FARP1 (NM_005766) Human Tagged ORF Clone

CAT#: RC208329

FARP1 (Myc-DDK-tagged)-Human FERM, RhoGEF (ARHGEF) and pleckstrin domain protein 1 (chondrocyte-derived) (FARP1), transcript variant 1

Product Images

Circular map for RC208329

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  Western blot validation of overexpression lysate (Cat# LY417090) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208329 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified FARP1 protein (Cat# TP308329). The protein was produced from HEK293T cells transfected with FARP1 cDNA clone (Cat# RC208329) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FARP1
• Synonyms: CDEP; FARP1-IT1; PLEKHC2; PPP1R75
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208329 representing NM_005766
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAGAAATAGAGCAGAGGCCGACCCCAGGATCACGACTGGGGGCCCCGGAAAATTCGGGGATCAGTA
  CCTTGGAACGTGGACAGAAGCCGCCCCCAACACCTTCAGGAAAACTCGTGTCCATCAAAATCCAGATGCT
  GGATGACACCCAGGAGGCATTTGAAGTTCCACAAAGAGCTCCTGGGAAGGTGCTGCTGGATGCAGTTTGC
  AACCACCTCAACCTCGTGGAAGGTGACTATTTTGGCCTCGAGTTTCCTGATCACAAAAAGATCACGGTGT
  GGCTGGATCTCCTAAAACCCATTGTGAAACAGATTAGAAGGCCAAAGCACGTTGTTGTTAAGTTTGTGGT
  GAAATTCTTTCCGCCTGACCACACACAACTCCAAGAAGAACTCACAAGGTACCTGTTCGCGCTGCAGGTG
  AAGCAGGACTTGGCTCAAGGCAGGTTGACGTGTAATGACACCAGCGCAGCTCTCTTGATTTCACACATTG
  TGCAATCTGAGATTGGGGATTTTGATGAAGCCTTGGACAGAGAGCACTTAGCAAAAAATAAATACATACC
  TCAGCAAGACGCACTAGAGGACAAAATCGTGGAATTTCACCATAACCACATTGGACAAACACCAGCAGAA
  TCAGATTTCCAGCTCCTAGAGATTGCCCGTCGGCTAGAGATGTATGGAATCCGGTTGCACCCGGCCAAGG
  ACAGGGAAGGCACGAAGATCAATCTGGCCGTTGCCAACACGGGAATTCTAGTGTTTCAGGGTTTCACTAA
  GATCAATGCCTTCAACTGGGCCAAGGTGCGGAAGCTGAGCTTCAAGAGGAAGCGCTTTCTCATCAAGCTC
  CGGCCAGATGCCAATAGTGCGTACCAGGATACCTTGGAATTCCTGATGGCCAGTCGGGATTTCTGCAAGT
  CCTTCTGGAAAATCTGTGTTGAACATCATGCCTTCTTTAGACTTTTTGAAGAGCCCAAACCAAAGCCCAA
  GCCCGTCCTCTTTAGCCGGGGGTCATCATTTCGGTTCAGTGGTCGGACTCAGAAGCAGGTTCTCGACTAT
  GTTAAAGAAGGAGGACATAAGAAGGTGCAGTTTGAAAGGAAGCACAGCAAGATTCATTCTATCCGGAGCC
  TTGCTTCACAGCCTACAGAACTGAATTCGGAAGTGCTGGAGCAGTCTCAGCAGAGCACCAGCCTTACATT
  TGGAGAAGGTGCCGAATCTCCAGGGGGCCAGAGCTGCCGGCGAGGAAAGGAACCGAAGGTTTCCGCCGGG
  GAGCCGGGGTCGCACCCGAGCCCTGCGCCGAGGAGAAGCCCCGCGGGTAACAAGCAGGCGGACGGAGCCG
  CCTCGGCGCCCACGGAGGAAGAGGAGGAGGTCGTTAAGGATAGGACCCAGCAGAGTAAACCTCAGCCCCC
  GCAGCCAAGCACAGGCTCCCTGACTGGCAGTCCTCACCTTTCCGAGCTGTCTGTGAACTCGCAGGGGGGA
  GTGGCCCCTGCCAACGTGACCTTGTCTCCCAACCTGAGCCCCGACACCAAGCAGGCCTCTCCCTTGATCA
  GCCCGCTGCTGAATGACCAGGCCTGCCCCCGGACGGACGATGAGGATGAGGGCCGGAGGAAGAGATTCCC
  AACTGATAAAGCGTACTTCATAGCTAAGGAAGTGTCTACCACCGAGCGAACATATCTGAAGGATCTCGAA
  GTTATCACTTCGTGGTTTCAGAGCACAGTGAGCAAAGAGGACGCCATGCCGGAAGCACTGAAAAGTCTCA
  TATTCCCGAATTTTGAACCTTTGCACAAATTTCATACTAATTTTCTCAAGGAAATTGAGCAACGACTTGC
  CCTGTGGGAAGGCCGCTCAAATGCCCAAATCAGAGATTACCAAAGAATCGGCGATGTCATGCTGAAGAAC
  ATTCAGGGCATGAAGCACCTGGCGGCTCACCTGTGGAAGCACAGCGAGGCCTTGGAGGCCCTGGAGAATG
  GAATCAAGAGCTCCCGGCGGCTGGAGAACTTCTGCAGAGACTTTGAGCTGCAGAAGGTGTGTTACCTACC
  GCTCAACACCTTCCTCCTGCGGCCACTGCACCGGCTCATGCACTACAAGCAGGTCCTGGAGCGGCTGTGC
  AAACACCACCCGCCGAGCCACGCCGACTTCAGGGACTGCCGAGCCGCTTTGGCAGAGATCACGGAGATGG
  TGGCACAGCTCCACGGTACGATGATCAAGATGGAGAATTTCCAGAAGCTGCACGAACTCAAGAAAGATTT
  GATTGGCATTGACAATCTTGTGGTTCCGGGAAGGGAGTTCATCCGTCTGGGCAGCCTCAGCAAGCTCTCG
  GGGAAGGGGCTCCAGCAGCGCATGTTCTTCCTGTTCAACGACGTCCTGCTATACACGAGCCGGGGGCTGA
  CGGCCTCCAATCAGTTTAAAGTCCACGGGCAGCTCCCGCTCTATGGCATGACGATTGAGGAGAGCGAAGA
  CGAGTGGGGGGTGCCCCACTGCCTGACCCTCCGGGGCCAGCGGCAGTCCATCATCGTGGCCGCCAGTTCT
  CGGTCCGAGATGGAGAAGTGGGTTGAGGACATCCAGATGGCCATTGACCTGGCGGAGAAGAGCAGCAGCC
  CCGCCCCTGAGTTCCTGGCCAGCAGCCCCCCTGACAACAAGTCCCCTGATGAAGCCACCGCGGCTGACCA
  GGAGTCAGAGGATGACCTGAGCGCCTCGCGCACATCGCTGGAGCGCCAGGCCCCGCACCGCGGCAACACA
  ATGGTGCACGTGTGCTGGCACCGCAACACCAGCGTCTCCATGGTGGACTTCAGCATCGCAGTGGAGAATC
  AGTTGTCTGGAAACCTGCTGAGGAAATTCAAAAACAGCAACGGGTGGCAGAAGCTGTGGGTGGTGTTCAC
  AAACTTCTGCCTGTTCTTCTACAAATCACACCAGGACAATCATCCCCTTGCCAGCCTGCCTCTGCTCGGC
  TACTCGCTCACCATCCCCTCTGAGTCCGAGAACATCCAGAAAGACTACGTGTTCAAGCTGCACTTCAAGT
  CCCACGTCTACTACTTCAGGGCGGAAAGCGAGTACACGTTCGAAAGGTGGATGGAAGTGATCCGCAGTGC
  CACCAGCTCTGCCTCGCGACCCCACGTGTTGAGCCACAAAGAGTCTCTTGTGTAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208329 representing NM_005766
  Red=Cloning site Green=Tags(s)
  
