GPC1 (NM_002081) Human Tagged ORF Clone

CAT#: RC208602

GPC1 (Myc-DDK-tagged)-Human glypican 1 (GPC1)

Product Images

Circular map for RC208602

• 

  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY GPC1 (Cat# RC208602, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GPC1 (Cat# TA812439)(1:500).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GPC1
• Synonyms: glypican
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208602 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCTCCGGGCCCGAGGCTGGTGGCTGCTATGTGCGGCCGCAGCGCTGGTCGCCTGCGCCCGCGGGG
  ACCCGGCCAGCAAGAGCCGGAGCTGCGGCGAGGTCCGCCAGATCTACGGAGCCAAGGGCTTCAGCCTGAG
  CGACGTGCCCCAGGCGGAGATCTCGGGTGAGCACCTGCGGATCTGTCCCCAGGGCTACACCTGCTGCACC
  AGCGAGATGGAGGAGAACCTGGCCAACCGCAGCCATGCCGAGCTGGAGACCGCGCTCCGGGACAGCAGCC
  GCGTCCTGCAGGCCATGCTTGCCACCCAGCTGCGCAGCTTCGATGACCACTTCCAGCACCTGCTGAACGA
  CTCGGAGCGGACGCTGCAGGCCACCTTCCCCGGCGCCTTCGGAGAGCTGTACACGCAGAACGCGAGGGCC
  TTCCGGGACCTGTACTCAGAGCTGCGCCTGTACTACCGCGGTGCCAACCTGCACCTGGAGGAGACGCTGG
  CCGAGTTCTGGGCCCGCCTGCTCGAGCGCCTCTTCAAGCAGCTGCACCCCCAGCTGCTGCTGCCTGATGA
  CTACCTGGACTGCCTGGGCAAGCAGGCCGAGGCGCTGCGGCCCTTCGGGGAGGCCCCGAGAGAGCTGCGC
  CTGCGGGCCACCCGTGCCTTCGTGGCTGCTCGCTCCTTTGTGCAGGGCCTGGGCGTGGCCAGCGACGTGG
  TCCGGAAAGTGGCTCAGGTCCCCCTGGGCCCGGAGTGCTCGAGAGCTGTCATGAAGCTGGTCTACTGTGC
  TCACTGCCTGGGAGTCCCCGGCGCCAGGCCCTGCCCTGACTATTGCCGAAATGTGCTCAAGGGCTGCCTT
  GCCAACCAGGCCGACCTGGACGCCGAGTGGAGGAACCTCCTGGACTCCATGGTGCTCATCACCGACAAGT
  TCTGGGGTACATCGGGTGTGGAGAGTGTCATCGGCAGCGTGCACACGTGGCTGGCGGAGGCCATCAACGC
  CCTCCAGGACAACAGGGACACGCTCACGGCCAAGGTCATCCAGGGCTGCGGGAACCCCAAGGTCAACCCC
  CAGGGCCCTGGGCCTGAGGAGAAGCGGCGCCGGGGCAAGCTGGCCCCGCGGGAGAGGCCACCTTCAGGCA
  CGCTGGAGAAGCTGGTCTCTGAAGCCAAGGCCCAGCTCCGCGACGTCCAGGACTTCTGGATCAGCCTCCC
  AGGGACACTGTGCAGTGAGAAGATGGCCCTGAGCACTGCCAGTGATGACCGCTGCTGGAACGGGATGGCC
  AGAGGCCGGTACCTCCCCGAGGTCATGGGTGACGGCCTGGCCAACCAGATCAACAACCCCGAGGTGGAGG
  TGGACATCACCAAGCCGGACATGACCATCCGGCAGCAGATCATGCAGCTGAAGATCATGACCAACCGGCT
  GCGCAGCGCCTACAACGGCAACGACGTGGACTTCCAGGACGCCAGTGACGACGGCAGCGGCTCGGGCAGC
  GGTGATGGCTGTCTGGATGACCTCTGCGGCCGGAAGGTCAGCAGGAAGAGCTCCAGCTCCCGGACGCCCT
  TGACCCATGCCCTCCCAGGCCTGTCAGAGCAGGAAGGACAGAAGACCTCGGCTGCCAGCTGCCCCCAGCC
  CCCGACCTTCCTCCTGCCCCTCCTCCTCTTCCTGGCCCTTACAGTAGCCAGGCCCCGGTGGCGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208602 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MELRARGWWLLCAAAALVACARGDPASKSRSCGEVRQIYGAKGFSLSDVPQAEISGEHLRICPQGYTCCT
  SEMEENLANRSHAELETALRDSSRVLQAMLATQLRSFDDHFQHLLNDSERTLQATFPGAFGELYTQNARA
  FRDLYSELRLYYRGANLHLEETLAEFWARLLERLFKQLHPQLLLPDDYLDCLGKQAEALRPFGEAPRELR
  LRATRAFVAARSFVQGLGVASDVVRKVAQVPLGPECSRAVMKLVYCAHCLGVPGARPCPDYCRNVLKGCL
  ANQADLDAEWRNLLDSMVLITDKFWGTSGVESVIGSVHTWLAEAINALQDNRDTLTAKVIQGCGNPKVNP
  QGPGPEEKRRRGKLAPRERPPSGTLEKLVSEAKAQLRDVQDFWISLPGTLCSEKMALSTASDDRCWNGMA
  RGRYLPEVMGDGLANQINNPEVEVDITKPDMTIRQQIMQLKIMTNRLRSAYNGNDVDFQDASDDGSGSGS
  GDGCLDDLCGRKVSRKSSSSRTPLTHALPGLSEQEGQKTSAASCPQPPTFLLPLLLFLALTVARPRWR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002081
• ORF Size: 1674 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002081.1, NP_002072.1
• RefSeq Size: 3686 bp
• RefSeq ORF: 1677 bp
• Locus ID: 2817
• UniProt ID: P35052
• Cytogenetics: 2q37.3
• Domains: Glypican
• Protein Families: Druggable Genome
• MW: 61.7 kDa
• Gene Summary: Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. [provided by RefSeq, Jul 2008]