TBC1D1 (NM_015173) Human Tagged ORF Clone

CAT#: RC207980

TBC1D1 (Myc-DDK-tagged)-Human TBC1 (tre-2/USP6, BUB2, cdc16) domain family, member 1 (TBC1D1)

Product Images

Circular map for RC207980

• 

  Western blot validation of overexpression lysate (Cat# LY414764) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207980 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified TBC1D1 protein (Cat# TP307980). The protein was produced from HEK293T cells transfected with TBC1D1 cDNA clone (Cat# RC207980) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TBC1D1
• Synonyms: TBC; TBC1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207980 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACCAATAACATTCACAGCAAGGAAACATCTGCTTTCTAACGAGGTCTCGGTGGATTTTGGCCTGC
  AGCTGGTGGGCTCCCTGCCTGTGCATTCCCTGACCACCATGCCCATGCTGCCCTGGGTTGTGGCTGAGGT
  GCGAAGACTCAGCAGGCAGTCCACCAGAAAGGAACCTGTAACCAAGCAAGTCCGGCTTTGCGTTTCACCC
  TCTGGACTGAGATGTGAACCTGAGCCAGGGAGAAGTCAACAGTGGGATCCCCTGATCTATTCCAGCATCT
  TTGAGTGCAAGCCTCAGCGTGTTCACAAACTGATTCACAACAGTCATGACCCAAGTTACTTTGCTTGTCT
  GATTAAGGAAGACGCTGTCCACCGGCAGAGTATCTGCTATGTGTTCAAAGCCGATGATCAAACAAAAGTG
  CCTGAGATCATCAGCTCCATCCGTCAGGCGGGGAAGATCGCCCGGCAGGAGGAGCTGCACTGCCCGTCCG
  AGTTCGACGACACGTTTTCCAAGAAGTTCGAGGTGCTCTTCTGCGGCCGCGTGACGGTGGCGCACAAGAA
  GGCTCCGCCGGCCCTGATCGACGAGTGCATCGAGAAGTTCAATCACGTCAGCGGCAGCCGGGGGTCCGAG
  AGCCCCCGCCCCAACCCGCCCCATGCCGCGCCCACAGGGAGCCAGGAGCCTGTGCGCAGGCCCATGCGCA
  AGTCCTTCTCCCAGCCCGGCCTGCGCTCGCTGGCCTTTAGGAAGGAGCTGCAGGATGGGGGCCTCCGAAG
  CAGCGGCTTCTTCAGCTCCTTCGAGGAGAGCGACATTGAGAACCACCTCATTAGCGGACACAATATTGTG
  CAGCCCACAGATATCGAGGAAAATCGAACTATGCTCTTCACGATTGGCCAGTCTGAAGTTTACCTCATCA
  GTCCTGACACCAAAAAAATAGCATTGGAGAAAAATTTTAAGGAGATATCCTTTTGCTCTCAGGGCATCAG
  ACACGTGGACCACTTTGGGTTTATCTGTCGGGAGTCTTCCGGAGGTGGCGGCTTTCATTTTGTCTGTTAC
  GTGTTTCAGTGCACAAATGAGGCTCTGGTTGATGAAATTATGATGACCCTGAAACAGGCCTTCACGGTGG
  CCGCAGTGCAGCAGACAGCTAAGGCGCCAGCCCAGCTGTGTGAGGGCTGCCCCCTGCAAAGCCTGCACAA
  GCTCTGTGAGAGGATAGAGGGAATGAATTCTTCCAAAACAAAACTAGAACTGCAAAAGCACCTGACGACA
  TTAACAAATCAGGAGCAGGCGACTATTTTTGAAGAGGTTCAGAAATTGAGACCGAGAAATGAGCAGCGAG
