FECH (NM_000140) Human Tagged ORF Clone

CAT#: RC208058

FECH (Myc-DDK-tagged)-Human ferrochelatase (FECH), nuclear gene encoding mitochondrial protein, transcript variant 2

Product Images

Circular map for RC208058

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  Western blot validation of overexpression lysate (Cat# LY400049) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208058 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified FECH protein (Cat# TP308058). The protein was produced from HEK293T cells transfected with FECH cDNA clone (Cat# RC208058) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FECH
• Synonyms: EPP; EPP1; FCE
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208058 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGTTCACTCGGCGCAAACATGGCTGCGGCCCTGCGCGCCGCGGGCGTCCTGCTCCGCGATCCGCTGG
  CATCCAGCAGCTGGAGGGTCTGTCAGCCATGGAGGTGGAAGTCAGGTGCAGCTGCAGCGGCCGTCACCAC
  AGAAACAGCCCAGCATGCCCAGGGTGCAAAACCTCAAGTTCAACCGCAGAAGAGGAAGCCGAAAACTGGA
  ATATTAATGCTAAACATGGGAGGCCCTGAAACTCTTGGAGATGTTCACGACTTCCTTCTGAGACTCTTCT
  TGGACCGAGACCTCATGACACTTCCTATTCAGAATAAGCTGGCACCATTCATCGCCAAACGCCGAACCCC
  CAAGATTCAAGAGCAGTACCGCAGGATTGGAGGCGGATCCCCCATCAAGATATGGACTTCCAAGCAGGGA
  GAGGGCATGGTGAAGCTGCTGGATGAATTGTCCCCCAACACAGCCCCTCACAAATACTATATTGGATTTC
  GGTACGTCCATCCTTTAACAGAAGAAGCAATTGAAGAGATGGAGAGAGATGGCCTAGAAAGGGCTATTGC
  TTTCACACAGTATCCACAGTACAGCTGCTCCACCACAGGCAGCAGCTTAAATGCCATTTACAGATACTAT
  AATCAAGTGGGACGGAAGCCCACGATGAAGTGGAGCACTATTGACAGGTGGCCCACACATCACCTCCTCA
  TCCAGTGCTTTGCAGATCATATTCTAAAGGAACTGGACCATTTTCCACTTGAGAAGAGAAGCGAGGTGGT
  CATTCTGTTTTCTGCTCACTCACTGCCCATGTCTGTGGTCAACAGAGGCGACCCATATCCTCAGGAGGTA
  AGCGCCACTGTCCAAAAAGTCATGGAAAGGCTGGAGTACTGCAACCCCTACCGACTGGTGTGGCAATCCA
  AGGTTGGTCCAATGCCCTGGTTGGGTCCTCAAACAGACGAATCTATCAAAGGGCTTTGTGAGAGGGGGAG
  GAAGAATATCCTCTTGGTTCCGATAGCATTTACCAGTGACCATATTGAAACGCTGTATGAGCTGGACATC
  GAGTACTCTCAAGTTTTAGCCAAGGAGTGTGGAGTTGAAAACATCAGAAGAGCTGAGTCTCTTAATGGAA
  ATCCATTGTTCTCTAAGGCCCTGGCCGACTTGGTGCATTCACACATCCAGTCAAACGAGCTGTGTTCCAA
  GCAGCTGACCCTGAGCTGTCCGCTCTGTGTCAATCCTGTCTGCAGGGAGACTAAATCCTTCTTCACCAGC
  CAGCAGCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208058 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MRSLGANMAAALRAAGVLLRDPLASSSWRVCQPWRWKSGAAAAAVTTETAQHAQGAKPQVQPQKRKPKTG
  ILMLNMGGPETLGDVHDFLLRLFLDRDLMTLPIQNKLAPFIAKRRTPKIQEQYRRIGGGSPIKIWTSKQG
  EGMVKLLDELSPNTAPHKYYIGFRYVHPLTEEAIEEMERDGLERAIAFTQYPQYSCSTTGSSLNAIYRYY
  NQVGRKPTMKWSTIDRWPTHHLLIQCFADHILKELDHFPLEKRSEVVILFSAHSLPMSVVNRGDPYPQEV
  SATVQKVMERLEYCNPYRLVWQSKVGPMPWLGPQTDESIKGLCERGRKNILLVPIAFTSDHIETLYELDI
  EYSQVLAKECGVENIRRAESLNGNPLFSKALADLVHSHIQSNELCSKQLTLSCPLCVNPVCRETKSFFTS
  QQL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000140
• ORF Size: 1269 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000140.5
• RefSeq Size: 7277 bp
• RefSeq ORF: 1272 bp
• Locus ID: 2235
• UniProt ID: P22830
• Cytogenetics: 18q21.31
• Domains: Ferrochelatase
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Porphyrin and chlorophyll metabolism
• MW: 47.9 kDa
• Gene Summary: The protein encoded by this gene is localized to the mitochondrion, where it catalyzes the insertion of the ferrous form of iron into protoporphyrin IX in the heme synthesis pathway. Mutations in this gene are associated with erythropoietic protoporphyria. Two transcript variants encoding different isoforms have been found for this gene. A pseudogene of this gene is found on chromosome 3.[provided by RefSeq, May 2010]