SARS2 (NM_017827) Human Tagged ORF Clone

CAT#: RC208306

SARS2 (Myc-DDK-tagged)-Human seryl-tRNA synthetase 2, mitochondrial (SARS2), nuclear gene encoding mitochondrial protein, transcript variant 2

Product Images

Circular map for RC208306

• 

  Western blot validation of overexpression lysate (Cat# LY413512) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208306 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified SARS2 protein (Cat# TP308306). The protein was produced from HEK293T cells transfected with SARS2 cDNA clone (Cat# RC208306) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SARS2
• Synonyms: mtSerRS; SARS; SARSM; SerRS; SerRSmt; SERS; SYS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208306 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCGTCCATGGCGCGGCGCTTGTGGCCTTTGCTGACTCGTCGGGGGTTCCGGCCCCGGGGAGGCT
  GCATCTCCAACGATAGTCCAAGGAGAAGTTTCACTACAGAGAAACGAAACCGGAACCTCCTGTACGAGTA
  TGCGCGCGAGGGCTACAGCGCACTCCCTCAGCTGGACATAGAGCGGTTCTGCGCATGCCCAGAAGAGGCC
  GCACACGCCCTGGAGCTCCGCAAGGGGGAGCTGCGCTCGGCGGACCTGCCCGCGATCATCTCGACATGGC
  AGGAGCTGAGGCAGCTGCAGGAGCAGATCCGGAGCCTGGAGGAAGAGAAGGCAGCTGTGACTGAGGCAGT
  GCGGGCCCTGCTGGCAAACCAGGACAGTGGTGAAGTGCAGCAGGACCCCAAGTACCAGGGTCTGCGGGCA
  CGTGGCCGGGAGATCCGGAAGGAGCTTGTTCACCTGTACCCCAGGGAGGCCCAGCTTGAGGAGCAGTTCT
  ACCTGCAGGCGCTGAAGCTGCCCAACCAGACCCACCCAGACGTGCCCGTCGGGGATGAGAGCCAGGCTCG
  AGTGCTCCACATGGTCGGAGACAAGCCAGTTTTCTCCTTCCAACCTCGGGGCCACCTGGAAATTGGCGAG
  AAACTCGACATCATCCGTCAGAAGCGCCTGTCCCACGTGTCTGGCCACCGGTCCTATTACCTGCGCGGGG
  CTGGAGCCCTCCTGCAGCACGGCCTGGTCAACTTCACATTCAACAAGCTTCTCCGCCGGGGCTTCACCCC
  CATGACGGTGCCAGACCTTCTCCGCGGAGCAGTGTTTGAAGGCTGTGGGATGACACCAAATGCCAACCCA
  TCCCAAATTTACAACATCGACCCTGCCCGCTTCAAAGATCTCAACCTGGCTGGAACAGCGGAGGTGGGGC
  TTGCAGGCTACTTCATGGACCACACCGTGGCCTTCAGGGACCTGCCAGTCAGGATGGTTTGCTCCAGCAC
  CTGCTACCGGGCAGAGACAAACACGGGACAGGAACCCCGGGGGCTGTATCGAGTACACCACTTCACCAAG
  GTGGAGATGTTTGGGGTGACAGGCCCTGGGCTGGAGCAGAGCTCACAGCTGCTGGAGGAGTTCCTGTCCC
  TTCAGATGGAGATCTTGACAGAGCTGGGCTTGCACTTCCGGGTCCTGGATATGCCCACCCAAGAACTGGG
  CCTCCCCGCCTACCGCAAGTTTGACATTGAGGCCTGGATGCCAGGCCGAGGCCGCTTTGGAGAGGTCACC
  AGTGCTTCCAACTGCACAGACTTCCAGAGCCGCCGCCTCCACATCATGTTCCAGACCGAGGCTGGGGAGC
  TGCAGTTTGCCCATACGGTGAACGCCACCGCCTGTGCTGTCCCCCGCCTTCTCATCGCGCTCCTGGAGAG
  TAACCAGCAGAAGGACGGCTCAGTGCTCGTGCCCCCTGCCCTCCAGTCCTACCTCGGCACTGATCGGATC
  ACAGCCCCTACCCACGTGCCTCTCCAGTACATCGGCCCCAACCAGCCCCGGAAGCCTGGGCTGCCTGGCC
  AGCCTGCTGTAAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208306 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAASMARRLWPLLTRRGFRPRGGCISNDSPRRSFTTEKRNRNLLYEYAREGYSALPQLDIERFCACPEEA
  AHALELRKGELRSADLPAIISTWQELRQLQEQIRSLEEEKAAVTEAVRALLANQDSGEVQQDPKYQGLRA
  RGREIRKELVHLYPREAQLEEQFYLQALKLPNQTHPDVPVGDESQARVLHMVGDKPVFSFQPRGHLEIGE
  KLDIIRQKRLSHVSGHRSYYLRGAGALLQHGLVNFTFNKLLRRGFTPMTVPDLLRGAVFEGCGMTPNANP
  SQIYNIDPARFKDLNLAGTAEVGLAGYFMDHTVAFRDLPVRMVCSSTCYRAETNTGQEPRGLYRVHHFTK
  VEMFGVTGPGLEQSSQLLEEFLSLQMEILTELGLHFRVLDMPTQELGLPAYRKFDIEAWMPGRGRFGEVT
  SASNCTDFQSRRLHIMFQTEAGELQFAHTVNATACAVPRLLIALLESNQQKDGSVLVPPALQSYLGTDRI
  TAPTHVPLQYIGPNQPRKPGLPGQPAVS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017827
• ORF Size: 1554 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017827.4
• RefSeq Size: 2077 bp
• RefSeq ORF: 1557 bp
• Locus ID: 54938
• UniProt ID: Q9NP81
• Cytogenetics: 19q13.2
• Domains: tRNA-synt_2b
• Protein Pathways: Aminoacyl-tRNA biosynthesis
• MW: 58.3 kDa
• Gene Summary: This gene encodes the mitochondrial seryl-tRNA synthethase precursor, a member of the class II tRNA synthetase family. The mature enzyme catalyzes the ligation of Serine to tRNA(Ser) and participates in the biosynthesis of selenocysteinyl-tRNA(sec) in mitochondria. The enzyme contains an N-terminal tRNA binding domain and a core catalytic domain. It functions in a homodimeric form, which is stabilized by tRNA binding. This gene is regulated by a bidirectional promoter that also controls the expression of mitochondrial ribosomal protein S12. Both genes are within the critical interval for the autosomal dominant deafness locus DFNA4 and might be linked to this disease. Multiple transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Mar 2009]