ZNF394 (NM_032164) Human Tagged ORF Clone

CAT#: RC208681

ZNF394 (Myc-DDK-tagged)-Human zinc finger protein 394 (ZNF394)

Product Images

Circular map for RC208681

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY ZNF394 (Cat# RC208681, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZNF394 (Cat# TA808930)(1:2000). Positive lysates LY403147 (100ug) and LC403147 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY403147) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208681 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ZNF394 protein (Cat# TP308681). The protein was produced from HEK293T cells transfected with ZNF394 cDNA clone (Cat# RC208681) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZNF394
• Synonyms: ZKSCAN14; ZSCAN46
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208681 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATTCCAGCTTGACCGCCCAGAGGCGCGGCAGTGACGCCGAGTTGGGACCCTGGGTGATGGCTGCGA
  GGTCCAAGGACGCGGCGCCGTCCCAACGCGACGGACTTTTGCCCGTGAAAGTGGAGGAAGACTCACCCGG
  AAGTTGGGAGCCCAACTATCCCGCGGCTTCGCCGGACCCCGAAACTTCTCGACTGCACTTTAGGCAGCTG
  CGTTACCAGGAGGTGGCTGGACCGGAAGAGGCGCTGAGCCGGCTCCGAGAACTCTGTCGTCGGTGGCTGA
  GACCCGAGCTGCTCTCCAAGGAGCAGATCCTGGAGCTGCTGGTGCTGGAGCAGTTCCTCACCATCCTGCC
  CGAGGAGCTTCAAGCCTGGGTGCGAGAGCACTGCCCAGAGAGCGGGGAGGAGGCGGTGGCCGTGGTGCGG
  GCTCTGCAGCGAGCGCTCGATGGAACCTCATCCCAGGGGATGGTGACTTTCGAGGACACGGCTGTGTCTC
  TAACCTGGGAGGAGTGGGAGCGCCTGGACCCAGCACGGAGGGACTTCTGCAGAGAGAGTGCGCAGAAGGA
  TTCCGGGAGCACAGTTCCGCCGAGTTTGGAAAGCAGAGTGGAGAACAAAGAGTTGATTCCAATGCAACAA
  ATTTTAGAAGAAGCGGAGCCACAGGGGCAACTACAAGAAGCGTTCCAGGGGAAGCGCCCCCTGTTTTCTA
  AGTGTGGCAGTACCCATGAGGACAGGGTGGAAAAGCAGTCCGGAGACCCCTTGCCCCTGAAACTTGAAAA
  TTCTCCTGAAGCAGAAGGACTCAACAGCATCTCAGATGTCAATAAGAATGGTTCCATAGAAGGGGAAGAC
  TCTAAAAATAATGAATTGCAGAACAGTGCCAGGTGTTCCAACCTTGTTCTATGTCAGCACATCCCGAAAG
  CAGAGAGGCCCACTGACAGTGAGGAACACGGGAACAAGTGCAAGCAAAGTTTCCACATGGTGACGTGGCA
  CGTGCTGAAACCTCACAAGTCTGACAGTGGAGACAGTTTCCATCATTCCAGCCTTTTTGAGACCCAGAGG
  CAGCTCCATGAAGAAAGACCTTATAAATGTGGTAACTGTGGGAAGAGTTTCAAACAACGCTCTGACCTCT
  TTAGACACCAGAGAATCCACACAGGTGAGAAACCCTATGGCTGCCAAGAATGTGGGAAAAGCTTCAGCCA
  GAGTGCTGCCCTGACCAAGCACCAGAGGACACACACAGGCGAGAAGCCGTACACCTGTCTGAAATGTGGG
  GAGCGCTTCAGGCAGAATTCACACCTAAATCGTCATCAAAGTACCCACAGTAGAGACAAACATTTTAAAT
  GTGAGGAATGCGGGGAAACCTGTCATATTTCCAACCTTTTTAGACATCAGAGACTACATAAAGGGGAAAG
  ACCCTATAAGTGTGAAGAATGCGAGAAGAGCTTCAAACAGCGCTCTGACCTCTTTAAACACCACAGAATC
  CACACTGGGGAGAAGCCCTATGGATGTTCCGTCTGTGGGAAACGCTTCAATCAGAGTGCAACCCTCATTA
  AACACCAGAGAATTCACACTGGGGAAAAGCCTTACAAATGTCTTGAATGTGGGGAAAGATTTAGACAAAG
  TACACACCTTATCCGACACCAAAGAATTCATCAAAATAAAGTGCTGTCGGCTGGGCGTGGTGGCTCACGC
  CTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208681 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MNSSLTAQRRGSDAELGPWVMAARSKDAAPSQRDGLLPVKVEEDSPGSWEPNYPAASPDPETSRLHFRQL
  RYQEVAGPEEALSRLRELCRRWLRPELLSKEQILELLVLEQFLTILPEELQAWVREHCPESGEEAVAVVR
  ALQRALDGTSSQGMVTFEDTAVSLTWEEWERLDPARRDFCRESAQKDSGSTVPPSLESRVENKELIPMQQ
  ILEEAEPQGQLQEAFQGKRPLFSKCGSTHEDRVEKQSGDPLPLKLENSPEAEGLNSISDVNKNGSIEGED
  SKNNELQNSARCSNLVLCQHIPKAERPTDSEEHGNKCKQSFHMVTWHVLKPHKSDSGDSFHHSSLFETQR
  QLHEERPYKCGNCGKSFKQRSDLFRHQRIHTGEKPYGCQECGKSFSQSAALTKHQRTHTGEKPYTCLKCG
  ERFRQNSHLNRHQSTHSRDKHFKCEECGETCHISNLFRHQRLHKGERPYKCEECEKSFKQRSDLFKHHRI
  HTGEKPYGCSVCGKRFNQSATLIKHQRIHTGEKPYKCLECGERFRQSTHLIRHQRIHQNKVLSAGRGGSR
  L
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032164
• ORF Size: 1683 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032164.4
• RefSeq Size: 2259 bp
• RefSeq ORF: 1686 bp
• Locus ID: 84124
• UniProt ID: Q53GI3
• Cytogenetics: 7q22.1
• Domains: KRAB, LER, zf-C2H2
• Protein Families: Transcription Factors
• MW: 64.3 kDa
• Gene Summary: The protein encoded by this gene is a zinc finger protein that inhibits the transcription of mitogen-activated protein kinase signaling pathways. The encoded protein may be involved in cardiac function. [provided by RefSeq, Sep 2016]