C9orf98 (AK8) (NM_152572) Human Tagged ORF Clone

CAT#: RC208540

AK8 (Myc-DDK-tagged)-Human adenylate kinase 8 (AK8)

Product Images

Circular map for RC208540

• 

  Western blot validation of overexpression lysate (Cat# LY407442) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208540 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: C9orf98
• Synonyms: AK 8; C9orf98; DDX31
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208540 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGCCACTATCGCCCCGCACCGTATCCCCCCCGAGATGCCCCAGTACGGGGAGGAGAACCACATCT
  TCGAGTTGATGCAGAACATGCTGGAGCAACTCCTGATCCACCAGCCCGAAGATCCCATCCCCTTCATGAT
  CCAGCACTTGCATAGAGACAACGACAATGTGCCCAGGATTGTAATATTAGGTCCACCCGCCTCAGGGAAA
  ACAACAATAGCAATGTGGCTCTGCAAACATCTGAACAGCAGTCTCCTCACCCTGGAGAACCTGATCTTAA
  ATGAGTTTTCCTATACGGCCACCGAAGCCAGAAGGCTTTATCTGCAAAGGAAGACAGTTCCCAGCGCGCT
  GCTCGTCCAGCTGATTCAGGAACGCCTGGCTGAAGAGGATTGCATCAAGCAGGGCTGGATTCTGGATGGC
  ATCCCTGAGACGCGTGAGCAGGCTCTGAGGATCCAGACCCTGGGGATCACACCCAGACACGTCATTGTGC
  TGAGTGCTCCAGACACGGTCCTGATCGAGAGAAACTTGGGGAAGAGAATCGACCCTCAAACTGGAGAGAT
  TTATCACACCACCTTTGACTGGCCACCCGAATCTGAAATCCAGAACCGTCTCATGGTGCCAGAGGACATC
  TCAGAGCTGGAGACGGCTCAGAAACTGCTGGAGTATCATAGGAACATCGTCAGGGTCATTCCCTCCTACC
  CCAAAATCCTCAAAGTCATCAGTGCTGACCAGCCATGTGTGGACGTCTTCTACCAGGCTCTGACCTATGT
  CCAAAGCAACCATCGTACTAATGCCCCGTTCACCCCGAGGGTGCTGCTGCTCGGGCCTGTGGGCAGTGGG
  AAAAGTCTGCAGGCCGCCCTCCTGGCCCAGAAATACAGGCTTGTCAATGTCTGCTGTGGGCAACTGCTGA
  AAGAGGCTGTGGCAGATAGGACCACGTTTGGCGAGCTCATCCAGCCCTTCTTTGAAAAGGAGATGGCAGT
  TCCTGACAGCCTCCTCATGAAGGTGCTGAGCCAGCGCCTGGACCAGCAGGACTGCATCCAGAAAGGCTGG
  GTGCTACACGGCGTCCCGCGGGACCTCGACCAGGCACACCTGCTGAACCGCCTGGGCTACAATCCCAACA
  GGGTGTTTTTCCTGAATGTGCCATTTGATTCCATCATGGAGCGGCTGACTCTGAGAAGAATTGATCCAGT
  CACTGGGGAAAGGTACCACCTCATGTACAAGCCACCTCCCACCATGGAGATCCAGGCTCGCCTCCTGCAG
  AACCCAAAGGATGCTGAAGAGCAGGTCAAGCTGAAAATGGACCTGTTCTACAGGAACTCAGCTGACTTGG
  AGCAGTTGTATGGGTCGGCCATCACCCTCAATGGGGACCAGGACCCATACACAGTCTTCGAATACATCGA
  GAGTGGGATCATTAATCCCCTGCCCAAGAAAATCCCC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208540 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDATIAPHRIPPEMPQYGEENHIFELMQNMLEQLLIHQPEDPIPFMIQHLHRDNDNVPRIVILGPPASGK
  TTIAMWLCKHLNSSLLTLENLILNEFSYTATEARRLYLQRKTVPSALLVQLIQERLAEEDCIKQGWILDG
  IPETREQALRIQTLGITPRHVIVLSAPDTVLIERNLGKRIDPQTGEIYHTTFDWPPESEIQNRLMVPEDI
  SELETAQKLLEYHRNIVRVIPSYPKILKVISADQPCVDVFYQALTYVQSNHRTNAPFTPRVLLLGPVGSG
  KSLQAALLAQKYRLVNVCCGQLLKEAVADRTTFGELIQPFFEKEMAVPDSLLMKVLSQRLDQQDCIQKGW
  VLHGVPRDLDQAHLLNRLGYNPNRVFFLNVPFDSIMERLTLRRIDPVTGERYHLMYKPPPTMEIQARLLQ
  NPKDAEEQVKLKMDLFYRNSADLEQLYGSAITLNGDQDPYTVFEYIESGIINPLPKKIP
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152572
• ORF Size: 1437 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152572.3
• RefSeq Size: 2185 bp
• RefSeq ORF: 1440 bp
• Locus ID: 158067
• UniProt ID: Q96MA6
• Cytogenetics: 9q34.13
• MW: 54.9 kDa
• Gene Summary: Nucleoside monophosphate (NMP) kinase that catalyzes the reversible transfer of the terminal phosphate group between nucleoside triphosphates and monophosphates. Has highest activity toward AMP, and weaker activity toward dAMP, CMP and dCMP. Also displays broad nucleoside diphosphate kinase activity.[UniProtKB/Swiss-Prot Function]