U1SNRNPBP (SNRNP35) (NM_007020) Human Tagged ORF Clone

CAT#: RC208918

U1SNRNPBP (Myc-DDK-tagged)-Human U11/U12 snRNP 35K (U1SNRNPBP), transcript variant 1

Product Images

Circular map for RC208918

• 

  Western blot validation of overexpression lysate (Cat# LY416257) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208918 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U1SNRNPBP
• Synonyms: HM-1, MGC138160
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208918 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACGATTGGATGCCCATCGCCAAGGAGTATGATCCACTCAAAGCGGGCAGCATTGATGGCACCGATG
  AAGACCCACACGACCGCGCGGTCTGGAGGGCAATGCTGGCACGATATGTCCCCAACAAAGGTGTCATAGG
  AGATCCCCTCCTCACCCTGTTTGTGGCCAGACTAAACTTGCAGACCAAGGAGGACAAATTAAAGGAAGTC
  TTTTCCCGCTATGGTGACATCCGGCGGCTTCGGCTGGTCAGGGACTTGGTCACAGGTTTTTCAAAGGGCT
  ACGCCTTCATCGAATACAAGGAGGAGCGTGCCGTGATCAAAGCTTACCGAGATGCTGATGGCCTGGTTAT
  TGACCAGCATGAGATATTTGTGGACTACGAGCTGGAAAGGACTCTCAAAGGGTGGATCCCTCGGCGACTT
  GGAGGCGGTCTTGGGGGAAAAAAGGAGTCTGGGCAACTGAGATTTGGGGGACGGGACCGGCCTTTTCGAA
  AACCTATTAACTTGCCAGTTGTTAAAAACGACCTCTATAGAGAGGGAAAACGGGAAAGGCGGGAGCGATC
  TCGATCCCGAGAAAGACACTGGGACTCGAGGACAAGGGATCGAGACCATGACAGGGGCCGGGAGAAGAGA
  TGGCAAGAAAGAGAGCCGACCAGGGTGTGGCCCGACAATGACTGGGAGAGAGAGAGGGACTTCAGAGATG
  ACAGGATCAAGGGGAGGGAGAAGAAGGAAAGAGGCAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208918 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MNDWMPIAKEYDPLKAGSIDGTDEDPHDRAVWRAMLARYVPNKGVIGDPLLTLFVARLNLQTKEDKLKEV
  FSRYGDIRRLRLVRDLVTGFSKGYAFIEYKEERAVIKAYRDADGLVIDQHEIFVDYELERTLKGWIPRRL
  GGGLGGKKESGQLRFGGRDRPFRKPINLPVVKNDLYREGKRERRERSRSRERHWDSRTRDRDHDRGREKR
  WQEREPTRVWPDNDWERERDFRDDRIKGREKKERGK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007020
• ORF Size: 738 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007020.2, NP_008951.1
• RefSeq Size: 1509 bp
• RefSeq ORF: 740 bp
• Locus ID: 11066
• Cytogenetics: 12q24.31
• Domains: RRM
• MW: 29.5 kDa
• Gene Summary: The protein encoded by this gene is a homolog of the U1-snRNP binding protein. The N-terminal half contains a RNA recognition motif and the C-terminal half is rich in Arg/Asp and Arg/Glu dipeptides, which is a characteristic of a variety of splicing factors. This protein is a component of the U11/U12 small nuclear ribonucleoproteins (snRNP) that form part of the U12-type spliceosome. Alternative splicing results in multiple transcript variants encoding two distinct isoforms and representing a non-protein coding variant. [provided by RefSeq, Aug 2013]