BMS1L (BMS1) (NM_014753) Human Tagged ORF Clone

CAT#: RC208119

BMS1 (Myc-DDK-tagged)-Human BMS1 homolog, ribosome assembly protein (yeast) (BMS1)

Product Images

Circular map for RC208119

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BMS1L
• Synonyms: ACC; BMS1L
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208119 representing NM_014753
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGCTAAGGACCAGAAGAAACACAGAAAGAAAAACAGTGGACCCAAAGCTGCAAAGAAAAAGAAGC
  GGCTTCTGCAGGATCTCCAGCTAGGAGACGAAGAAGATGCCCGGAAGAGAAATCCCAAAGCTTTTGCAGT
  TCAGTCTGCTGTGCGGATGGCTCGATCCTTTCACAGGACTCAGGATTTGAAGACAAAAAAGCATCATATT
  CCAGTGGTTGATCGAACTCCACTAGAGCCCCCACCAATAGTGGTAGTGGTGATGGGACCTCCAAAAGTTG
  GAAAGAGCACTTTGATACAATGCCTCATTCGGAACTTTACCCGGCAGAAGTTGACTGAGATCAGAGGCCC
  TGTGACGATTGTGTCAGGTAAAAAGCGCAGACTCACCATTATTGAATGTGGGTGTGACATTAACATGATG
  ATTGATCTGGCTAAAGTAGCAGATCTGGTACTGATGCTTATAGATGCCAGCTTTGGGTTTGAAATGGAAA
  CGTTTGAGTTTCTAAACATCTGTCAAGTACATGGCTTTCCTAAAATTATGGGAGTTCTCACCCACCTCGA
  CTCCTTCAAGCATAATAAGCAACTGAAGAAGACAAAGAAGCGATTAAAACACAGGTTCTGGACGGAAGTT
  TACCCGGGTGCCAAGCTGTTCTACCTTTCTGGAATGGTGCATGGAGAATATCAAAACCAAGAAATCCACA
  ATCTGGGCCGTTTTATTACAGTTATGAAGTTTAGGCCTCTCACATGGCAAACTTCTCACCCTTATATCCT
  GGCAGACAGGATGGAAGATTTGACAAACCCAGAGGATATCCGAACAAACATCAAATGTGACCGGAAGGTG
  TCACTTTATGGTTATTTAAGAGGAGCACACTTGAAAAATAAAAGCCAAATTCACATGCCAGGGGTAGGAG
  ATTTTGCCGTGAGTGACATCAGTTTCCTCCCAGACCCTTGCGCTCTTCCTGAACAACAAAAGAAGCGCTG
  TTTAAATGAGAAGGAGAAGCTGGTTTATGCGCCTCTTTCTGGAGTTGGGGGTGTGCTGTATGACAAAGAC
  GCTGTCTATGTTGACCTTGGTGGCAGCCACGTTTTTCAGGATGAAGTGGGGCCCACCCATGAGCTGGTCC
  AGAGTCTCATCTCTACCCACTCCACCATTGATGCCAAGATGGCTTCAAGTCGAGTGACGCTGTTTTCTGA
  TTCCAAGCCACTTGGGTCAGAGGATATAGATAATCAAGGGCTAATGATGCCAAAGGAGGAAAAACAAATG
  GACTTGAACACTGGTCGAATGCGTCGGAAAGCCATTTTCGGAGATGAAGATGAATCTGGAGATAGTGATG
  ATGAAGAAGATGATGAAATGTCTGAAGATGACGGGTTGGAAAACGGCTCTAGTGATGAGGAAGCAGAAGA
  GGAGGAAAATGCTGAGATGACTGATCAGTATATGGCTGTTAAGGGCATCAAACGACGGAAACTTGAGTTG
  GAAGAAGACAGTGAAATGGATTTGCCAGCATTTGCTGACAGTGACGATGACCTTGAGAGGAGCTCAGCGG
  AAGAAGGGGAAGCGGAGGAAGCTGATGAAAGCAGTGAAGAAGAGGACTGCACTGCAGGAGAGAAGGGCAT
  TTCAGGATCAAAGGCTGCTGGAGAAGGTAGTAAAGCAGGGCTGTCACCAGCTAATTGCCAGAGTGACCGT
  GTGAATCTGGAGAAGTCTTTGCTGATGAAGAAAGCAGCTCTCCCCACTTTCGATTCTGGGCATTGCACAG
  CTGAAGAGGTGTTTGCATCTGAAGATGAATCTGAAGAAAGCTCCTCACTCAGTGCAGAGGAAGAAGACTC
