ZYG11BL (ZER1) (NM_006336) Human Tagged ORF Clone

CAT#: RC208590

ZER1 (Myc-DDK-tagged)-Human zer-1 homolog (C. elegans) (ZER1)

Product Images

Circular map for RC208590

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  Western blot validation of overexpression lysate (Cat# LY416723) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208590 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ZER1 protein (Cat# TP308590). The protein was produced from HEK293T cells transfected with ZER1 cDNA clone (Cat# RC208590) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZYG11BL
• Synonyms: C9orf60; ZYG; ZYG11BL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208590 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTCCGACACTCCCGAGTCGCTGATGGCCCTCTGTACTGACTTCTGCTTGCGCAACCTGGATGGCA
  CCCTGGGCTACCTGCTGGACAAGGAGACCCTGCGGCTACATCCGGACATCTTCTTGCCCAGCGAGATCTG
  TGACCGGCTCGTCAATGAGTATGTGGAGCTGGTGAACGCTGCCTGTAACTTCGAGCCACACGAGAGCTTC
  TTCAGCCTCTTTTCGGACCCCCGCAGCACCCGCCTCACGCGGATCCACCTCCGTGAGGACCTGGTGCAGG
  ACCAGGACCTGGAGGCCATCCGCAAGCAGGACCTGGTGGAGCTGTACCTGACTAACTGCGAGAAGCTGTC
  CGCCAAGAGCCTGCAGACACTGAGGAGCTTCAGCCACACCCTGGTGTCCTTGAGCCTCTTCGGCTGTACA
  AACATTTTCTATGAGGAGGAGAACCCAGGGGGCTGTGAAGATGAGTACCTCGTCAACCCCACCTGCCAGG
  TGCTGGTTAAGGATTTCACCTTCGAGGGCTTCAGCCGCCTCCGCTTCCTCAACTTGGGCCGCATGATTGA
  TTGGGTCCCTGTGGAGTCCCTGCTGCGGCCGCTTAACTCCCTGGCTGCCTTGGACCTCTCAGGCATTCAG
  ACGAGCGACGCCGCCTTCCTCACCCAGTGGAAAGACAGCCTGGTGTCCCTCGTCCTCTACAACATGGACC
  TGTCCGACGACCACATCCGGGTCATCGTGCAGCTGCACAAGCTGCGACACCTGGACATCTCCCGAGACCG
  CCTCTCCAGCTACTACAAGTTCAAGCTGACTCGGGAGGTGCTGAGCCTCTTTGTGCAGAAGCTGGGGAAC
  CTAATGTCCCTGGACATCTCTGGCCACATGATCCTAGAGAACTGCAGCATCTCCAAGATGGAAGAGGAAG
  CGGGGCAGACCAGCATTGAGCCTTCCAAGAGCAGCATCATACCTTTCCGGGCTCTGAAGAGGCCGCTGCA
  GTTCCTCGGGCTCTTTGAGAACTCTCTGTGCCGCCTCACGCACATTCCAGCCTACAAAGTAAGTGGTGAC
  AAAAACGAAGAGCAGGTGCTGAATGCCATCGAGGCCTACACGGAGCACCGGCCTGAGATCACCTCGCGGG
  CCATCAACTTGCTTTTTGACATCGCCCGCATCGAGCGTTGCAACCAGCTGCTGCGGGCCCTGAAGCTGGT
  CATCACGGCCCTCAAGTGCCACAAATATGACAGGAACATTCAAGTGACAGGCAGCGCCGCTCTCTTCTAC
  CTAACAAATTCCGAGTACCGCTCAGAGCAGAGTGTGAAGCTGCGCCGGCAGGTTATCCAGGTGGTGCTGA
  ATGGCATGGAATCCTACCAGGAGGTGACGGTGCAGCGGAACTGCTGCCTGACGCTCTGCAACTTCAGCAT
  CCCCGAGGAGCTGGAATTCCAGTACCGCCGGGTCAACGAGCTCCTGCTCAGCATCCTCAACCCCACGCGG
