PKN beta (PKN3) (NM_013355) Human Tagged ORF Clone

CAT#: RC209134

PKN3 (Myc-DDK-tagged)-Human protein kinase N3 (PKN3)

Product Images

Circular map for RC209134

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY PKN3 (Cat# RC209134, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PKN3 (Cat# TA810388)(1:2000). Positive lysates LY415658 (100ug) and LC415658 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY415658) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209134 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PKN beta
• Synonyms: UTDP4-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209134 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGAGGGGGCGCCGCGGCAGCCTGGGCCGAGCCAGTGGCCCCCAGAGGATGAGAAGGAGGTGATCC
  GCCGGGCCATCCAGAAAGAGCTGAAGATCAAGGAGGGGGTGGAGAACCTGCGGCGCGTGGCCACAGACCG
  CCGCCACTTGGGCCATGTGCAGCAGCTGCTGCGGTCCTCCAACCGCCGCCTGGAGCAGCTGCATGGCGAG
  CTGCGGGAGCTGCACGCCCGAATCCTGCTGCCCGGCCCTGGGCCTGGCCCAGCTGAGCCTGTGGCCTCAG
  GACCCCGGCCGTGGGCAGAGCAGCTCAGGGCTCGGCACCTAGAGGCTCTCCGGAGGCAGCTGCATGTGGA
  GCTGAAGGTGAAGCAGGGGGCTGAGAACATGACCCACACGTGCGCCAGTGGCACCCCCAAGGAGAGGAAG
  CTCCTGGCAGCTGCCCAGCAGATGCTGCGGGACAGCCAGCTGAAGGTGGCCCTGCTGCGGATGAAGATCA
  GCAGCCTGGAGGCCAGTGGGTCCCCGGAGCCAGGGCCTGAGCTGCTGGCGGAGGAGCTACAGCATCGACT
  GCACGTTGAGGCAGCTGTGGCTGAGGGCGCCAAGAACGTGGTGAAACTGCTTAGTAGCCGGAGAACACAG
  GACCGCAAGGCACTGGCTGAGGCCCAGGCCCAGCTACAGGAGTCCTCTCAGAAACTGGACCTCCTGCGCC
  TGGCCTTGGAGCAGCTGCTGGAGCAACTGCCTCCTGCCCACCCTTTGCGCAGCAGAGTGACCCGAGAGTT
  GCGGGCTGCGGTGCCTGGATACCCCCAGCCTTCAGGGACACCTGTGAAGCCCACCGCCCTAACAGGGACA
  CTGCAGGTCCGCCTCCTGGGCTGTGAACAGTTGCTGACAGCCGTGCCTGGGCGCTCCCCAGCGGCCGCAC
  TGGCCAGCAGCCCCTCCGAGGGCTGGCTTCGGACCAAGGCCAAGCACCAGCGTGGCCGAGGCGAGCTTGC
  CAGCGAGGTGCTGGCTGTGCTAAAGGTGGACAACCGTGTTGTGGGGCAGACGGGCTGGGGGCAGGTGGCC
  GAACAGTCCTGGGACCAGACCTTTGTCATCCCACTGGAGCGAGCCCGTGAGCTGGAGATTGGGGTACACT
  GGCGGGACTGGCGGCAGCTATGTGGCGTGGCCTTCCTGAGACTTGAGGACTTCCTGGACAATGCCTGTCA
  CCAACTGTCCCTCAGCCTGGTACCGCAGGGACTGCTTTTTGCCCAGGTGACCTTCTGCGATCCTGTCATT
  GAGAGGCGGCCCCGGCTGCAGAGGCAGGAACGCATCTTCTCTAAACGCAGAGGCCAGGACTTCCTGAGGG
  CTTCGCAGATGAACCTCGGCATGGCGGCCTGGGGGCGCCTCGTCATGAACCTGCTGCCCCCCTGCAGCTC
  CCCGAGCACAATCAGCCCCCCTAAAGGATGCCCTCGGACCCCAACAACACTGCGAGAGGCCTCTGACCCT
  GCCACTCCCAGTAATTTCCTGCCCAAGAAGACCCCCTTGGGTGAAGAGATGACACCCCCACCCAAGCCCC
  CACGCCTCTACCTCCCCCAGGAGCCAACATCCGAGGAGACTCCGCGCACCAAACGTCCCCATATGGAGCC
  TAGGACTCGACGTGGGCCATCTCCACCAGCCTCCCCCACCAGGAAACCCCCTCGGCTTCAGGACTTCCGC
