ZNF281 (NM_012482) Human Tagged ORF Clone

CAT#: RC209243

ZNF281 (Myc-DDK-tagged)-Human zinc finger protein 281 (ZNF281)

Product Images

Circular map for RC209243

• 

  Western blot validation of overexpression lysate (Cat# LY415727) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209243 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZNF281
• Synonyms: GZP1; ZBP-99; ZBP99; ZNP-99
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209243 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAATCGGCAGTGGGTTCCTGAGTGGCGGCGGAGGTACCGGCAGTAGCGGTGGTAGCGGCTCCGGCG
  GCGGTGGTAGTGGCGGCGGCGGCGGCGGCGGCAGCAGCGGCAGGAGGGCAGAGATGGAACCCACCTTTCC
  CCAGGGTATGGTTATGTTCAACCACCGTCTTCCCCCGGTCACCAGCTTCACCCGGCCGGCGGGGTCGGCC
  GCCCCTCCCCCGCAATGCGTGTTATCCTCCTCTACCTCCGCAGCCCCGGCCGCTGAGCCCCCCCCTCCGC
  CAGCCCCGGACATGACTTTCAAGAAGGAGCCGGCGGCGTCAGCCGCGGCCTTCCCCTCGCAGAGGACCTC
  CTGGGGGTTCTTGCAGTCTTTGGTTAGCATCAAACAGGAGAAACCCGCGGATCCTGAGGAGCAGCAGTCC
  CACCACCACCATCACCACCACCACTATGGGGGGCTGTTCGCTGGAGCTGAAGAGAGGTCTCCAGGCCTAG
  GAGGCGGTGAAGGGGGGAGTCACGGCGTCATCCAGGACCTCAGTATTCTCCACCAGCATGTCCAGCAGCA
  ACCAGCCCAGCACCACCGTGACGTATTACTCAGCAGCAGTAGCAGGACTGATGACCACCATGGCACTGAG
  GAGCCAAAGCAGGACACTAATGTCAAAAAGGCAAAAAGGCCAAAGCCAGAATCTCAGGGAATCAAAGCCA
  AGAGGAAGCCAAGTGCATCTTCCAAACCTTCTTTGGTTGGAGATGGAGAAGGTGCCATCCTCTCCCCAAG
  TCAGAAACCTCATATCTGTGATCACTGTAGTGCTGCTTTCCGAAGCTCCTATCACCTGCGGAGACATGTC
  CTCATTCATACAGGAGAAAGACCTTTCCAGTGCAGCCAGTGTAGTATGGGTTTCATTCAGAAATACCTAC
  TACAGAGACATGAGAAAATTCATAGTAGAGAGAAGCCATTTGGATGTGATCAGTGCAGCATGAAGTTTAT
  TCAGAAGTACCATATGGAGAGACACAAGAGGACACATAGTGGAGAAAAGCCATATAAGTGTGACACTTGC
  CAACAGTATTTTTCAAGGACTGATAGATTGTTGAAGCACAGGCGCACATGTGGTGAAGTCATAGTTAAAG
  GAGCCACTAGTGCAGAACCTGGGTCATCAAACCATACCAATATGGGTAATCTGGCTGTGTTGTCTCAGGG
  AAATACAAGTTCTTCAAGGAGAAAAACAAAGTCAAAAAGCATAGCTATTGAAAATAAGGAACAGAAGACC
  GGTAAAACAAATGAATCACAAATTTCAAATAATATAAACATGCAGAGTTACTCAGTAGAAATGCCTACCG
  TGTCTTCCAGTGGAGGCATAATTGGCACTGGAATAGATGAACTGCAGAAGAGGGTGCCAAAATTGATCTT
  TAAGAAAGGAAGCAGAAAGAATACAGATAAAAACTACCTTAACTTTGTGTCACCATTACCAGACATAGTA
  GGACAGAAATCCTTGTCTGGAAAACCAAGTGGCTCACTTGGCATAGTATCAAATAATAGTGTGGAGACCA
  TTGGTCTTCTCCAAAGTACAAGTGGCAAACAAGGTCAGATAAGTAGTAATTATGATGATGCCATGCAGTT
  TTCAAAGAAAAGAAGATATTTACCAACTGCCAGCAGCAACAGTGCCTTTTCTATAAACGTAGGACACATG
  GTCTCCCAACAGTCTGTCATTCAGTCTGCAGGTGTCAGTGTTTTGGACAATGAGGCACCATTGTCACTTA
  TTGACTCCTCAGCTCTAAATGCTGAAATTAAATCTTGTCATGACAAGTCTGGAATTCCTGATGAGGTTTT
  ACAAAGTATTTTGGATCAATACTCCAACAAATCAGAAAGCCAGAAAGAGGATCCTTTCAATATTGCAGAA
  CCACGAGTGGATTTACACACCTCAGGAGAACACTCAGAATTGGTTCAAGAAGAAAATTTGAGCCCAGGCA
  CCCAAACACCTTCAAATGATAAAGCAAGTATGTTGCAAGAATACTCCAAATACCTCCAACAGGCTTTTGA
  AAAATCCACTAATGCAAGTTTTACTCTTGGACACGGTTTCCAATTTGTCAGTTTGTCTTCACCTCTCCAC
