TBC1D1 (NM_015173) Human Tagged ORF Clone

CAT#: RC207980

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TBC1D1 (Myc-DDK-tagged)-Human TBC1 (tre-2/USP6, BUB2, cdc16) domain family, member 1 (TBC1D1)



Reconstitution Protocol

USD 1,525.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol TBC1D1
Synonyms TBC; TBC1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC207980 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAACCAATAACATTCACAGCAAGGAAACATCTGCTTTCTAACGAGGTCTCGGTGGATTTTGGCCTGC
AGCTGGTGGGCTCCCTGCCTGTGCATTCCCTGACCACCATGCCCATGCTGCCCTGGGTTGTGGCTGAGGT
GCGAAGACTCAGCAGGCAGTCCACCAGAAAGGAACCTGTAACCAAGCAAGTCCGGCTTTGCGTTTCACCC
TCTGGACTGAGATGTGAACCTGAGCCAGGGAGAAGTCAACAGTGGGATCCCCTGATCTATTCCAGCATCT
TTGAGTGCAAGCCTCAGCGTGTTCACAAACTGATTCACAACAGTCATGACCCAAGTTACTTTGCTTGTCT
GATTAAGGAAGACGCTGTCCACCGGCAGAGTATCTGCTATGTGTTCAAAGCCGATGATCAAACAAAAGTG
CCTGAGATCATCAGCTCCATCCGTCAGGCGGGGAAGATCGCCCGGCAGGAGGAGCTGCACTGCCCGTCCG
AGTTCGACGACACGTTTTCCAAGAAGTTCGAGGTGCTCTTCTGCGGCCGCGTGACGGTGGCGCACAAGAA
GGCTCCGCCGGCCCTGATCGACGAGTGCATCGAGAAGTTCAATCACGTCAGCGGCAGCCGGGGGTCCGAG
AGCCCCCGCCCCAACCCGCCCCATGCCGCGCCCACAGGGAGCCAGGAGCCTGTGCGCAGGCCCATGCGCA
AGTCCTTCTCCCAGCCCGGCCTGCGCTCGCTGGCCTTTAGGAAGGAGCTGCAGGATGGGGGCCTCCGAAG
CAGCGGCTTCTTCAGCTCCTTCGAGGAGAGCGACATTGAGAACCACCTCATTAGCGGACACAATATTGTG
CAGCCCACAGATATCGAGGAAAATCGAACTATGCTCTTCACGATTGGCCAGTCTGAAGTTTACCTCATCA
GTCCTGACACCAAAAAAATAGCATTGGAGAAAAATTTTAAGGAGATATCCTTTTGCTCTCAGGGCATCAG
ACACGTGGACCACTTTGGGTTTATCTGTCGGGAGTCTTCCGGAGGTGGCGGCTTTCATTTTGTCTGTTAC
GTGTTTCAGTGCACAAATGAGGCTCTGGTTGATGAAATTATGATGACCCTGAAACAGGCCTTCACGGTGG
CCGCAGTGCAGCAGACAGCTAAGGCGCCAGCCCAGCTGTGTGAGGGCTGCCCCCTGCAAAGCCTGCACAA
GCTCTGTGAGAGGATAGAGGGAATGAATTCTTCCAAAACAAAACTAGAACTGCAAAAGCACCTGACGACA
TTAACAAATCAGGAGCAGGCGACTATTTTTGAAGAGGTTCAGAAATTGAGACCGAGAAATGAGCAGCGAG
AGAATGAATTGATTATTTCTTTTCTGAGATGTTTATATGAAGAGAAACAGAAAGAACACATCCATATTGG
