p40 (RABEPK) (NM_005833) Human Tagged ORF Clone

CAT#: RC208763

RABEPK (Myc-DDK-tagged)-Human Rab9 effector protein with kelch motifs (RABEPK), transcript variant 1

Product Images

Circular map for RC208763

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  Western blot validation of overexpression lysate (Cat# LY417020) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208763 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified RABEPK protein (Cat# TP308763). The protein was produced from HEK293T cells transfected with RABEPK cDNA clone (Cat# RC208763) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: p40
• Synonyms: bA65N13.1; p40; RAB9P40
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208763 representing NM_005833
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCAACTGCCAGTCTTGGAACCTGGAGACAAGCCCAGGAAAGCAACATGGTACACCTTGACTGTCC
  CTGGAGACAGCCCCTGTGCTCGAGTTGGCCACAGCTGTTCATATTTACCCCCAGTTGGTAATGCCAAGAG
  AGGGAAGGTCTTCATTGTTGGGGGAGCAAATCCAAACAGAAGCTTCTCAGACGTGCACACCATGGATCTG
  GGAAAACACCAGTGGGACTTAGATACCTGCAAGGGCCTCTTGCCCCGGTATGAACATGCTAGCTTCATTC
  CCTCCTGCACACCTGACCGTATCTGGGTATTTGGAGGTGCCAACCAATCAGGAAATCGAAATTGTCTACA
  AGTCCTGAATCCTGAAACCAGGACGTGGACCACGCCAGAAGTGACCAGCCCCCCACCATCCCCAAGAACA
  TTCCACACATCATCGGCAGCCATTGGAAACCAGCTATATGTCTTTGGGGGCGGAGAGAGAGGTGCCCAGC
  CCGTGCAGGACACGAAGCTGCATGTGTTTGACGCAAACACTCTGACCTGGTCACAGCCAGAGACACTTGG
  AAATCCTCCATCTCCCCGGCATGGTCATGTGATGGTGGCAGCAGGGACAAAGCTCTTCATCCACGGAGGC
  TTGGCGGGGGACAGATTCTATGATGACCTCCACTGCATTGATATAAGTGACATGAAATGGCAGAAGCTAA
  ATCCCACTGGGGCTGCTCCAGCAGGCTGTGCTGCCCACTCAGCTGTGGCCATGGGAAAACATGTGTACAT
  CTTTGGTGGAATGACTCCTGCAGGAGCACTGGACACAATGTACCAGTATCACACAGAAGAGCAGCATTGG
  ACCTTGCTTAAATTTGATACTCTTCTACCCCCTGGACGATTGGACCATTCCATGTGTATCATTCCATGGC
  CAGTGACGTGTGCTTCTGAGAAAGAAGATTCCAACTCTCTCACTCTGAACCATGAAGCTGAGAAAGAGGA
  TTCAGCTGACAAAGTAATGAGCCACAGTGGTGACTCACATGAGGAAAGCCAGACTGCTACACTGCTCTGT
  TTGGTGTTTGGTGGGATGAATACAGAAGGGGAAATCTATGACGATTGTATTGTGACTGTAGTGGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208763 representing NM_005833
  Red=Cloning site Green=Tags(s)
  
  MKQLPVLEPGDKPRKATWYTLTVPGDSPCARVGHSCSYLPPVGNAKRGKVFIVGGANPNRSFSDVHTMDL
  GKHQWDLDTCKGLLPRYEHASFIPSCTPDRIWVFGGANQSGNRNCLQVLNPETRTWTTPEVTSPPPSPRT
  FHTSSAAIGNQLYVFGGGERGAQPVQDTKLHVFDANTLTWSQPETLGNPPSPRHGHVMVAAGTKLFIHGG
  LAGDRFYDDLHCIDISDMKWQKLNPTGAAPAGCAAHSAVAMGKHVYIFGGMTPAGALDTMYQYHTEEQHW
  TLLKFDTLLPPGRLDHSMCIIPWPVTCASEKEDSNSLTLNHEAEKEDSADKVMSHSGDSHEESQTATLLC
  LVFGGMNTEGEIYDDCIVTVVD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005833
• ORF Size: 1116 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005833.4
• RefSeq Size: 1721 bp
• RefSeq ORF: 1119 bp
• Locus ID: 10244
• UniProt ID: Q7Z6M1
• Cytogenetics: 9q33.3
• Domains: Kelch
• Protein Families: Druggable Genome
• MW: 40.6 kDa
• Gene Summary: Rab9 effector required for endosome to trans-Golgi network (TGN) transport.[UniProtKB/Swiss-Prot Function]