RAB5B (NM_002868) Human Tagged ORF Clone

CAT#: RC208529

RAB5B (Myc-DDK-tagged)-Human RAB5B, member RAS oncogene family (RAB5B)

Product Images

Western blot validation of overexpression lysate (Cat# LY419061) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208529 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RAB5B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208529 representing NM_002868
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTAGCAGAAGCACAGCTAGGCCCAATGGGCAACCCCAGGCCAGCAAAATTTGCCAGTTCAAATTGG
  TCCTGCTGGGAGAATCTGCAGTGGGAAAGTCAAGCCTGGTATTACGTTTTGTCAAAGGGCAGTTCCATGA
  GTACCAGGAGAGCACCATTGGAGCGGCCTTCCTCACCCAGTCCGTTTGTCTAGATGACACAACAGTGAAG
  TTTGAGATCTGGGACACAGCTGGGCAGGAGCGATATCACAGCTTAGCCCCCATGTACTACAGGGGTGCCC
  AAGCTGCAATCGTGGTTTACGACATTACTAATCAGGAAACCTTTGCCCGAGCAAAGACATGGGTGAAGGA
  ACTACAGCGACAGGCCAGTCCTAGCATCGTTATTGCCCTGGCAGGGAACAAAGCTGACCTGGCCAACAAA
  CGTATGGTGGAGTATGAAGAGGCCCAGGCATATGCAGATGACAACAGCTTATTGTTCATGGAGACTTCAG
  CCAAGACAGCTATGAACGTGAATGATCTCTTCCTGGCAATAGCTAAGAAGTTGCCAAAGAGTGAACCCCA
  GAATCTGGGAGGTGCAGCAGGCCGAAGCCGGGGTGTGGATCTCCATGAACAGTCCCAGCAGAACAAGAGC
  CAGTGTTGTAGCAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208529 representing NM_002868
  Red=Cloning site Green=Tags(s)
  
  MTSRSTARPNGQPQASKICQFKLVLLGESAVGKSSLVLRFVKGQFHEYQESTIGAAFLTQSVCLDDTTVK
  FEIWDTAGQERYHSLAPMYYRGAQAAIVVYDITNQETFARAKTWVKELQRQASPSIVIALAGNKADLANK
  RMVEYEEAQAYADDNSLLFMETSAKTAMNVNDLFLAIAKKLPKSEPQNLGGAAGRSRGVDLHEQSQQNKS
  QCCSN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002868
• ORF Size: 645 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002868.4
• RefSeq Size: 3336 bp
• RefSeq ORF: 648 bp
• Locus ID: 5869
• UniProt ID: P61020
• Cytogenetics: 12q13.2
• Domains: ras, RAN, RAS, RHO, RAB
• Protein Families: Druggable Genome
• Protein Pathways: Endocytosis
• MW: 23.5 kDa
• Gene Summary: Protein transport. Probably involved in vesicular traffic (By similarity).[UniProtKB/Swiss-Prot Function]