SCML2 (NM_006089) Human Tagged ORF Clone

CAT#: RC209060

SCML2 (Myc-DDK-tagged)-Human sex comb on midleg-like 2 (Drosophila) (SCML2), transcript variant 1

Product Images

Circular map for RC209060

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY SCML2 (Cat# RC209060, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SCML2 (Cat# TA807779)(1:2000). Positive lysates LY416870 (100ug) and LC416870 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY416870) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209060 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified SCML2 protein (Cat# TP309060). The protein was produced from HEK293T cells transfected with SCML2 cDNA clone (Cat# RC209060) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SCML2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209060 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGACAAACAGTGAATGAAGATTCCATGGATGTCAAGAAGGAGAATCAAGAGAAAACTCCTCAGTCAA
  GTACATCTTCTGTACAAAGGGATGATTTCCACTGGGAGGAGTATTTGAAAGAGACTGGGTCTATAAGTGC
  TCCTTCAGAGTGCTTCCGTCAGTCTCAGATTCCACCTGTGAATGATTTCAAAGTTGGTATGAAATTGGAA
  GCCCGTGACCCTCGCAATGCCACTTCAGTATGTATTGCTACGGTTATTGGAATTACTGGGGCCAGGTTAC
  GGTTACGACTGGATGGTAGTGACAACAGAAATGATTTTTGGAGGCTTGTCGATTCCCCAGATATACAACC
  TGTTGGGACATGTGAAAAGGAAGGAGACTTACTTCAACCTCCACTAGGGTACCAGATGAATACATCCTCC
  TGGCCGATGTTCCTCTTAAAGACACTAAATGGGTCTGAAATGGCATCTGCCACATTATTTAAGAAGGAAC
  CACCAAAGCCCCCACTAAATAATTTTAAAGTGGGGATGAAACTGGAAGCTATTGACAAAAAGAACCCGTA
  TCTCATCTGTCCTGCGACCATTGGAGATGTTAAAGGGGATGAAGTTCATATCACATTTGATGGCTGGAGT
  GGAGCTTTTGATTACTGGTGCAAGTATGATTCTCGAGATATTTTCCCAGCTGGGTGGTGTCGCCTGACAG
  GAGATGTATTACAACCCCCAGGAACTAGTGTTCCTATTGTAAAGAATATAGCAAAAACAGAGTCTTCTCC
  TTCCGAAGCAAGCCAGCATTCAATGCAGTCTCCACAGAAAACTACTCTAATATTACCAACACAGCAGGTC
  AGGAGATCAAGTCGAATTAAACCACCTGGACCTACTGCAGTCCCCAAAAGGAGCAGTTCTGTTAAAAATA
  TCACACCAAGGAAAAAAGGTCCAAACTCAGGAAAAAAGGAAAAACCTTTGCCCGTGATATGTTCTACATC
  TGCAGCTTCTCTAAAATCGCTGACCAGAGACCGTGGCATGTTATATAAAGATGTCGCTTCTGGGCCATGT
  AAAATAGTGATGTCTACAGTCTGTGTCTATGTAAACAAACATGGAAACTTTGGCCCTCATCTGGATCCCA
  AGAGAATCCAGCAGCTGCCTGACCACTTCGGCCCGGGCCCGGTGAATGTGGTGCTTCGCCGGATTGTGCA
  GGCCTGTGTGGATTGTGCCCTTGAAACTAAAACTGTTTTTGGATACCTGAAGCCAGATAATCGTGGAGGA
  GAAGTGATAACTGCCTCCTTTGATGGGGAAACTCATTCCATCCAGCTCCCTCCAGTGAACAGTGCATCAT
  TTGCTCTTCGCTTTCTTGAGAACTTCTGCCACAGTCTGCAGTGTGATAACCTTTTGAGTAGCCAGCCTTT
