JPH4 (NM_032452) Human Tagged ORF Clone

CAT#: RC208221

JPH4 (Myc-DDK-tagged)-Human junctophilin 4 (JPH4), transcript variant 1

Product Images

Circular map for RC208221

• 

  Western blot validation of overexpression lysate (Cat# LY432005) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC228978 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified JPH4 protein (Cat# TP308221). The protein was produced from HEK293T cells transfected with JPH4 cDNA clone (Cat# RC208221) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: JPH4
• Synonyms: JP4; JPHL1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208221 representing NM_032452
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCCCGGGGGCAAGTTCGACTTTGACGACGGGGGCTGCTACGTGGGGGGCTGGGAGGCGGGGCGGG
  CACATGGCTACGGCGTGTGCACGGGCCCCGGCGCCCAGGGCGAGTACAGCGGCTGCTGGGCACACGGCTT
  CGAGTCACTGGGCGTCTTCACGGGGCCCGGCGGACACAGCTACCAGGGCCACTGGCAGCAGGGCAAGCGC
  GAAGGGCTGGGCGTGGAGCGCAAGAGCCGCTGGACGTACCGCGGCGAGTGGCTGGGCGGGCTGAAGGGGC
  GCAGCGGCGTGTGGGAAAGCGTGTCCGGCCTGCGCTACGCCGGGCTCTGGAAGGACGGTTTCCAGGACGG
  CTACGGCACTGAGACCTACTCCGACGGAGGCACCTACCAGGGCCAGTGGCAGGCCGGGAAGCGCCACGGC
  TACGGGGTACGCCAGAGTGTGCCCTACCATCAGGCGGCGCTGCTGCGCTCGCCCCGCCGCACCTCCCTGG
  ATTCCGGCCACAGCGACCCCCCGACGCCACCCCCGCCCCTGCCCTTGCCGGGCGACGAGGGAGGCAGCCC
  CGCCTCGGGCTCCCGGGGCGGCTTCGTGCTGGCCGGGCCCGGGGACGCCGACGGCGCGTCGTCCCGAAAG
  CGCACTCCAGCGGCCGGCGGATTCTTTCGCCGTTCGCTGCTGCTCAGCGGGCTCCGAGCGGGCGGACGTC
  GCAGCTCCCTGGGCAGCAAGCGAGGCTCCCTGCGCAGCGAGGTGAGCAGCGAGGTGGGCAGCACCGGACC
  GCCCGGCTCGGAGGCCAGCGGGCCCCCGGCCGCAGCGCCGCCCGCCCTCATCGAGGGCTCGGCCACAGAG
  GTGTACGCGGGCGAGTGGCGCGCAGATCGGCGCAGCGGCTTCGGCGTCAGCCAGCGCTCCAACGGGCTGC
  GCTACGAGGGCGAGTGGCTGGGCAACCAGCGGCACGGCTACGGGCGCACCACCCGCCCCGACGGCTCCCG
  CGAGGAGGGCAAGTACAAGCGCAACCGGCTGGTGCACGGCGGGCGCGTCCGCAGTCTCCTGCCTCTGGCC
  CTTCGGCGGGGCAAGGTTAAGGAGAAGGTGGACAGGGCTGTCGAGGGCGCCCGTCGAGCCGTGAGTGCTG
  CCCGTCAGCGCCAGGAGATCGCCGCTGCCAGGGCAGCAGACGCCCTCCTAAAGGCAGTGGCAGCCAGCAG
  TGTCGCTGAGAAGGCCGTGGAGGCAGCTCGAATGGCCAAACTGATAGCCCAGGACCTGCAGCCCATGCTA
  GAGGCCCCAGGCCGCAGACCCAGGCAGGACTCAGAAGGTTCCGACACGGAGCCCCTGGATGAGGACAGCC
  CTGGGGTATATGAGAACGGACTGACCCCCTCAGAGGGATCCCCTGAACTGCCCAGCAGTCCTGCCTCCTC
  CCGCCAACCCTGGCGACCCCCTGCCTGCCGGAGCCCACTGCCTCCTGGAGGGGACCAGGGTCCCTTCTCC
  AGCCCCAAAGCTTGGCCTGAGGAGTGGGGGGGGGCAGGCGCACAGGCAGAGGAACTAGCTGGCTATGAGG
  CTGAGGATGAGGCTGGGATGCAAGGGCCAGGGCCCAGAGACGGTTCCCCACTCCTCGGAGGCTGCAGCGA
  CAGTTCAGGAAGTCTTCGAGAGGAGGAGGGGGAGGATGAAGAGCCCCTGCCCCCGCTGAGGGCCCCAGCA
  GGCACGGAGCCTGAGCCCATCGCCATGCTGGTCCTGAGGGGCTCGTCCTCGAGGGGTCATGATGCTGGGT
  GCCTGACAGAAGAGCTCGGGGAGCCCGCTGCAACCGAGAGGCCTGCCCAGCCGGGAGCTGCCAACCCCCT
  GGTGGTGGGAGCCGTGGCCCTCCTGGACCTCAGCCTGGCATTCCTGTTCTCCCAGCTCCTCACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208221 representing NM_032452
  Red=Cloning site Green=Tags(s)
  
  MSPGGKFDFDDGGCYVGGWEAGRAHGYGVCTGPGAQGEYSGCWAHGFESLGVFTGPGGHSYQGHWQQGKR
  EGLGVERKSRWTYRGEWLGGLKGRSGVWESVSGLRYAGLWKDGFQDGYGTETYSDGGTYQGQWQAGKRHG
  YGVRQSVPYHQAALLRSPRRTSLDSGHSDPPTPPPPLPLPGDEGGSPASGSRGGFVLAGPGDADGASSRK
  RTPAAGGFFRRSLLLSGLRAGGRRSSLGSKRGSLRSEVSSEVGSTGPPGSEASGPPAAAPPALIEGSATE
  VYAGEWRADRRSGFGVSQRSNGLRYEGEWLGNQRHGYGRTTRPDGSREEGKYKRNRLVHGGRVRSLLPLA
  LRRGKVKEKVDRAVEGARRAVSAARQRQEIAAARAADALLKAVAASSVAEKAVEAARMAKLIAQDLQPML
  EAPGRRPRQDSEGSDTEPLDEDSPGVYENGLTPSEGSPELPSSPASSRQPWRPPACRSPLPPGGDQGPFS
  SPKAWPEEWGGAGAQAEELAGYEAEDEAGMQGPGPRDGSPLLGGCSDSSGSLREEEGEDEEPLPPLRAPA
  GTEPEPIAMLVLRGSSSRGHDAGCLTEELGEPAATERPAQPGAANPLVVGAVALLDLSLAFLFSQLLT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032452
• ORF Size: 1884 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032452.1, NP_115828.1
• RefSeq Size: 4396 bp
• RefSeq ORF: 1887 bp
• Locus ID: 84502
• UniProt ID: Q96JJ6
• Cytogenetics: 14q11.2
• MW: 65.7 kDa
• Gene Summary: This gene encodes a member of the junctophilin family of transmembrane proteins that are involved in the formation of the junctional membrane complexes between the plasma membrane and the endoplasmic/sarcoplasmic reticulum in excitable cells. The encoded protein contains a conserved N-terminal repeat region called the membrane occupation and recognition nexus sequence that is found in other members of the junctophilin family. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2009]