FARP1 (NM_005766) Human Tagged ORF Clone

CAT#: RC208329

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FARP1 (Myc-DDK-tagged)-Human FERM, RhoGEF (ARHGEF) and pleckstrin domain protein 1 (chondrocyte-derived) (FARP1), transcript variant 1



  "NM_005766" in other vectors (6)

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 1,247.00


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Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol FARP1
Synonyms CDEP; FARP1-IT1; PLEKHC2; PPP1R75
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC208329 representing NM_005766
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGAGAAATAGAGCAGAGGCCGACCCCAGGATCACGACTGGGGGCCCCGGAAAATTCGGGGATCAGTA
CCTTGGAACGTGGACAGAAGCCGCCCCCAACACCTTCAGGAAAACTCGTGTCCATCAAAATCCAGATGCT
GGATGACACCCAGGAGGCATTTGAAGTTCCACAAAGAGCTCCTGGGAAGGTGCTGCTGGATGCAGTTTGC
AACCACCTCAACCTCGTGGAAGGTGACTATTTTGGCCTCGAGTTTCCTGATCACAAAAAGATCACGGTGT
GGCTGGATCTCCTAAAACCCATTGTGAAACAGATTAGAAGGCCAAAGCACGTTGTTGTTAAGTTTGTGGT
GAAATTCTTTCCGCCTGACCACACACAACTCCAAGAAGAACTCACAAGGTACCTGTTCGCGCTGCAGGTG
AAGCAGGACTTGGCTCAAGGCAGGTTGACGTGTAATGACACCAGCGCAGCTCTCTTGATTTCACACATTG
TGCAATCTGAGATTGGGGATTTTGATGAAGCCTTGGACAGAGAGCACTTAGCAAAAAATAAATACATACC
TCAGCAAGACGCACTAGAGGACAAAATCGTGGAATTTCACCATAACCACATTGGACAAACACCAGCAGAA
TCAGATTTCCAGCTCCTAGAGATTGCCCGTCGGCTAGAGATGTATGGAATCCGGTTGCACCCGGCCAAGG
ACAGGGAAGGCACGAAGATCAATCTGGCCGTTGCCAACACGGGAATTCTAGTGTTTCAGGGTTTCACTAA
GATCAATGCCTTCAACTGGGCCAAGGTGCGGAAGCTGAGCTTCAAGAGGAAGCGCTTTCTCATCAAGCTC
CGGCCAGATGCCAATAGTGCGTACCAGGATACCTTGGAATTCCTGATGGCCAGTCGGGATTTCTGCAAGT
CCTTCTGGAAAATCTGTGTTGAACATCATGCCTTCTTTAGACTTTTTGAAGAGCCCAAACCAAAGCCCAA
GCCCGTCCTCTTTAGCCGGGGGTCATCATTTCGGTTCAGTGGTCGGACTCAGAAGCAGGTTCTCGACTAT
GTTAAAGAAGGAGGACATAAGAAGGTGCAGTTTGAAAGGAAGCACAGCAAGATTCATTCTATCCGGAGCC
TTGCTTCACAGCCTACAGAACTGAATTCGGAAGTGCTGGAGCAGTCTCAGCAGAGCACCAGCCTTACATT
TGGAGAAGGTGCCGAATCTCCAGGGGGCCAGAGCTGCCGGCGAGGAAAGGAACCGAAGGTTTCCGCCGGG
GAGCCGGGGTCGCACCCGAGCCCTGCGCCGAGGAGAAGCCCCGCGGGTAACAAGCAGGCGGACGGAGCCG
CCTCGGCGCCCACGGAGGAAGAGGAGGAGGTCGTTAAGGATAGGACCCAGCAGAGTAAACCTCAGCCCCC
GCAGCCAAGCACAGGCTCCCTGACTGGCAGTCCTCACCTTTCCGAGCTGTCTGTGAACTCGCAGGGGGGA
GTGGCCCCTGCCAACGTGACCTTGTCTCCCAACCTGAGCCCCGACACCAAGCAGGCCTCTCCCTTGATCA
GCCCGCTGCTGAATGACCAGGCCTGCCCCCGGACGGACGATGAGGATGAGGGCCGGAGGAAGAGATTCCC
AACTGATAAAGCGTACTTCATAGCTAAGGAAGTGTCTACCACCGAGCGAACATATCTGAAGGATCTCGAA
GTTATCACTTCGTGGTTTCAGAGCACAGTGAGCAAAGAGGACGCCATGCCGGAAGCACTGAAAAGTCTCA
TATTCCCGAATTTTGAACCTTTGCACAAATTTCATACTAATTTTCTCAAGGAAATTGAGCAACGACTTGC
CCTGTGGGAAGGCCGCTCAAATGCCCAAATCAGAGATTACCAAAGAATCGGCGATGTCATGCTGAAGAAC
ATTCAGGGCATGAAGCACCTGGCGGCTCACCTGTGGAAGCACAGCGAGGCCTTGGAGGCCCTGGAGAATG
GAATCAAGAGCTCCCGGCGGCTGGAGAACTTCTGCAGAGACTTTGAGCTGCAGAAGGTGTGTTACCTACC
GCTCAACACCTTCCTCCTGCGGCCACTGCACCGGCTCATGCACTACAAGCAGGTCCTGGAGCGGCTGTGC
AAACACCACCCGCCGAGCCACGCCGACTTCAGGGACTGCCGAGCCGCTTTGGCAGAGATCACGGAGATGG
TGGCACAGCTCCACGGTACGATGATCAAGATGGAGAATTTCCAGAAGCTGCACGAACTCAAGAAAGATTT
GATTGGCATTGACAATCTTGTGGTTCCGGGAAGGGAGTTCATCCGTCTGGGCAGCCTCAGCAAGCTCTCG
GGGAAGGGGCTCCAGCAGCGCATGTTCTTCCTGTTCAACGACGTCCTGCTATACACGAGCCGGGGGCTGA
CGGCCTCCAATCAGTTTAAAGTCCACGGGCAGCTCCCGCTCTATGGCATGACGATTGAGGAGAGCGAAGA
CGAGTGGGGGGTGCCCCACTGCCTGACCCTCCGGGGCCAGCGGCAGTCCATCATCGTGGCCGCCAGTTCT
CGGTCCGAGATGGAGAAGTGGGTTGAGGACATCCAGATGGCCATTGACCTGGCGGAGAAGAGCAGCAGCC
CCGCCCCTGAGTTCCTGGCCAGCAGCCCCCCTGACAACAAGTCCCCTGATGAAGCCACCGCGGCTGACCA
GGAGTCAGAGGATGACCTGAGCGCCTCGCGCACATCGCTGGAGCGCCAGGCCCCGCACCGCGGCAACACA
ATGGTGCACGTGTGCTGGCACCGCAACACCAGCGTCTCCATGGTGGACTTCAGCATCGCAGTGGAGAATC
AGTTGTCTGGAAACCTGCTGAGGAAATTCAAAAACAGCAACGGGTGGCAGAAGCTGTGGGTGGTGTTCAC
AAACTTCTGCCTGTTCTTCTACAAATCACACCAGGACAATCATCCCCTTGCCAGCCTGCCTCTGCTCGGC
TACTCGCTCACCATCCCCTCTGAGTCCGAGAACATCCAGAAAGACTACGTGTTCAAGCTGCACTTCAAGT
CCCACGTCTACTACTTCAGGGCGGAAAGCGAGTACACGTTCGAAAGGTGGATGGAAGTGATCCGCAGTGC
CACCAGCTCTGCCTCGCGACCCCACGTGTTGAGCCACAAAGAGTCTCTTGTGTAT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC208329 representing NM_005766
Red=Cloning site Green=Tags(s)