  AACCACACTTTGTTTCCAGAAAAACAAATATACACTACGTCTCCTTTGGAGTGTGGTTTCGGCCAATCTG
  TTACCTCAGTGTTGCCATCTTCATTGCCAAAGCCTCCTTTTGGGATGTTGTTTGGATCTCAGCCAGGTCT
  TTATTTGTCTGCTTTGGATGCTACACATCAGCAGTTGACACCTTCCCAGGAGCTGGATGATCTGATAGAT
  TCTCAGAAGAACTTAGAGACTTCATCAGCCTTCCAGTCCTCATCTCAGAAATTGACTAGCCAGAAGGAAC
  AGAAAAACTTAGAGTCTTCAACAGGCTTTCAGATTCCATCTCAGGAGTTAGCTAGCCAGATAGATCCTCA
  GAAAGACATAGAGCCTAGAACAACGTATCAGATTGAGAACTTTGCACAAGCGTTTGGTTCTCAGTTTAAG
  TCGGGCAGCAGGGTGCCAATGACCTTTATCACTAACTCTAATGGAGAAGTGGACCATAGAGTAAGGACTT
  CAGTGTCAGATTTCTCAGGGTATACAAATATGATGTCTGATGTAAGTGAGCCATGTAGTACAAGAGTAAA
  GACACCCACCAGCCAGAGTTACAGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209243 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKIGSGFLSGGGGTGSSGGSGSGGGGSGGGGGGGSSGRRAEMEPTFPQGMVMFNHRLPPVTSFTRPAGSA
  APPPQCVLSSSTSAAPAAEPPPPPAPDMTFKKEPAASAAAFPSQRTSWGFLQSLVSIKQEKPADPEEQQS
  HHHHHHHHYGGLFAGAEERSPGLGGGEGGSHGVIQDLSILHQHVQQQPAQHHRDVLLSSSSRTDDHHGTE
  EPKQDTNVKKAKRPKPESQGIKAKRKPSASSKPSLVGDGEGAILSPSQKPHICDHCSAAFRSSYHLRRHV
  LIHTGERPFQCSQCSMGFIQKYLLQRHEKIHSREKPFGCDQCSMKFIQKYHMERHKRTHSGEKPYKCDTC
  QQYFSRTDRLLKHRRTCGEVIVKGATSAEPGSSNHTNMGNLAVLSQGNTSSSRRKTKSKSIAIENKEQKT
  GKTNESQISNNINMQSYSVEMPTVSSSGGIIGTGIDELQKRVPKLIFKKGSRKNTDKNYLNFVSPLPDIV
  GQKSLSGKPSGSLGIVSNNSVETIGLLQSTSGKQGQISSNYDDAMQFSKKRRYLPTASSNSAFSINVGHM
  VSQQSVIQSAGVSVLDNEAPLSLIDSSALNAEIKSCHDKSGIPDEVLQSILDQYSNKSESQKEDPFNIAE
  PRVDLHTSGEHSELVQEENLSPGTQTPSNDKASMLQEYSKYLQQAFEKSTNASFTLGHGFQFVSLSSPLH
  NHTLFPEKQIYTTSPLECGFGQSVTSVLPSSLPKPPFGMLFGSQPGLYLSALDATHQQLTPSQELDDLID
  SQKNLETSSAFQSSSQKLTSQKEQKNLESSTGFQIPSQELASQIDPQKDIEPRTTYQIENFAQAFGSQFK
  SGSRVPMTFITNSNGEVDHRVRTSVSDFSGYTNMMSDVSEPCSTRVKTPTSQSYR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012482
• ORF Size: 2685 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012482.3, NP_036614.1
• RefSeq Size: 4904 bp
• RefSeq ORF: 2688 bp
• Locus ID: 23528
• UniProt ID: Q9Y2X9
• Cytogenetics: 1q32.1
• Domains: zf-C2H2
• Protein Families: ES Cell Differentiation/IPS, Stem cell - Pluripotency, Transcription Factors
• MW: 96.9 kDa
• Gene Summary: Transcription repressor that plays a role in regulation of embryonic stem cells (ESCs) differentiation. Required for ESCs differentiation and acts by mediating autorepression of NANOG in ESCs: binds to the NANOG promoter and promotes association of NANOG protein to its own promoter and recruits the NuRD complex, which deacetylates histones. Not required for establishement and maintenance of ESCs (By similarity). Represses the transcription of a number of genes including GAST, ODC1 and VIM. Binds to the G-rich box in the enhancer region of these genes.[UniProtKB/Swiss-Prot Function]