Embigin homolog (EMB) (NM_198449) Human Tagged ORF Clone

CAT#: RC209281

EMB (Myc-DDK-tagged)-Human embigin (EMB)

Product Images

Circular map for RC209281

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Embigin homolog
• Synonyms: GP70
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209281 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCGCCCTCCCCGGCCTGCTGGAGGCCAGGGCGCGTACGCCCCGGCTGCTCCTCCTCCAGTGCCTTC
  TCGCTGCCGCGCGCCCAAGCTCGGCGGACGGCAGTGCCCCAGATTCGCCTTTTACAAGTCCACCTCTCAG
  AGAAGAAATAATGGCAAATAACTTTTCCTTGGAGAGTCATAACATATCACTGACTGAACATTCTAGTATG
  CCAGTAGAAAAAAATATCACTTTAGAAAGGCCTTCTAATGTAAATCTCACATGCCAGTTCACAACATCTG
  GGGATTTGAATGCAGTAAATGTGACTTGGAAAAAAGATGGTGAACAACTTGAGAATAATTATCTTGTCAG
  TGCAACAGGAAGCACCTTGTATACCCAATACAGGTTCACCATCATTAATAGCAAACAAATGGGAAGTTAT
  TCTTGTTTCTTTCGAGAGGAAAAGGAACAAAGGGGAACATTTAATTTCAAAGTCCCTGAACTTCATGGGA
  AAAACAAGCCATTGATCTCTTACGTAGGGGATTCTACTGTCTTGACATGTAAATGTCAAAATTGTTTTCC
  TTTAAATTGGACCTGGTACAGTAGTAATGGGAGTGTAAAGGTTCCTGTTGGTGTTCAAATGAATAAATAT
  GTGATCAATGGAACATATGCTAACGAAACAAAGCTGAAGATAACACAACTTTTGGAGGAAGATGGGGAAT
  CTTACTGGTGCCGTGCACTATTCCAATTAGGCGAGAGTGAAGAACACATTGAGCTTGTGGTGCTGAGCTA
  TTTGGTGCCCCTCAAACCATTTCTTGTAATAGTGGCTGAGGTGATTCTTTTAGTGGCCACCATTCTGCTT
  TGTGAAAAGTACACACAAAAGAAAAAGAAGCACTCAGATGAGGGGAAAGAATTTGAGCAGATTGAACAGC
  TGAAATCAGATGATAGCAATGGTATAGAAAATAATGTCCCCAGGCATAGAAAAAATGAGTCTCTGGGCCA
  G
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209281 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MRALPGLLEARARTPRLLLLQCLLAAARPSSADGSAPDSPFTSPPLREEIMANNFSLESHNISLTEHSSM
  PVEKNITLERPSNVNLTCQFTTSGDLNAVNVTWKKDGEQLENNYLVSATGSTLYTQYRFTIINSKQMGSY
  SCFFREEKEQRGTFNFKVPELHGKNKPLISYVGDSTVLTCKCQNCFPLNWTWYSSNGSVKVPVGVQMNKY
  VINGTYANETKLKITQLLEEDGESYWCRALFQLGESEEHIELVVLSYLVPLKPFLVIVAEVILLVATILL
  CEKYTQKKKKHSDEGKEFEQIEQLKSDDSNGIENNVPRHRKNESLGQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_198449
• ORF Size: 981 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198449.3
• RefSeq Size: 4309 bp
• RefSeq ORF: 984 bp
• Locus ID: 133418
• UniProt ID: Q6PCB8
• Cytogenetics: 5q11.1
• Protein Families: Transmembrane
• MW: 36.9 kDa
• Gene Summary: This gene encodes a transmembrane glycoprotein that is a member of the immunoglobulin superfamily. The encoded protein may be involved in cell growth and development by mediating interactions between the cell and extracellular matrix. A pseudogene of this gene is found on chromosome 1. [provided by RefSeq, Jan 2009]