FTCD (NM_006657) Human Tagged ORF Clone

CAT#: RC208573

FTCD (Myc-DDK-tagged)-Human formiminotransferase cyclodeaminase (FTCD), transcript variant B

Product Images

Circular map for RC208573

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY FTCD (Cat# RC208573, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FTCD(Cat# TA505028). Positive lysates LY416500 (100ug) and LC416500 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY404121) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC220581 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified FTCD protein (Cat# TP308573). The protein was produced from HEK293T cells transfected with FTCD cDNA clone (Cat# RC208573) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FTCD
• Synonyms: LCHC1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208573 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCAGCTGGTGGAATGCGTCCCCAACTTTTCGGAGGGGAAGAACCAGGAGGTGATCGACGCCATCT
  CTGGAGCCATCACACAGACCCCGGGCTGCGTGCTGCTGGATGTGGACGCAGGCCCTTCCACCAACCGCAC
  CGTGTACACCTTCGTGGGGCCGCCGGAGTGCGTGGTGGAGGGGGCCCTCAACGCTGCCCGGGTAGCTTCC
  CGACTTATCGACATGAGCAGGCACCAAGGAGAGCACCCCCGCATGGGGGCCCTAGACGTCTGCCCCTTCA
  TCCCCGTGAGGGGCGTCAGCGTGGATGAGTGTGTGCTCTGCGCCCAGGCCTTTGGCCAGAGGCTGGCAGA
  GGAGCTGGACGTGCCAGTTTACCTGTACGGCGAGGCAGCCAGGATGGACAGTCGCCGGACCCTGCCGGCC
  ATCCGGGCCGGGGAGTACGAGGCCCTCCCTAAGAAGCTCCAGCAGGCCGACTGGGCGCCCGACTTTGGTC
  CCAGCTCCTTTGTCCCCAGTTGGGGGGCCACGGCCACGGGGGCGAGGAAGTTCCTCATTGCTTTTAACAT
  CAACCTGCTCGGCACAAAGGAGCAAGCCCACCGCATCGCGCTCAACCTGCGGGAGCAGGGCCGCGGGAAG
  GACCAGCCAGGACGTCTGAAGAAAGTTCAGGGCATTGGCTGGTACCTGGATGAGAAGAACCTGGCTCAGG
  TGTCCACCAATCTTCTGGACTTTGAGGTCACGGCACTGCACACGGTCTACGAGGAGACCTGCCGAGAAGC
  ACAGGAGCTGAGCCTCCCAGTGGTGGGCTCACAGCTGGTGGGCCTGGTGCCCCTGAAGGCTCTGCTGGAT
  GCGGCCGCCTTCTACTGCGAGAAGGAGAACCTCTTCATCCTGGAGGAGGAGCAGCGGATCAGGCTGGTGG
  TGAGCCGGCTGGGCCTGGACTCCCTGTGCCCCTTCAGCCCTAAGGAGCGGATCATCGAGTACCTGGTCCC
  TGAGCGCGGGCCTGAGCGAGGCCTGGGCAGCAAGTCCCTGCGCGCCTTCGTGGGGGAGGTGGGTGCCCGC
  TCTGCGGCCCCCGGGGGCGGCTCGGTGGCGGCGGCCGCTGCGGCCATGGGTGCGGCGCTGGGCTCCATGG
  TGGGCCTCATGACCTACGGGCGGCGCCAATTCCAGTCCCTGGACACGACGATGCGGCGCCTGATCCCGCC
  CTTCCGCGAGGCTTCGGCCAAGCTAACCACGCTGGTGGATGCCGACGCCGAGGCCTTCACCGCCTACCTG
  GAAGCAATGAGGCTCCCCAAGAACACACCTGAGGAAAAGGACAGGCGCACGGCGGCCCTACAGGAGGGTC
  TGAGGCGGGCAGTCTCTGTGCCGCTGACGCTGGCGGAGACGGTGGCCTCGCTGTGGCCGGCGCTGCAGGA
  ACTGGCCCGGTGTGGGAACCTGGCCTGCCGGTCAGACCTCCAGGTGGCGGCCAAAGCCCTGGAGATGGGC
  GTGTTTGGCGCATATTTCAACGTGCTCATCAACCTGAGGGACATCACAGACGAGGCATTTAAGGACCAGA
  TCCACCATCGTGTTTCCAGCCTCCTGCAGGAAGCCAAGACCCAGGCTGCACTGGTGCTGGACTGCTTGGA
  GACCCGGCAGGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208573 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSQLVECVPNFSEGKNQEVIDAISGAITQTPGCVLLDVDAGPSTNRTVYTFVGPPECVVEGALNAARVAS
  RLIDMSRHQGEHPRMGALDVCPFIPVRGVSVDECVLCAQAFGQRLAEELDVPVYLYGEAARMDSRRTLPA
  IRAGEYEALPKKLQQADWAPDFGPSSFVPSWGATATGARKFLIAFNINLLGTKEQAHRIALNLREQGRGK
  DQPGRLKKVQGIGWYLDEKNLAQVSTNLLDFEVTALHTVYEETCREAQELSLPVVGSQLVGLVPLKALLD
  AAAFYCEKENLFILEEEQRIRLVVSRLGLDSLCPFSPKERIIEYLVPERGPERGLGSKSLRAFVGEVGAR
  SAAPGGGSVAAAAAAMGAALGSMVGLMTYGRRQFQSLDTTMRRLIPPFREASAKLTTLVDADAEAFTAYL
  EAMRLPKNTPEEKDRRTAALQEGLRRAVSVPLTLAETVASLWPALQELARCGNLACRSDLQVAAKALEMG
  VFGAYFNVLINLRDITDEAFKDQIHHRVSSLLQEAKTQAALVLDCLETRQE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006657
• ORF Size: 1623 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006657.1
• RefSeq Size: 1921 bp
• RefSeq ORF: 1626 bp
• Locus ID: 10841
• UniProt ID: O95954
• Cytogenetics: 21q22.3
• Protein Pathways: Histidine metabolism, Metabolic pathways, One carbon pool by folate
• MW: 58.9 kDa
• Gene Summary: The protein encoded by this gene is a bifunctional enzyme that channels 1-carbon units from formiminoglutamate, a metabolite of the histidine degradation pathway, to the folate pool. Mutations in this gene are associated with glutamate formiminotransferase deficiency. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Dec 2009]