGAGCCAGC
  AGTCTGAGTCCCCGCCTCGCCGAGACAGCCTGGCCTCCCTGTTCCCCAGCGAGGAGGAGGAGAGGAAAGG
  TCCTGAGGCCGCAGGAGCAGCAGCTGCTCAGCAGGGTGGCTATGAGATCCCTGCCCGCCTTCGGACCCTG
  CATAACCTCGTGATCCAGTACGCGGGGCAGGGCCGCTATGAGGTGGCGGTGCCTCTGTGCCGCCAGGCCT
  TGGAGGACCTGGAGCGCAGCTCGGGCCACTGCCACCCTGACGTGGCCACCATGCTCAACATCCTGGCGCT
  GGTGTACCGGGACCAGAACAAGTACAAAGAAGCCACAGACCTTCTCCATGATGCCCTGCAGATCCGGGAG
  CAGACGCTGGGCCCTGAGCACCCCGCGGTGGCCGCCACGCTCAACAACTTGGCTGTCCTCTATGGGAAGC
  GTGGGCGTTACCGGGAGGCAGAGCCCCTGTGCCAGCGCGCTTTGGAGATCCGAGAGAAGGTCCTGGGTGC
  TGACCACCCAGATGTGGCCAAGCAGCTCAACAACCTGGCCCTGCTGTGCCAGAACCAGGGCAAGTTTGAG
  GACGTGGAGCGGCACTATGCCCGGGCCCTGAGCATCTATGAGGCACTGGGCGGGCCCCATGACCCCAACG
  TGGCCAAGACCAAGAACAACCTGGCCTCAGCCTACCTGAAACAGAACAAGTATCAACAAGCGGAAGAGCT
  GTACAAAGAAATCCTCCACAAGGAGGACCTACCCGCCCCTCTCGGTGCCCCCAACACAGGCACAGCTGGT
  GACGCAGAACAGGCCCTTCGCCGCAGCAGCTCACTCTCCAAGATCCGTGAGTCTATCAGGCGAGGAAGTG
  AGAAGCTGGTCTCCCGGCTCCGAGGCGAGGCGGCGGCAGGAGCAGCCGGAATGAAGAGAGCCATGTCACT
  CAACACACTGAACGTGGATGCTCCAAGGGCTCCTGGGACTCAGTTTCCCAGCTGGCACCTGGACAAGGCC
  CCTCGGACCCTCAGCGCCAGCACCCAGGACCTGAGCCCCCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209427 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSVQVAAPGSAGLGPERLSPEELVRQTRQVVQGLEALRAEHHGLAGHLAEALAGQGPAAGLEMLEEKQQV
  VSHSLEAIELGLGEAQVLLALSAHVGALEAEKQRLRSQARRLAQENVWLREELEETQRRLRASEESVAQL
  EEEKRHLEFLGQLRQYDPPAESQQSESPPRRDSLASLFPSEEEERKGPEAAGAAAAQQGGYEIPARLRTL
  HNLVIQYAGQGRYEVAVPLCRQALEDLERSSGHCHPDVATMLNILALVYRDQNKYKEATDLLHDALQIRE
  QTLGPEHPAVAATLNNLAVLYGKRGRYREAEPLCQRALEIREKVLGADHPDVAKQLNNLALLCQNQGKFE
  DVERHYARALSIYEALGGPHDPNVAKTKNNLASAYLKQNKYQQAEELYKEILHKEDLPAPLGAPNTGTAG
  DAEQALRRSSSLSKIRESIRRGSEKLVSRLRGEAAAGAAGMKRAMSLNTLNVDAPRAPGTQFPSWHLDKA
  PRTLSASTQDLSPH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_177417
• ORF Size: 1512 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177417.3
• RefSeq Size: 1793 bp
• RefSeq ORF: 1515 bp
• Locus ID: 147700
• UniProt ID: Q6P597
• Cytogenetics: 19q13.32
• Protein Families: Druggable Genome
• MW: 55.4 kDa
• Gene Summary: This gene encodes a member of the kinesin light chain gene family. Kinesins are molecular motors involved in the transport of cargo along microtubules, and are composed of two kinesin heavy chain (KHC) and two kinesin light chain (KLC) molecules. KLCs are thought to typically be involved in binding cargo and regulating kinesin activity. In the rat, a protein similar to this gene product is expressed in post-meiotic spermatids, where it associates with structural components of sperm tails and mitochondria. [provided by RefSeq, Jul 2008]