  AGAATGAATTGATTATTTCTTTTCTGAGATGTTTATATGAAGAGAAACAGAAAGAACACATCCATATTGG
  GGAGATGAAGCAGACATCGCAGATGGCAGCAGAGAATATTGGAAGTGAATTACCACCCAGTGCCACTCGA
  TTTAGGCTAGATATGCTGAAAAACAAAGCAAAGAGATCTTTAACAGAGTCTTTAGAAAGTATTTTGTCCC
  GGGGTAATAAAGCCAGAGGCCTGCAGGAACACTCCATCAGTGTGGATCTGGATAGCTCCCTGTCTAGTAC
  ATTAAGTAACACCAGCAAAGAGCCATCTGTGTGTGAAAAGGAGGCCTTGCCCATCTCTGAGAGCTCCTTT
  AAGCTCCTCGGCTCCTCGGAGGACCTGTCCAGTGACTCGGAGAGTCATCTCCCAGAAGAGCCAGCTCCGC
  TGTCGCCCCAGCAGGCCTTCAGGAGGCGAGCAAACACCCTGAGTCACTTCCCCATCGAATGCCAGGAACC
  TCCACAACCTGCCCGGGGGTCCCCGGGGGTTTCGCAAAGGAAACTTATGAGGTATCACTCAGTGAGCACA
  GAGACGCCTCATGAACGAAAGGACTTTGAATCCAAAGCAAACCATCTTGGTGATTCTGGTGGGACTCCTG
  TGAAGACCCGGAGGCATTCCTGGAGGCAGCAGATATTCCTCCGAGTAGCCACCCCGCAGAAGGCGTGCGA
  TTCTTCCAGCAGATATGAAGATTATTCAGAGCTGGGAGAGCTTCCCCCACGATCTCCTTTAGAACCAGTT
  TGTGAAGATGGGCCCTTTGGCCCCCCACCAGAGGAAAAGAAAAGGACATCTCGTGAGCTCCGAGAGCTGT
  GGCAAAAGGCTATTCTTAAACAGATACTGCTGCTTAGAATGGAGAAGGAAAATCAGAAGCTCCAAGCCTC
  TGAAAATGATTTGCTGAACAAGCGCCTGAAGCTCGATTATGAAGAAATTACTCCCTGTCTTAAAGAAGTA
  ACTACAGTGTGGGAAAAGATGCTTAGCACTCCAGGAAGATCAAAAATTAAGTTTGACATGGAAAAAATGC
  ACTCGGCTGTTGGGCAAGGTGTGCCACGTCATCACCGAGGTGAAATCTGGAAATTTCTAGCTGAGCAATT
  CCACCTTAAACACCAGTTTCCCAGCAAACAGCAGCCAAAGGATGTGCCATACAAAGAACTCTTAAAGCAG
  CTGACTTCCCAGCAGCATGCGATTCTTATTGACCTTGGGCGAACCTTTCCTACACACCCATACTTCTCTG
  CCCAGCTTGGAGCAGGACAGCTATCGCTTTACAACATTTTGAAGGCCTACTCACTTCTAGACCAGGAAGT
  GGGATATTGCCAAGGTCTCAGCTTTGTAGCAGGCATTTTGCTTCTTCATATGAGTGAGGAAGAGGCGTTT
  AAAATGCTCAAGTTTCTGATGTTTGACATGGGGCTGCGGAAACAGTATCGGCCAGACATGATTATTTTAC
  AGATCCAGATGTACCAGCTCTCGAGGTTGCTTCATGATTACCACAGAGACCTCTACAATCACCTGGAGGA
  GCACGAGATCGGCCCCAGCCTCTACGCTGCCCCCTGGTTCCTCACCATGTTTGCCTCACAGTTCCCGCTG
  GGATTCGTAGCCAGAGTCTTTGATATGATTTTTCTTCAGGGAACAGAGGTCATATTTAAAGTGGCTTTAA
  GTCTGTTGGGAAGCCATAAGCCCTTGATTCTGCAGCATGAAAACCTAGAAACCATAGTTGACTTTATAAA
  AAGCACGCTACCCAACCTTGGCTTGGTACAGATGGAAAAGACCATCAATCAGGTATTTGAAATGGACATC
  GCTAAACAGTTACAAGCTTATGAAGTTGAGTACCACGTCCTTCAAGAAGAACTTATCGATTCCTCTCCTC
  TCAGTGACAACCAAAGAATGGATAAATTAGAGAAAACCAACAGCAGCTTACGCAAACAGAACCTTGACCT
  CCTTGAACAGTTGCAGGTGGCAAATGGTAGGATCCAAAGCCTTGAGGCCACCATTGAGAAGCTCCTGAGC