  TGCTTAGCTGTGCTGGGCCGGGGACACTTTGGGAAGGTCCTCCTGGTCCAGTTCAAGGGGACAGGGAAAT
  ACTACGCCATCAAAGCACTGAAGAAGCAGGAGGTGCTCAGCCGGGACGAGATAGAGAGCCTGTACTGCGA
  GAAGCGGATCCTGGAGGCTGTGGGCTGCACAGGGCACCCTTTCCTGCTCTCCCTCCTTGCCTGCTTCCAG
  ACCTCCAGCCATGCCTGCTTTGTGACTGAGTTTGTGCCTGGTGGTGACCTCATGATGCAGATCCACGAGG
  ATGTCTTCCCCGAGCCCCAGGCCCGCTTCTACGTGGCTTGTGTTGTCCTGGGGCTGCAGTTCTTACACGA
  GAAGAAGATCATTTACAGGGACCTGAAGTTGGATAACCTTCTGCTGGATGCCCAGGGATTCCTGAAGATC
  GCAGACTTTGGACTCTGCAAGGAAGGGATCGGCTTCGGGGACCGGACTAGCACCTTCTGTGGCACCCCGG
  AGTTCCTGGCTCCCGAGGTGCTGACCCAGGAGGCATACACACGGGCTGTGGACTGGTGGGGGCTGGGTGT
  GCTGCTCTACGAGATGCTGGTGGGTGAGTGCCCGTTCCCAGGGGACACAGAGGAAGAGGTGTTTGACTGC
  ATCGTCAACATGGACGCCCCCTACCCCGGCTTTCTGTCGGTGCAAGGGCTTGAGTTCATTCAGAAGCTCC
  TCCAGAAGTGCCCGGAGAAGCGCCTCGGGGCAGGTGAGCAGGATGCCGAGGAGATCAAGGTCCAGCCATT
  CTTCAGGACCACCAACTGGCAAGCCCTGCTCGCCCGCACCATCCAGCCCCCCTTCGTGCCTACCCTGTGT
  GGCCCTGCGGACCTGCGCTACTTTGAGGGCGAGTTCACAGGGCTGCCGCCTGCCCTGACCCCACCTGCAC
  CCCACAGCCTCCTCACTGCCCGCCAACAGGCCGCCTTCCGGGACTTCGACTTTGTGTCAGAGCGATTCCT
  GGAACCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209134 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEEGAPRQPGPSQWPPEDEKEVIRRAIQKELKIKEGVENLRRVATDRRHLGHVQQLLRSSNRRLEQLHGE
  LRELHARILLPGPGPGPAEPVASGPRPWAEQLRARHLEALRRQLHVELKVKQGAENMTHTCASGTPKERK
  LLAAAQQMLRDSQLKVALLRMKISSLEASGSPEPGPELLAEELQHRLHVEAAVAEGAKNVVKLLSSRRTQ
  DRKALAEAQAQLQESSQKLDLLRLALEQLLEQLPPAHPLRSRVTRELRAAVPGYPQPSGTPVKPTALTGT
  LQVRLLGCEQLLTAVPGRSPAAALASSPSEGWLRTKAKHQRGRGELASEVLAVLKVDNRVVGQTGWGQVA
  EQSWDQTFVIPLERARELEIGVHWRDWRQLCGVAFLRLEDFLDNACHQLSLSLVPQGLLFAQVTFCDPVI
  ERRPRLQRQERIFSKRRGQDFLRASQMNLGMAAWGRLVMNLLPPCSSPSTISPPKGCPRTPTTLREASDP
  ATPSNFLPKKTPLGEEMTPPPKPPRLYLPQEPTSEETPRTKRPHMEPRTRRGPSPPASPTRKPPRLQDFR
  CLAVLGRGHFGKVLLVQFKGTGKYYAIKALKKQEVLSRDEIESLYCEKRILEAVGCTGHPFLLSLLACFQ
  TSSHACFVTEFVPGGDLMMQIHEDVFPEPQARFYVACVVLGLQFLHEKKIIYRDLKLDNLLLDAQGFLKI
  ADFGLCKEGIGFGDRTSTFCGTPEFLAPEVLTQEAYTRAVDWWGLGVLLYEMLVGECPFPGDTEEEVFDC
  IVNMDAPYPGFLSVQGLEFIQKLLQKCPEKRLGAGEQDAEEIKVQPFFRTTNWQALLARTIQPPFVPTLC
  GPADLRYFEGEFTGLPPALTPPAPHSLLTARQQAAFRDFDFVSERFLEP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013355
• ORF Size: 2667 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013355.5
• RefSeq Size: 3385 bp
• RefSeq ORF: 2670 bp
• Locus ID: 29941
• UniProt ID: Q6P5Z2
• Cytogenetics: 9q34.11
• Protein Families: Druggable Genome, Protein Kinase
• MW: 99.4 kDa
• Gene Summary: Contributes to invasiveness in malignant prostate cancer.[UniProtKB/Swiss-Prot Function]