Frizzled 6 (FZD6) (NM_003506) Human Tagged ORF Clone

CAT#: RC209270

FZD6 (Myc-DDK-tagged)-Human frizzled family receptor 6 (FZD6), transcript variant 1

Product Images

Circular map for RC209270

• 

  Western blot validation of overexpression lysate (Cat# LY432028) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC229001 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Frizzled 6
• Synonyms: FZ-6; FZ6; HFZ6; NDNC1; NDNC10
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209270 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGATGTTTACATTTTTGTTGACGTGTATTTTTCTACCCCTCCTAAGAGGGCACAGTCTCTTCACCT
  GTGAACCAATTACTGTTCCCAGATGTATGAAAATGGCCTACAACATGACGTTTTTCCCTAATCTGATGGG
  TCATTATGACCAGAGTATTGCCGCGGTGGAAATGGAGCATTTTCTTCCTCTCGCAAATCTGGAATGTTCA
  CCAAACATTGAAACTTTCCTCTGCAAAGCATTTGTACCAACCTGCATAGAACAAATTCATGTGGTTCCAC
  CTTGTCGTAAACTTTGTGAGAAAGTATATTCTGATTGCAAAAAATTAATTGACACTTTTGGGATCCGATG
  GCCTGAGGAGCTTGAATGTGACAGATTACAATACTGTGATGAGACTGTTCCTGTAACTTTTGATCCACAC
  ACAGAATTTCTTGGTCCTCAGAAGAAAACAGAACAAGTCCAAAGAGACATTGGATTTTGGTGTCCAAGGC
  ATCTTAAGACTTCTGGGGGACAAGGATATAAGTTTCTGGGAATTGACCAGTGTGCGCCTCCATGCCCCAA
  CATGTATTTTAAAAGTGATGAGCTAGAGTTTGCAAAAAGTTTTATTGGAACAGTTTCAATATTTTGTCTT
  TGTGCAACTCTGTTCACATTCCTTACTTTTTTAATTGATGTTAGAAGATTCAGATACCCAGAGAGACCAA
  TTATATATTACTCTGTCTGTTACAGCATTGTATCTCTTATGTACTTCATTGGATTTTTGCTGGGCGATAG
  CACAGCCTGCAATAAGGCAGATGAGAAGCTAGAACTTGGTGACACTGTTGTCCTAGGCTCTCAAAATAAG
  GCTTGCACCGTTTTGTTCATGCTTTTGTATTTTTTCACAATGGCTGGCACTGTGTGGTGGGTGATTCTTA
  CCATTACTTGGTTCTTAGCTGCAGGAAGAAAATGGAGTTGTGAAGCCATCGAGCAAAAAGCAGTGTGGTT
  TCATGCTGTTGCATGGGGAACACCAGGTTTCCTGACTGTTATGCTTCTTGCTCTGAACAAAGTTGAAGGA
  GACAACATTAGTGGAGTTTGCTTTGTTGGCCTTTATGACCTGGATGCTTCTCGCTACTTTGTACTCTTGC
  CACTGTGCCTTTGTGTGTTTGTTGGGCTCTCTCTTCTTTTAGCTGGCATTATTTCCTTAAATCATGTTCG
  ACAAGTCATACAACATGATGGCCGGAACCAAGAAAAACTAAAGAAATTTATGATTCGAATTGGAGTCTTC
  AGCGGCTTGTATCTTGTGCCATTAGTGACACTTCTCGGATGTTACGTCTATGAGCAAGTGAACAGGATTA
  CCTGGGAGATAACTTGGGTCTCTGATCATTGTCGTCAGTACCATATCCCATGTCCTTATCAGGCAAAAGC
  AAAAGCTCGACCAGAATTGGCTTTATTTATGATAAAATACCTGATGACATTAATTGTTGGCATCTCTGCT
  GTCTTCTGGGTTGGAAGCAAAAAGACATGCACAGAATGGGCTGGGTTTTTTAAACGAAATCGCAAGAGAG
  ATCCAATCAGTGAAAGTCGAAGAGTACTACAGGAATCATGTGAGTTTTTCTTAAAGCACAATTCTAAAGT
  TAAACACAAAAAGAAGCACTATAAACCAAGTTCACACAAGCTGAAGGTCATTTCCAAATCCATGGGAACC
  AGCACAGGAGCTACAGCAAATCATGGCACTTCTGCAGTAGCAATTACTAGCCATGATTACCTAGGACAAG
  AAACTTTGACAGAAATCCAAACCTCACCAGAAACATCAATGAGAGAGGTGAAAGCGGACGGAGCTAGCAC