  AGAAAATGAAGAGGCTATTAGAAAAAAGCTTTCAAAGCCTTCTCAAGTGAGCAGTGGTCAGAAACTGGGG
  CCACAGAACTTCATTGATGAGACCAGTGATATAGAAAATTTACTCAAAGAGGAAGAAGATTACAAGGAAG
  AAAATAATGATTCCAAAGAAACGTCAGGTGCCCTCAAGTGGAAGGAAGACCTTTCCAGAAAGGCAGCTGA
  GGCCTTTCTGAGGCAGCAGCAAGCAGCTCCAAACCTCCGAAAGCTTATTTATGGGACAGTGACAGAAGAT
  AATGAAGAAGAAGATGATGATACTCTAGAAGAGCTTGGAGGGTTGTTTCGTGTCAACCAGCCTGACAGAG
  AGTGTAAGCACAAGGCTGACTCTTTGGACTGCTCCAGATTTCTTGTGGAGGCCCCCCATGACTGGGATTT
  AGAGGAGGTTATGAACAGTATCAGAGATTGCTTCGTGACTGGAAAGTGGGAAGATGATAAAGATGCAGCC
  AAGGTCTTAGCAGAAGATGAGGAGCTCTACGGTGACTTTGAAGACTTGGAAACAGGGGACGTGCACAAGG
  GAAAATCAGGCCCCAATACTCAGAATGAAGATATAGAGAAAGAAGTTAAGGAAGAAATTGACCCCGACGA
  AGAAGAAAGTGCCAAGAAAAAGCATTTGGATAAGAAGAGAAAATTGAAGGAGATGTTTGATGCAGAATAT
  GATGAAGGAGAAAGCACATATTTTGATGATCTTAAAGGAGAAATGCAGAAACAAGCACAGCTGAATCGCG
  CAGAATTTGAAGATCAAGATGATGAAGCCAGAGTTCAGTATGAGGGTTTTCGACCTGGGATGTACGTCCG
  CATTGAGATTGAAAATGTTCCCTGTGAATTTGTGCAGAACTTTGACCCCCATTACCCCATTATCCTGGGT
  GGCTTGGGCAACAGTGAGGGAAATGTTGGCTACGTGCAGATGCGTCTGAAGAAACATCGCTGGTATAAGA
  AAATCCTCAAGTCCCGAGATCCAATCATATTTTCTGTAGGGTGGAGGAGGTTTCAGACCATCCCACTGTA
  TTATATCGAAGACCACAATGGAAGACAAAGGCTTCTAAAGTATACCCCACAGCACATGCATTGCGGAGCA
  GCCTTTTGGGGCCCTATCACTCCACAGGGAACTGGTTTCTTGGCAATACAGTCTGTCAGTGGCATAATGC
  CTGATTTTCGGATAGCTGCTACAGGAGTTGTCCTTGATCTGGATAAATCCATAAAAATTGTGAAGAAATT
  AAAGCTAACTGGTTTTCCATATAAAATTTTCAAGAACACTTCATTTATTAAGGGAATGTTTAATTCTGCC
  TTGGAAGTGGCCAAATTTGAAGGTGCTGTGATTCGAACAGTCAGTGGGATAAGGGGGCAGATCAAGAAAG
  CACTCCGAGCTCCAGAAGGAGCTTTCAGGGCCAGCTTTGAGGATAAGCTGCTGATGAGCGATATTGTCTT
  CATGCGAACTTGGTATCCTGTTTCCATCCCAGCGTTCTATAACCCAGTAACATCTTTGTTGAAACCAGTG
  GGTGAGAAAGACACCTGGTCAGGAATGCGGACCACGGGCCAACTCAGGCTCGCCCATGGCGTCAGACTAA
  AGGCGAACAAGGACTCTCTGTATAAGCCAATCCTGAGGCAAAAGAAACATTTTAATTCACTGCACATTCC
  AAAAGCCTTGCAGAAGGCCCTGCCATTTAAGAACAAGCCCAAGACCCAAGCAAAGGCAGGCAAGGTGCCA
  AAGGACAGGCGGAGACCGGCCGTCATACGCGAGCCTCATGAAAGAAAGATCCTTGCACTGCTGGATGCTC
  TGAGTACGGTGCATAGTCAGAAGATGAAGAAGGCCAAGGAGCAGCGGCACCTGCACAATAAAGAGCACTT
  CAGAGCCAAGCAGAAGGAGGAGGAGGAGAAGCTGAAGCGGCAGAAGGACCTCAGGAAGAAGCTCTTCAGA
  ATTCAGGGGCAGAAGGAAAGAAGAAACCAGAAGTCCAGTTTGAAGGGGGCTGAGGGCCAATTGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208119 representing NM_014753
  Red=Cloning site Green=Tags(s)
  
