BYSL (NM_004053) Human Tagged ORF Clone

CAT#: RC208675

BYSL (Myc-DDK-tagged)-Human bystin-like (BYSL)

Product Images

Circular map for RC208675

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  Western blot validation of overexpression lysate (Cat# LY401313) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208675 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified BYSL protein (Cat# TP308675). The protein was produced from HEK293T cells transfected with BYSL cDNA clone (Cat# RC208675) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BYSL
• Synonyms: BYSTIN; Enp1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208675 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCAAATTCAAGGCGGCCCGTGGGGTGGGGGGTCAGGAAAAACATGCGCCCCTGGCCGATCAGATCC
  TGGCTGGGAATGCGGTGCGGGCGGGGGTCCGGGAGAAGCGGCGGGGTCGCGGGACAGGAGAAGCGGAGGA
  AGAGTATGTGGGGCCCCGGCTGAGCCGACGGATTTTGCAGCAAGCACGGCAGCAACAGGAGGAACTCGAG
  GCCGAGCATGGGACTGGGGACAAGCCCGCGGCGCCGCGGGAACGCACCACGCGGCTGGGTCCAAGAATGC
  CTCAGGATGGATCAGATGACGAGGACGAGGAGTGGCCCACCCTGGAGAAGGCTGCCACAATGACAGCAGC
  GGGCCATCATGCAGAGGTGGTTGTGGACCCTGAGGATGAGCGTGCCATAGAGATGTTCATGAACAAGAAC
  CCTCCTGCCAGGCGCACCCTGGCTGACATCATCATGGAGAAGCTGACTGAGAAGCAGACAGAGGTTGAGA
  CAGTCATGTCAGAGGTGTCGGGCTTCCCTATGCCCCAGCTGGACCCCCGGGTCCTAGAAGTGTACAGGGG
  GGTCCGGGAGGTATTATCTAAGTACCGCAGTGGAAAACTGCCCAAGGCATTTAAGATCATCCCTGCACTC
  TCCAACTGGGAGCAAATCCTCTACGTCACAGAGCCGGAGGCCTGGACTGCAGCTGCCATGTACCAGGCCA
  CCAGGATTTTTGCCTCTAACCTGAAGGAACGCATGGCCCAGCGCTTCTACAACCTTGTCCTGCTCCCTCG
  AGTACGAGATGACGTTGCTGAATACAAACGACTCAACTTCCATCTCTACATGGCTCTCAAGAAGGCCCTT
  TTCAAACCTGGAGCCTGGTTCAAAGGGATCCTGATTCCACTGTGCGAGTCTGGCACTTGTACCCTCCGGG
  AAGCCATCATTGTGGGTAGCATCATCACCAAGTGCTCCATCCCTGTGTTGCACTCCAGTGCGGCCATGCT
  GAAAATTGCTGAGATGGAATACAGCGGTGCCAACAGCATCTTCCTGCGACTGCTGCTGGATAAGAAGTAT
  GCACTGCCTTACCGGGTGCTGGATGCCCTAGTCTTCCACTTCCTGGGGTTCCGGACAGAGAAGCGTGAAC
  TGCCTGTGCTGTGGCACCAGTGCCTCCTGACTTTGGTCCAGCGCTACAAGGCCGACTTGGCCACAGACCA
  GAAAGAGGCCCTCTTAGAACTGCTCCGGCTGCAGCCCCATCCACAGCTATCGCCCGAAATCAGGCGTGAG
  CTTCAGAGTGCAGTCCCCCGCGATGTGGAAGATGTTCCCATCACCGTGGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208675 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPKFKAARGVGGQEKHAPLADQILAGNAVRAGVREKRRGRGTGEAEEEYVGPRLSRRILQQARQQQEELE
  AEHGTGDKPAAPRERTTRLGPRMPQDGSDDEDEEWPTLEKAATMTAAGHHAEVVVDPEDERAIEMFMNKN
  PPARRTLADIIMEKLTEKQTEVETVMSEVSGFPMPQLDPRVLEVYRGVREVLSKYRSGKLPKAFKIIPAL
  SNWEQILYVTEPEAWTAAAMYQATRIFASNLKERMAQRFYNLVLLPRVRDDVAEYKRLNFHLYMALKKAL
  FKPGAWFKGILIPLCESGTCTLREAIIVGSIITKCSIPVLHSSAAMLKIAEMEYSGANSIFLRLLLDKKY
  ALPYRVLDALVFHFLGFRTEKRELPVLWHQCLLTLVQRYKADLATDQKEALLELLRLQPHPQLSPEIRRE
  LQSAVPRDVEDVPITVE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004053
• ORF Size: 1311 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004053.4
• RefSeq Size: 2005 bp
• RefSeq ORF: 1314 bp
• Locus ID: 705
• UniProt ID: Q13895
• Cytogenetics: 6p21.1
• Protein Families: Stem cell - Pluripotency
• MW: 49.6 kDa
• Gene Summary: Bystin is expressed as a 2-kb major transcript and a 3.6-kb minor transcript in SNG-M cells and in human trophoblastic teratocarcinoma HT-H cells. Protein binding assays determined that bystin binds directly to trophinin and tastin, and that binding is enhanced when cytokeratins 8 and 18 are present. Immunocytochemistry of HT-H cells showed that bystin colocalizes with trophinin, tastin, and the cytokeratins, suggesting that these molecules form a complex in trophectoderm cells at the time of implantation. Using immunohistochemistry it was determined that trophinin and bystin are found in the placenta from the sixth week of pregnancy. Both proteins were localized in the cytoplasm of the syncytiotrophoblast in the chorionic villi and in endometrial decidual cells at the uteroplacental interface. After week 10, the levels of trophinin, tastin, and bystin decreased and then disappeared from placental villi. [provided by RefSeq, Jul 2008]