GGA1 (NM_001001560) Human Tagged ORF Clone

CAT#: RC208305

GGA1 (Myc-DDK-tagged)-Human golgi-associated, gamma adaptin ear containing, ARF binding protein 1 (GGA1), transcript variant 2

Product Images

Circular map for RC208305

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  Western blot validation of overexpression lysate (Cat# LY424381) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208305 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified GGA1 protein (Cat# TP308305). The protein was produced from HEK293T cells transfected with GGA1 cDNA clone (Cat# RC208305) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GGA1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208305 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCCGCGATGGAGCCGGAGACTCTGGAGGCGCGAATCAATAGAGCCACGAACCCCCTGAACAAGG
  AGCTCGACTGGGCCAGCATCAACGGCTTCTGCGAGCAGCTCAACGAGGACTTTGAGGGGCCTCCACTCGC
  CACCCGGCTGCTGGCCCACAAGATCCAGTCCCCACAGGAGTGGGAGGCGATCCAGGCCTTGACGGTGCTG
  GAAACATGCATGAAGAGCTGCGGCAAGCGGTTCCACGACGAAGTGGGCAAGTTCCGCTTTCTCAACGAGC
  TCATCAAGGTCGTGTCTCCCAAGTATCTGGGCTCTCGGACATCGGAGAAGGTGAAGAACAAGATCTTGGA
  GCTCCTCTACAGCTGGACAGTGGGCCTGCCCGAGGAGGTGAAAATCGCAGAGGCCTACCAGATGCTAAAG
  AAGCAGGGGATTGTAAAGTCCGACCCCAAGCTTCCAGATGACACTACCTTTCCCCTTCCTCCTCCACGGC
  CGAAGAATGTGATCTTTGAAGATGAGGAGAAATCCAAGATGCTGGCCCGCCTGCTGAAGAGCTCCCATCC
  CGAAGACCTCCGCGCAGCCAATAAGCTCATCAAAGAGATGGTGCAGGAGGACCAGAAGCGGATGGAGAAG
  ATCTCGAAGAGGGTGAATGCCATCGAGGAGGTGAACAACAATGTGAAACTGCTCACGGAGATGGTGATGA
  GCCACAGCCAGGGCGGCGCAGCAGCTGGCAGCAGCGAGGACCTCATGAAGGAACTGTACCAGCGCTGTGA
  GCGGATGCGGCCCACGCTCTTCCGACTGGCGAGTGACACAGAGGACAATGATGAGGCCTTAGGCCTCAGT
  GACCCCACACCCCCTTCAGGCCCAAGCCTGGATGGTACCGGATGGAACAGCTTCCAGTCGTCGGATGCCA
  CTGAGCCCCCAGCCCCTGCTCTGGCCCAGGCCCCCAGTATGGAAAGCCGACCCCCAGCGCAGACATCCCT
  GCCAGCAAGCAGCGGTCTGGACGACCTAGACCTCCTGGGGAAGACCCTCCTGCAGCAGTCGCTGCCCCCG
  GAATCCCAGCAAGTGCGGTGGGAGAAGCAGCAGCCAACCCCCCGGCTCACACTCCGGGACCTGCAGAATA
  AGAGCAGCAGCTGCAGCTCCCCCAGCTCCAGCGCCACCAGCCTTCTCCACACCGTGTCCCCAGAGCCCCC
  CAGGCCTCCGCAGCAGCCCGTACCAACCGAGCTCTCACTGGCCAGCATCACTGTGCCCCTGGAGTCCATC
  AAACCCAGCAACATCCTGCCCGTGACTGTGTATGACCAGCACGGCTTCCGCATCCTCTTCCATTTTGCCC
  GGGACCCACTGCCAGGGCGCTCCGACGTGCTGGTGGTGGTGGTTTCCATGCTGAGCACCGCCCCCCAGCC
  CATCCGCAACATCGTGTTCCAGTCAGCTGTCCCCAAGGTTATGAAGGTGAAGCTGCAGCCACCCTCGGGC
  ACGGAGCTGCCAGCTTTTAACCCCATCGTCCACCCCTCAGCAATCACCCAGGTCCTGCTGCTTGCCAACC
  CCCAGAAGGAGAAGGTTCGCCTCCGCTACAAGCTCACCTTCACCATGGGTGACCAGACCTACAACGAGAT
  GGGGGATGTGGACCAGTTCCCCCCACCTGAAACCTGGGGTAGCCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208305 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEPAMEPETLEARINRATNPLNKELDWASINGFCEQLNEDFEGPPLATRLLAHKIQSPQEWEAIQALTVL
  ETCMKSCGKRFHDEVGKFRFLNELIKVVSPKYLGSRTSEKVKNKILELLYSWTVGLPEEVKIAEAYQMLK
  KQGIVKSDPKLPDDTTFPLPPPRPKNVIFEDEEKSKMLARLLKSSHPEDLRAANKLIKEMVQEDQKRMEK
  ISKRVNAIEEVNNNVKLLTEMVMSHSQGGAAAGSSEDLMKELYQRCERMRPTLFRLASDTEDNDEALGLS
  DPTPPSGPSLDGTGWNSFQSSDATEPPAPALAQAPSMESRPPAQTSLPASSGLDDLDLLGKTLLQQSLPP
  ESQQVRWEKQQPTPRLTLRDLQNKSSSCSSPSSSATSLLHTVSPEPPRPPQQPVPTELSLASITVPLESI
  KPSNILPVTVYDQHGFRILFHFARDPLPGRSDVLVVVVSMLSTAPQPIRNIVFQSAVPKVMKVKLQPPSG
  TELPAFNPIVHPSAITQVLLLANPQKEKVRLRYKLTFTMGDQTYNEMGDVDQFPPPETWGSL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001001560
• ORF Size: 1656 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001001560.2, NP_001001560.1
• RefSeq Size: 2924 bp
• RefSeq ORF: 1659 bp
• Locus ID: 26088
• UniProt ID: Q9UJY5
• Cytogenetics: 22q13.1
• Protein Families: Druggable Genome
• Protein Pathways: Lysosome
• MW: 61.4 kDa
• Gene Summary: This gene encodes a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) protein family. Members of this family are ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network and the lysosome. These proteins share an amino-terminal VHS domain which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]