MGEIEQRPTPGSRLGAPENSGISTLERGQKPPPTPSGKLVSIKIQMLDDTQEAFEVPQRAPGKVLLDAVC
NHLNLVEGDYFGLEFPDHKKITVWLDLLKPIVKQIRRPKHVVVKFVVKFFPPDHTQLQEELTRYLFALQV
KQDLAQGRLTCNDTSAALLISHIVQSEIGDFDEALDREHLAKNKYIPQQDALEDKIVEFHHNHIGQTPAE
SDFQLLEIARRLEMYGIRLHPAKDREGTKINLAVANTGILVFQGFTKINAFNWAKVRKLSFKRKRFLIKL
RPDANSAYQDTLEFLMASRDFCKSFWKICVEHHAFFRLFEEPKPKPKPVLFSRGSSFRFSGRTQKQVLDY
VKEGGHKKVQFERKHSKIHSIRSLASQPTELNSEVLEQSQQSTSLTFGEGAESPGGQSCRRGKEPKVSAG
EPGSHPSPAPRRSPAGNKQADGAASAPTEEEEEVVKDRTQQSKPQPPQPSTGSLTGSPHLSELSVNSQGG
VAPANVTLSPNLSPDTKQASPLISPLLNDQACPRTDDEDEGRRKRFPTDKAYFIAKEVSTTERTYLKDLE
VITSWFQSTVSKEDAMPEALKSLIFPNFEPLHKFHTNFLKEIEQRLALWEGRSNAQIRDYQRIGDVMLKN
IQGMKHLAAHLWKHSEALEALENGIKSSRRLENFCRDFELQKVCYLPLNTFLLRPLHRLMHYKQVLERLC
KHHPPSHADFRDCRAALAEITEMVAQLHGTMIKMENFQKLHELKKDLIGIDNLVVPGREFIRLGSLSKLS
GKGLQQRMFFLFNDVLLYTSRGLTASNQFKVHGQLPLYGMTIEESEDEWGVPHCLTLRGQRQSIIVAASS
RSEMEKWVEDIQMAIDLAEKSSSPAPEFLASSPPDNKSPDEATAADQESEDDLSASRTSLERQAPHRGNT
MVHVCWHRNTSVSMVDFSIAVENQLSGNLLRKFKNSNGWQKLWVVFTNFCLFFYKSHQDNHPLASLPLLG
YSLTIPSESENIQKDYVFKLHFKSHVYYFRAESEYTFERWMEVIRSATSSASRPHVLSHKESLVY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_005766
ORF Size 3135 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_005766.1
RefSeq Size 5028 bp
RefSeq ORF 3138 bp
Locus ID 10160
UniProt ID Q9Y4F1
Cytogenetics 13q32.2
Domains RhoGEF, B41, PH
MW 118.5 kDa
Gene Summary This gene encodes a protein containing a FERM (4.2, exrin, radixin, moesin) domain, a Dbl homology domain, and two pleckstrin homology domains. These domains are found in guanine nucleotide exchange factors and proteins that link the cytoskeleton to the cell membrane. The encoded protein functions in neurons to promote dendritic growth. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2013]

Other Versions

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