CPEB1 (NM_030594) Human Tagged ORF Clone

CAT#: RC208662

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CPEB1 (Myc-DDK-tagged)-Human cytoplasmic polyadenylation element binding protein 1 (CPEB1), transcript variant 1



  "NM_030594" in other vectors (6)

Reconstitution Protocol

USD 522.00


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Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol CPEB1
Synonyms CPE-BP1; CPEB; CPEB-1; h-CPEB; hCPEB-1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC208662 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGTTCCCGCTGGAAGAAGAAGCAGGAAGGATAAAAGATTGCTGGGACAACCAGGAAGCACCTGCTC
TCTCCACGTGTAGTAATGCCAATATCTTTCGAAGGATAAATGCCATATTGGATAATTCTCTGGATTTCAG
TAGAGTCTGCACTACACCTATAAACCGAGGAATTCATGATCATTTGCCAGACTTCCAGGACTCTGAAGAA
ACAGTTACAAGCAGGATGCTTTTCCCAACCTCTGCGCAAGAATCTTCCCGTGGCCTCCCAGATGCAAATG
ACTTGTGCCTTGGCCTGCAGTCCCTCAGTCTGACAGGCTGGGACCGACCCTGGAGCACCCAGGACTCAGA
TTCCTCAGCCCAGAGCAGCACACACTCGGTACTGAGCATGCTCCATAACCCACTGGGAAATGTCCTAGGA
AAACCCCCCTTGAGCTTCCTGCCTCTGGATCCCCTTGGGTCTGACTTGGTGGACAAGTTTCCAGCACCCT
CAGTTAGAGGATCACGCCTGGACACCCGGCCCATCCTGGACTCTCGATCTAGCAGCCCCTCTGACTCAGA
CACCAGTGGCTTCAGCTCTGGATCAGATCATCTCTCAGATTTGATTTCAAGCCTTCGCATTTCTCCACCT
CTGCCCTTCCTGTCTCTGTCAGGGGGTGGTCCCAGAGACCCTTTAAAGATGGGGGTAGGGTCTCGGATGG
ACCAAGAGCAAGCTGCTCTTGCTGCAGTCACTCCCTCCCCAACCAGTGCTTCAAAGAGATGGCCAGGAGC
TTCTGTGTGGCCATCCTGGGACCTCCTCGAAGCTCCCAAAGACCCCTTCAGCATAGAGAGAGAGGCCAGG
CTGCACCGACAAGCTGCAGCTGTGAATGAAGCCACCTGTACCTGGAGTGGCCAGCTTCCTCCCCGGAACT
ATAAGAACCCCATCTACTCTTGCAAGGTGTTTCTAGGAGGTGTTCCTTGGGATATTACAGAAGCTGGATT
AGTTAACACCTTCCGTGTTTTTGGCTCTTTGAGTGTGGAGTGGCCTGGTAAGGATGGCAAGCATCCCCGG
TGTCCTCCCAAAGGGTATGTGTATCTGGTCTTCGAACTAGAGAAGTCTGTCCGATCCTTGCTTCAGGCTT
GCTCTCATGACCCGCTGAGCCCAGATGGCCTGAGTGAATATTATTTCAAGATGTCCAGCCGAAGGATGCG
CTGCAAGGAGGTGCAGGTGATCCCCTGGGTATTAGCCGACAGTAACTTTGTCCGGAGCCCATCTCAGAGG
CTTGACCCCAGCAGGACGGTGTTTGTCGGTGCTCTGCATGGAATGCTAAATGCTGAGGCCCTGGCAGCCA
TCTTGAACGACCTATTTGGTGGAGTGGTGTATGCCGGGATTGACACAGATAAGCACAAGTATCCCATTGG
TTCTGGTCGTGTGACTTTCAATAACCAACGGAGTTACCTGAAAGCAGTCAGCGCTGCTTTTGTGGAGATC
AAAACCACCAAGTTCACAAAGAAGGTTCAGATTGACCCCTACCTAGAAGATTCTCTGTGTCATATCTGCA
GTTCTCAGCCTGGTCCTTTCTTCTGTCGAGATCAGGTCTGCTTCAAATACTTCTGCCGGAGCTGCTGGCA
CTGGCGGCACAGCATGGAGGGCCTGCGCCACCACAGCCCCCTGATGCGGAACCAGAAGAACCGAGATTCC
AGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC208662 protein sequence
Red=Cloning site Green=Tags(s)

MAFPLEEEAGRIKDCWDNQEAPALSTCSNANIFRRINAILDNSLDFSRVCTTPINRGIHDHLPDFQDSEE
TVTSRMLFPTSAQESSRGLPDANDLCLGLQSLSLTGWDRPWSTQDSDSSAQSSTHSVLSMLHNPLGNVLG
KPPLSFLPLDPLGSDLVDKFPAPSVRGSRLDTRPILDSRSSSPSDSDTSGFSSGSDHLSDLISSLRISPP
LPFLSLSGGGPRDPLKMGVGSRMDQEQAALAAVTPSPTSASKRWPGASVWPSWDLLEAPKDPFSIEREAR
LHRQAAAVNEATCTWSGQLPPRNYKNPIYSCKVFLGGVPWDITEAGLVNTFRVFGSLSVEWPGKDGKHPR
CPPKGYVYLVFELEKSVRSLLQACSHDPLSPDGLSEYYFKMSSRRMRCKEVQVIPWVLADSNFVRSPSQR
LDPSRTVFVGALHGMLNAEALAAILNDLFGGVVYAGIDTDKHKYPIGSGRVTFNNQRSYLKAVSAAFVEI
KTTKFTKKVQIDPYLEDSLCHICSSQPGPFFCRDQVCFKYFCRSCWHWRHSMEGLRHHSPLMRNQKNRDS
S

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_030594
ORF Size 1683 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_030594.5
RefSeq Size 3241 bp
RefSeq ORF 1686 bp
Locus ID 64506
UniProt ID Q9BZB8
Cytogenetics 15q25.2
Protein Pathways Dorso-ventral axis formation, Oocyte meiosis, Progesterone-mediated oocyte maturation
MW 62.1 kDa
Gene Summary This gene encodes a member of the cytoplasmic polyadenylation element binding protein family. This highly conserved protein binds to a specific RNA sequence, called the cytoplasmic polyadenylation element, found in the 3' untranslated region of some mRNAs. The encoded protein functions in both the cytoplasm and the nucleus. It is involved in the regulation of mRNA translation, as well as processing of the 3' untranslated region, and may play a role in cell proliferation and tumorigenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

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