  CAGGACGAGTCTATCCAGCGGATCGCCGTGCACCTGTGCAATGCCCTGGTCTGCCAGGTAGACAACGACC
  ACAAGGAGGCCGTGGGCAAGATGGGCTTTGTCGTGACCATGCTGAAGCTGATTCAGAAGAAGCTGCTGGA
  CAAGACATGTGACCAGGTCATGGAGTTCTCCTGGAGTGCCCTGTGGAACATCACAGATGAAACTCCTGAC
  AACTGCGAGATGTTCCTCAATTTCAACGGCATGAAGCTCTTCCTGGACTGCCTGAAGGAATTCCCAGAGA
  AGCAGGAACTGCATAGGAATATGCTAGGACTTTTGGGGAATGTGGCAGAAGTGAAGGAGCTGAGGCCTCA
  ACTAATGACTTCCCAGTTCATCAGCGTCTTCAGCAACCTGTTGGAGAGCAAGGCCGATGGGATCGAGGTT
  TCCTACAATGCCTGCGGCGTCCTCTCCCACATCATGTTTGATGGACCCGAGGCCTGGGGCGTCTGTGAGC
  CCCAGCGTGAGGAGGTGGAGGAACGCATGTGGGCTGCCATCCAGAGCTGGGACATAAACTCTCGGAGAAA
  CATCAATTACAGGTCATTTGAACCAATTCTCCGCCTCCTTCCCCAGGGAATCTCTCCTGTCAGCCAGCAC
  TGGGCAACCTGGGCCCTGTATAACCTCGTGTCTGTCTACCCGGACAAGTACTGCCCTCTGCTGATCAAAG
  AAGGGGGGATGCCCCTTCTGAGGGACATAATTAAGATGGCGACCGCACGGCAGGAGACCAAGGAAATGGC
  CCGCAAGGTGATTGAGCACTGCAGTAACTTTAAAGAGGAGAACATGGACACGTCTAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208590 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MASDTPESLMALCTDFCLRNLDGTLGYLLDKETLRLHPDIFLPSEICDRLVNEYVELVNAACNFEPHESF
  FSLFSDPRSTRLTRIHLREDLVQDQDLEAIRKQDLVELYLTNCEKLSAKSLQTLRSFSHTLVSLSLFGCT
  NIFYEEENPGGCEDEYLVNPTCQVLVKDFTFEGFSRLRFLNLGRMIDWVPVESLLRPLNSLAALDLSGIQ
  TSDAAFLTQWKDSLVSLVLYNMDLSDDHIRVIVQLHKLRHLDISRDRLSSYYKFKLTREVLSLFVQKLGN
  LMSLDISGHMILENCSISKMEEEAGQTSIEPSKSSIIPFRALKRPLQFLGLFENSLCRLTHIPAYKVSGD
  KNEEQVLNAIEAYTEHRPEITSRAINLLFDIARIERCNQLLRALKLVITALKCHKYDRNIQVTGSAALFY
  LTNSEYRSEQSVKLRRQVIQVVLNGMESYQEVTVQRNCCLTLCNFSIPEELEFQYRRVNELLLSILNPTR
  QDESIQRIAVHLCNALVCQVDNDHKEAVGKMGFVVTMLKLIQKKLLDKTCDQVMEFSWSALWNITDETPD
  NCEMFLNFNGMKLFLDCLKEFPEKQELHRNMLGLLGNVAEVKELRPQLMTSQFISVFSNLLESKADGIEV
  SYNACGVLSHIMFDGPEAWGVCEPQREEVEERMWAAIQSWDINSRRNINYRSFEPILRLLPQGISPVSQH
  WATWALYNLVSVYPDKYCPLLIKEGGMPLLRDIIKMATARQETKEMARKVIEHCSNFKEENMDTSR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006336
• ORF Size: 2298 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006336.4
• RefSeq Size: 4314 bp
• RefSeq ORF: 2301 bp
• Locus ID: 10444
• UniProt ID: Q7Z7L7
• Cytogenetics: 9q34.11
• Domains: Armadillo_seg
• MW: 88.2 kDa
• Gene Summary: This gene encodes a subunit of an E3 ubiquitin ligase complex that may be involved in meiosis. The encoded protein contains three leucine-rich repeat motifs. [provided by RefSeq, Nov 2012]