  CCCCAGGTTAAGAGAACAGGACTGTGGTGAACCTGCCTCGCCAGCAGCATCCATCTCCAGACTCTCTGGG
  GAACAGGTCGACGGGAAGGGCCAGGCAGGCAGTGTATCTGAAAGTGCGCGGAGTGAAGGAAGGATTAGTC
  CAAAGAGTGATATTACTGACACTGGCCTGGCACAGAGCAACAATTTGCAGGTCCCCAGTTCTTCAGAACC
  AAGCAGCCTCAAAGGTTCCACATCTCTGCTTGTTCACCCAGTTTCAGGAGTGAGAAAAGAGCAGGGAGGT
  GGTTGTCATTCAGATACT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209270 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEMFTFLLTCIFLPLLRGHSLFTCEPITVPRCMKMAYNMTFFPNLMGHYDQSIAAVEMEHFLPLANLECS
  PNIETFLCKAFVPTCIEQIHVVPPCRKLCEKVYSDCKKLIDTFGIRWPEELECDRLQYCDETVPVTFDPH
  TEFLGPQKKTEQVQRDIGFWCPRHLKTSGGQGYKFLGIDQCAPPCPNMYFKSDELEFAKSFIGTVSIFCL
  CATLFTFLTFLIDVRRFRYPERPIIYYSVCYSIVSLMYFIGFLLGDSTACNKADEKLELGDTVVLGSQNK
  ACTVLFMLLYFFTMAGTVWWVILTITWFLAAGRKWSCEAIEQKAVWFHAVAWGTPGFLTVMLLALNKVEG
  DNISGVCFVGLYDLDASRYFVLLPLCLCVFVGLSLLLAGIISLNHVRQVIQHDGRNQEKLKKFMIRIGVF
  SGLYLVPLVTLLGCYVYEQVNRITWEITWVSDHCRQYHIPCPYQAKAKARPELALFMIKYLMTLIVGISA
  VFWVGSKKTCTEWAGFFKRNRKRDPISESRRVLQESCEFFLKHNSKVKHKKKHYKPSSHKLKVISKSMGT
  STGATANHGTSAVAITSHDYLGQETLTEIQTSPETSMREVKADGASTPRLREQDCGEPASPAASISRLSG
  EQVDGKGQAGSVSESARSEGRISPKSDITDTGLAQSNNLQVPSSSEPSSLKGSTSLLVHPVSGVRKEQGG
  GCHSDT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003506
• ORF Size: 2118 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003506.1
• RefSeq Size: 3806 bp
• RefSeq ORF: 2121 bp
• Locus ID: 8323
• UniProt ID: O60353
• Cytogenetics: 8q22.3
• Domains: FRI, Frizzled
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Basal cell carcinoma, Colorectal cancer, Melanogenesis, Pathways in cancer, Wnt signaling pathway
• MW: 79.3 kDa
• Gene Summary: This gene represents a member of the 'frizzled' gene family, which encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The protein encoded by this family member contains a signal peptide, a cysteine-rich domain in the N-terminal extracellular region, and seven transmembrane domains, but unlike other family members, this protein does not contain a C-terminal PDZ domain-binding motif. This protein functions as a negative regulator of the canonical Wnt/beta-catenin signaling cascade, thereby inhibiting the processes that trigger oncogenic transformation, cell proliferation, and inhibition of apoptosis. Alternative splicing results in multiple transcript variants, some of which do not encode a protein with a predicted signal peptide.[provided by RefSeq, Aug 2011]