CPEB1 (NM_030594) Human Tagged ORF Clone

CAT#: RC208662

CPEB1 (Myc-DDK-tagged)-Human cytoplasmic polyadenylation element binding protein 1 (CPEB1), transcript variant 1

Product Images

Circular map for RC208662

• 

  Western blot validation of overexpression lysate (Cat# LY410792) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208662 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CPEB1
• Synonyms: CPE-BP1; CPEB; CPEB-1; h-CPEB; hCPEB-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208662 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTTCCCGCTGGAAGAAGAAGCAGGAAGGATAAAAGATTGCTGGGACAACCAGGAAGCACCTGCTC
  TCTCCACGTGTAGTAATGCCAATATCTTTCGAAGGATAAATGCCATATTGGATAATTCTCTGGATTTCAG
  TAGAGTCTGCACTACACCTATAAACCGAGGAATTCATGATCATTTGCCAGACTTCCAGGACTCTGAAGAA
  ACAGTTACAAGCAGGATGCTTTTCCCAACCTCTGCGCAAGAATCTTCCCGTGGCCTCCCAGATGCAAATG
  ACTTGTGCCTTGGCCTGCAGTCCCTCAGTCTGACAGGCTGGGACCGACCCTGGAGCACCCAGGACTCAGA
  TTCCTCAGCCCAGAGCAGCACACACTCGGTACTGAGCATGCTCCATAACCCACTGGGAAATGTCCTAGGA
  AAACCCCCCTTGAGCTTCCTGCCTCTGGATCCCCTTGGGTCTGACTTGGTGGACAAGTTTCCAGCACCCT
  CAGTTAGAGGATCACGCCTGGACACCCGGCCCATCCTGGACTCTCGATCTAGCAGCCCCTCTGACTCAGA
  CACCAGTGGCTTCAGCTCTGGATCAGATCATCTCTCAGATTTGATTTCAAGCCTTCGCATTTCTCCACCT
  CTGCCCTTCCTGTCTCTGTCAGGGGGTGGTCCCAGAGACCCTTTAAAGATGGGGGTAGGGTCTCGGATGG
  ACCAAGAGCAAGCTGCTCTTGCTGCAGTCACTCCCTCCCCAACCAGTGCTTCAAAGAGATGGCCAGGAGC
  TTCTGTGTGGCCATCCTGGGACCTCCTCGAAGCTCCCAAAGACCCCTTCAGCATAGAGAGAGAGGCCAGG
  CTGCACCGACAAGCTGCAGCTGTGAATGAAGCCACCTGTACCTGGAGTGGCCAGCTTCCTCCCCGGAACT
  ATAAGAACCCCATCTACTCTTGCAAGGTGTTTCTAGGAGGTGTTCCTTGGGATATTACAGAAGCTGGATT
  AGTTAACACCTTCCGTGTTTTTGGCTCTTTGAGTGTGGAGTGGCCTGGTAAGGATGGCAAGCATCCCCGG
  TGTCCTCCCAAAGGGTATGTGTATCTGGTCTTCGAACTAGAGAAGTCTGTCCGATCCTTGCTTCAGGCTT
  GCTCTCATGACCCGCTGAGCCCAGATGGCCTGAGTGAATATTATTTCAAGATGTCCAGCCGAAGGATGCG
  CTGCAAGGAGGTGCAGGTGATCCCCTGGGTATTAGCCGACAGTAACTTTGTCCGGAGCCCATCTCAGAGG
  CTTGACCCCAGCAGGACGGTGTTTGTCGGTGCTCTGCATGGAATGCTAAATGCTGAGGCCCTGGCAGCCA
  TCTTGAACGACCTATTTGGTGGAGTGGTGTATGCCGGGATTGACACAGATAAGCACAAGTATCCCATTGG
  TTCTGGTCGTGTGACTTTCAATAACCAACGGAGTTACCTGAAAGCAGTCAGCGCTGCTTTTGTGGAGATC
  AAAACCACCAAGTTCACAAAGAAGGTTCAGATTGACCCCTACCTAGAAGATTCTCTGTGTCATATCTGCA
  GTTCTCAGCCTGGTCCTTTCTTCTGTCGAGATCAGGTCTGCTTCAAATACTTCTGCCGGAGCTGCTGGCA
  CTGGCGGCACAGCATGGAGGGCCTGCGCCACCACAGCCCCCTGATGCGGAACCAGAAGAACCGAGATTCC
  AGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208662 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAFPLEEEAGRIKDCWDNQEAPALSTCSNANIFRRINAILDNSLDFSRVCTTPINRGIHDHLPDFQDSEE
  TVTSRMLFPTSAQESSRGLPDANDLCLGLQSLSLTGWDRPWSTQDSDSSAQSSTHSVLSMLHNPLGNVLG
  KPPLSFLPLDPLGSDLVDKFPAPSVRGSRLDTRPILDSRSSSPSDSDTSGFSSGSDHLSDLISSLRISPP
  LPFLSLSGGGPRDPLKMGVGSRMDQEQAALAAVTPSPTSASKRWPGASVWPSWDLLEAPKDPFSIEREAR
  LHRQAAAVNEATCTWSGQLPPRNYKNPIYSCKVFLGGVPWDITEAGLVNTFRVFGSLSVEWPGKDGKHPR
  CPPKGYVYLVFELEKSVRSLLQACSHDPLSPDGLSEYYFKMSSRRMRCKEVQVIPWVLADSNFVRSPSQR
  LDPSRTVFVGALHGMLNAEALAAILNDLFGGVVYAGIDTDKHKYPIGSGRVTFNNQRSYLKAVSAAFVEI
  KTTKFTKKVQIDPYLEDSLCHICSSQPGPFFCRDQVCFKYFCRSCWHWRHSMEGLRHHSPLMRNQKNRDS
  S
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_030594
• ORF Size: 1683 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030594.5
• RefSeq Size: 3241 bp
• RefSeq ORF: 1686 bp
• Locus ID: 64506
• UniProt ID: Q9BZB8
• Cytogenetics: 15q25.2
• Protein Pathways: Dorso-ventral axis formation, Oocyte meiosis, Progesterone-mediated oocyte maturation
• MW: 62.1 kDa
• Gene Summary: This gene encodes a member of the cytoplasmic polyadenylation element binding protein family. This highly conserved protein binds to a specific RNA sequence, called the cytoplasmic polyadenylation element, found in the 3' untranslated region of some mRNAs. The encoded protein functions in both the cytoplasm and the nucleus. It is involved in the regulation of mRNA translation, as well as processing of the 3' untranslated region, and may play a role in cell proliferation and tumorigenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]