  TAGTTCTTCCAGGGGTCATACTCACAGCTCTGCAGAGCATGATAAAAATCAGTCAGCAAAAGAAGATGTA
  ACAGAAAGGCAAAGCACCAAACGATCTCCTCAGCAAACTGTACCATATGTTGTTCCTCTCTCTCCTAAGC
  TCCCCAAAACAAAGGAGTATGCGTCTGAAGGAGAACCATTGTTTGCTGGGGGAAGTGCCATTCCCAAAGA
  GGAGAATCTTTCAGAAGATTCTAAGAGCTCATCACTAAATTCAGGAAATTATTTGAATCCTGCCTGTAGA
  AATCCTATGTATATTCATACTTCAGTCTCCCAGGATTTTTCTCGAAGTGTGCCAGGCACCACAAGTTCAC
  CACTAGTTGGGGACATATCCCCCAAGAGCAGTCCCCATGAAGTTAAATTCCAAATGCAGAGGAAAAGTGA
  AGCTCCAAGTTATATAGCTGTACCTGATCCCAGTGTCCTGAAACAAGGCTTCTCTAAGGACCCTTCAACC
  TGGTCTGTGGATGAAGTGATACAGTTTATGAAACATACAGATCCTCAGATATCAGGCCCCCTCGCCGACC
  TCTTCAGGCAACATGAAATTGATGGGAAGGCTCTGTTCCTACTCAAGAGTGATGTGATGATGAAGTATAT
  GGGGCTGAAGCTGGGGCCAGCATTAAAGCTGTGTTACTACATTGAAAAGCTTAAAGAAGGAAAATACAGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209060 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGQTVNEDSMDVKKENQEKTPQSSTSSVQRDDFHWEEYLKETGSISAPSECFRQSQIPPVNDFKVGMKLE
  ARDPRNATSVCIATVIGITGARLRLRLDGSDNRNDFWRLVDSPDIQPVGTCEKEGDLLQPPLGYQMNTSS
  WPMFLLKTLNGSEMASATLFKKEPPKPPLNNFKVGMKLEAIDKKNPYLICPATIGDVKGDEVHITFDGWS
  GAFDYWCKYDSRDIFPAGWCRLTGDVLQPPGTSVPIVKNIAKTESSPSEASQHSMQSPQKTTLILPTQQV
  RRSSRIKPPGPTAVPKRSSSVKNITPRKKGPNSGKKEKPLPVICSTSAASLKSLTRDRGMLYKDVASGPC
  KIVMSTVCVYVNKHGNFGPHLDPKRIQQLPDHFGPGPVNVVLRRIVQACVDCALETKTVFGYLKPDNRGG
  EVITASFDGETHSIQLPPVNSASFALRFLENFCHSLQCDNLLSSQPFSSSRGHTHSSAEHDKNQSAKEDV
  TERQSTKRSPQQTVPYVVPLSPKLPKTKEYASEGEPLFAGGSAIPKEENLSEDSKSSSLNSGNYLNPACR
  NPMYIHTSVSQDFSRSVPGTTSSPLVGDISPKSSPHEVKFQMQRKSEAPSYIAVPDPSVLKQGFSKDPST
  WSVDEVIQFMKHTDPQISGPLADLFRQHEIDGKALFLLKSDVMMKYMGLKLGPALKLCYYIEKLKEGKYS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006089
• ORF Size: 2100 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006089.3
• RefSeq Size: 4212 bp
• RefSeq ORF: 2103 bp
• Locus ID: 10389
• UniProt ID: Q9UQR0
• Cytogenetics: Xp22.13
• Domains: SAM, MBT
• Protein Families: Transcription Factors
• MW: 77.3 kDa
• Gene Summary: This gene encodes a member of the Polycomb group proteins. These proteins form the Polycomb repressive complexes which are involved in transcriptional repression. The encoded protein binds histone peptides that are monomethylated at lysine residues and may be involved in regulating homeotic gene expression during development. [provided by RefSeq, Jun 2010]