  MGEIEQRPTPGSRLGAPENSGISTLERGQKPPPTPSGKLVSIKIQMLDDTQEAFEVPQRAPGKVLLDAVC
  NHLNLVEGDYFGLEFPDHKKITVWLDLLKPIVKQIRRPKHVVVKFVVKFFPPDHTQLQEELTRYLFALQV
  KQDLAQGRLTCNDTSAALLISHIVQSEIGDFDEALDREHLAKNKYIPQQDALEDKIVEFHHNHIGQTPAE
  SDFQLLEIARRLEMYGIRLHPAKDREGTKINLAVANTGILVFQGFTKINAFNWAKVRKLSFKRKRFLIKL
  RPDANSAYQDTLEFLMASRDFCKSFWKICVEHHAFFRLFEEPKPKPKPVLFSRGSSFRFSGRTQKQVLDY
  VKEGGHKKVQFERKHSKIHSIRSLASQPTELNSEVLEQSQQSTSLTFGEGAESPGGQSCRRGKEPKVSAG
  EPGSHPSPAPRRSPAGNKQADGAASAPTEEEEEVVKDRTQQSKPQPPQPSTGSLTGSPHLSELSVNSQGG
  VAPANVTLSPNLSPDTKQASPLISPLLNDQACPRTDDEDEGRRKRFPTDKAYFIAKEVSTTERTYLKDLE
  VITSWFQSTVSKEDAMPEALKSLIFPNFEPLHKFHTNFLKEIEQRLALWEGRSNAQIRDYQRIGDVMLKN
  IQGMKHLAAHLWKHSEALEALENGIKSSRRLENFCRDFELQKVCYLPLNTFLLRPLHRLMHYKQVLERLC
  KHHPPSHADFRDCRAALAEITEMVAQLHGTMIKMENFQKLHELKKDLIGIDNLVVPGREFIRLGSLSKLS
  GKGLQQRMFFLFNDVLLYTSRGLTASNQFKVHGQLPLYGMTIEESEDEWGVPHCLTLRGQRQSIIVAASS
  RSEMEKWVEDIQMAIDLAEKSSSPAPEFLASSPPDNKSPDEATAADQESEDDLSASRTSLERQAPHRGNT
  MVHVCWHRNTSVSMVDFSIAVENQLSGNLLRKFKNSNGWQKLWVVFTNFCLFFYKSHQDNHPLASLPLLG
  YSLTIPSESENIQKDYVFKLHFKSHVYYFRAESEYTFERWMEVIRSATSSASRPHVLSHKESLVY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005766
• ORF Size: 3135 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005766.1
• RefSeq Size: 5028 bp
• RefSeq ORF: 3138 bp
• Locus ID: 10160
• UniProt ID: Q9Y4F1
• Cytogenetics: 13q32.2
• Domains: RhoGEF, B41, PH
• MW: 118.5 kDa
• Gene Summary: This gene encodes a protein containing a FERM (4.2, exrin, radixin, moesin) domain, a Dbl homology domain, and two pleckstrin homology domains. These domains are found in guanine nucleotide exchange factors and proteins that link the cytoskeleton to the cell membrane. The encoded protein functions in neurons to promote dendritic growth. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2013]