  AGTGAGAGCAAGCTGAAGCAGGCCATGCTTACCTTAGAACTGGAGCGGTCGGCCCTGCTGCAGACGGTGG
  AGGAGCTGCGGCGGCGGAGCGCAGAGCCCAGCGACCGGGAGCCTGAGTGCACGCAGCCCGAGCCCACGGG
  CGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207980 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEPITFTARKHLLSNEVSVDFGLQLVGSLPVHSLTTMPMLPWVVAEVRRLSRQSTRKEPVTKQVRLCVSP
  SGLRCEPEPGRSQQWDPLIYSSIFECKPQRVHKLIHNSHDPSYFACLIKEDAVHRQSICYVFKADDQTKV
  PEIISSIRQAGKIARQEELHCPSEFDDTFSKKFEVLFCGRVTVAHKKAPPALIDECIEKFNHVSGSRGSE
  SPRPNPPHAAPTGSQEPVRRPMRKSFSQPGLRSLAFRKELQDGGLRSSGFFSSFEESDIENHLISGHNIV
  QPTDIEENRTMLFTIGQSEVYLISPDTKKIALEKNFKEISFCSQGIRHVDHFGFICRESSGGGGFHFVCY
  VFQCTNEALVDEIMMTLKQAFTVAAVQQTAKAPAQLCEGCPLQSLHKLCERIEGMNSSKTKLELQKHLTT
  LTNQEQATIFEEVQKLRPRNEQRENELIISFLRCLYEEKQKEHIHIGEMKQTSQMAAENIGSELPPSATR
  FRLDMLKNKAKRSLTESLESILSRGNKARGLQEHSISVDLDSSLSSTLSNTSKEPSVCEKEALPISESSF
  KLLGSSEDLSSDSESHLPEEPAPLSPQQAFRRRANTLSHFPIECQEPPQPARGSPGVSQRKLMRYHSVST
  ETPHERKDFESKANHLGDSGGTPVKTRRHSWRQQIFLRVATPQKACDSSSRYEDYSELGELPPRSPLEPV
  CEDGPFGPPPEEKKRTSRELRELWQKAILKQILLLRMEKENQKLQASENDLLNKRLKLDYEEITPCLKEV
  TTVWEKMLSTPGRSKIKFDMEKMHSAVGQGVPRHHRGEIWKFLAEQFHLKHQFPSKQQPKDVPYKELLKQ
  LTSQQHAILIDLGRTFPTHPYFSAQLGAGQLSLYNILKAYSLLDQEVGYCQGLSFVAGILLLHMSEEEAF
  KMLKFLMFDMGLRKQYRPDMIILQIQMYQLSRLLHDYHRDLYNHLEEHEIGPSLYAAPWFLTMFASQFPL
  GFVARVFDMIFLQGTEVIFKVALSLLGSHKPLILQHENLETIVDFIKSTLPNLGLVQMEKTINQVFEMDI
  AKQLQAYEVEYHVLQEELIDSSPLSDNQRMDKLEKTNSSLRKQNLDLLEQLQVANGRIQSLEATIEKLLS
  SESKLKQAMLTLELERSALLQTVEELRRRSAEPSDREPECTQPEPTGD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015173
• ORF Size: 3504 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015173.4
• RefSeq Size: 5705 bp
• RefSeq ORF: 3507 bp
• Locus ID: 23216
• UniProt ID: Q86TI0
• Cytogenetics: 4p14
• Domains: TBC
• Protein Families: Druggable Genome
• MW: 133.1 kDa
• Gene Summary: TBC1D1 is the founding member of a family of proteins sharing a 180- to 200-amino acid TBC domain presumed to have a role in regulating cell growth and differentiation. These proteins share significant homology with TRE2 (USP6; MIM 604334), yeast Bub2, and CDC16 (MIM 603461) (White et al., 2000 [PubMed 10965142]).[supplied by OMIM, Mar 2008]