  MEAKDQKKHRKKNSGPKAAKKKKRLLQDLQLGDEEDARKRNPKAFAVQSAVRMARSFHRTQDLKTKKHHI
  PVVDRTPLEPPPIVVVVMGPPKVGKSTLIQCLIRNFTRQKLTEIRGPVTIVSGKKRRLTIIECGCDINMM
  IDLAKVADLVLMLIDASFGFEMETFEFLNICQVHGFPKIMGVLTHLDSFKHNKQLKKTKKRLKHRFWTEV
  YPGAKLFYLSGMVHGEYQNQEIHNLGRFITVMKFRPLTWQTSHPYILADRMEDLTNPEDIRTNIKCDRKV
  SLYGYLRGAHLKNKSQIHMPGVGDFAVSDISFLPDPCALPEQQKKRCLNEKEKLVYAPLSGVGGVLYDKD
  AVYVDLGGSHVFQDEVGPTHELVQSLISTHSTIDAKMASSRVTLFSDSKPLGSEDIDNQGLMMPKEEKQM
  DLNTGRMRRKAIFGDEDESGDSDDEEDDEMSEDDGLENGSSDEEAEEEENAEMTDQYMAVKGIKRRKLEL
  EEDSEMDLPAFADSDDDLERSSAEEGEAEEADESSEEEDCTAGEKGISGSKAAGEGSKAGLSPANCQSDR
  VNLEKSLLMKKAALPTFDSGHCTAEEVFASEDESEESSSLSAEEEDSENEEAIRKKLSKPSQVSSGQKLG
  PQNFIDETSDIENLLKEEEDYKEENNDSKETSGALKWKEDLSRKAAEAFLRQQQAAPNLRKLIYGTVTED
  NEEEDDDTLEELGGLFRVNQPDRECKHKADSLDCSRFLVEAPHDWDLEEVMNSIRDCFVTGKWEDDKDAA
  KVLAEDEELYGDFEDLETGDVHKGKSGPNTQNEDIEKEVKEEIDPDEEESAKKKHLDKKRKLKEMFDAEY
  DEGESTYFDDLKGEMQKQAQLNRAEFEDQDDEARVQYEGFRPGMYVRIEIENVPCEFVQNFDPHYPIILG
  GLGNSEGNVGYVQMRLKKHRWYKKILKSRDPIIFSVGWRRFQTIPLYYIEDHNGRQRLLKYTPQHMHCGA
  AFWGPITPQGTGFLAIQSVSGIMPDFRIAATGVVLDLDKSIKIVKKLKLTGFPYKIFKNTSFIKGMFNSA
  LEVAKFEGAVIRTVSGIRGQIKKALRAPEGAFRASFEDKLLMSDIVFMRTWYPVSIPAFYNPVTSLLKPV
  GEKDTWSGMRTTGQLRLAHGVRLKANKDSLYKPILRQKKHFNSLHIPKALQKALPFKNKPKTQAKAGKVP
  KDRRRPAVIREPHERKILALLDALSTVHSQKMKKAKEQRHLHNKEHFRAKQKEEEEKLKRQKDLRKKLFR
  IQGQKERRNQKSSLKGAEGQLQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014753
• ORF Size: 3846 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014753.4
• RefSeq Size: 8048 bp
• RefSeq ORF: 3849 bp
• Locus ID: 9790
• UniProt ID: Q14692
• Cytogenetics: 10q11.21
• Domains: DUF663
• MW: 145.81 kDa
• Gene Summary: This gene likely encodes a ribosome assembly protein. A similar protein in yeast functions in 35S-rRNA processing, which includes a series of cleavage steps critical for formation of 40S ribosomes. Related pseudogenes exist on chromosomes 2, 9, 10, 15, 16, and 22.[provided by RefSeq, Mar 2009]