GGAGATGAAGCAGACATCGCAGATGGCAGCAGAGAATATTGGAAGTGAATTACCACCCAGTGCCACTCGA
TTTAGGCTAGATATGCTGAAAAACAAAGCAAAGAGATCTTTAACAGAGTCTTTAGAAAGTATTTTGTCCC
GGGGTAATAAAGCCAGAGGCCTGCAGGAACACTCCATCAGTGTGGATCTGGATAGCTCCCTGTCTAGTAC
ATTAAGTAACACCAGCAAAGAGCCATCTGTGTGTGAAAAGGAGGCCTTGCCCATCTCTGAGAGCTCCTTT
AAGCTCCTCGGCTCCTCGGAGGACCTGTCCAGTGACTCGGAGAGTCATCTCCCAGAAGAGCCAGCTCCGC
TGTCGCCCCAGCAGGCCTTCAGGAGGCGAGCAAACACCCTGAGTCACTTCCCCATCGAATGCCAGGAACC
TCCACAACCTGCCCGGGGGTCCCCGGGGGTTTCGCAAAGGAAACTTATGAGGTATCACTCAGTGAGCACA
GAGACGCCTCATGAACGAAAGGACTTTGAATCCAAAGCAAACCATCTTGGTGATTCTGGTGGGACTCCTG
TGAAGACCCGGAGGCATTCCTGGAGGCAGCAGATATTCCTCCGAGTAGCCACCCCGCAGAAGGCGTGCGA
TTCTTCCAGCAGATATGAAGATTATTCAGAGCTGGGAGAGCTTCCCCCACGATCTCCTTTAGAACCAGTT
TGTGAAGATGGGCCCTTTGGCCCCCCACCAGAGGAAAAGAAAAGGACATCTCGTGAGCTCCGAGAGCTGT
GGCAAAAGGCTATTCTTAAACAGATACTGCTGCTTAGAATGGAGAAGGAAAATCAGAAGCTCCAAGCCTC
TGAAAATGATTTGCTGAACAAGCGCCTGAAGCTCGATTATGAAGAAATTACTCCCTGTCTTAAAGAAGTA
ACTACAGTGTGGGAAAAGATGCTTAGCACTCCAGGAAGATCAAAAATTAAGTTTGACATGGAAAAAATGC
ACTCGGCTGTTGGGCAAGGTGTGCCACGTCATCACCGAGGTGAAATCTGGAAATTTCTAGCTGAGCAATT
CCACCTTAAACACCAGTTTCCCAGCAAACAGCAGCCAAAGGATGTGCCATACAAAGAACTCTTAAAGCAG
CTGACTTCCCAGCAGCATGCGATTCTTATTGACCTTGGGCGAACCTTTCCTACACACCCATACTTCTCTG
CCCAGCTTGGAGCAGGACAGCTATCGCTTTACAACATTTTGAAGGCCTACTCACTTCTAGACCAGGAAGT
GGGATATTGCCAAGGTCTCAGCTTTGTAGCAGGCATTTTGCTTCTTCATATGAGTGAGGAAGAGGCGTTT
AAAATGCTCAAGTTTCTGATGTTTGACATGGGGCTGCGGAAACAGTATCGGCCAGACATGATTATTTTAC
AGATCCAGATGTACCAGCTCTCGAGGTTGCTTCATGATTACCACAGAGACCTCTACAATCACCTGGAGGA
GCACGAGATCGGCCCCAGCCTCTACGCTGCCCCCTGGTTCCTCACCATGTTTGCCTCACAGTTCCCGCTG
GGATTCGTAGCCAGAGTCTTTGATATGATTTTTCTTCAGGGAACAGAGGTCATATTTAAAGTGGCTTTAA
GTCTGTTGGGAAGCCATAAGCCCTTGATTCTGCAGCATGAAAACCTAGAAACCATAGTTGACTTTATAAA
AAGCACGCTACCCAACCTTGGCTTGGTACAGATGGAAAAGACCATCAATCAGGTATTTGAAATGGACATC
GCTAAACAGTTACAAGCTTATGAAGTTGAGTACCACGTCCTTCAAGAAGAACTTATCGATTCCTCTCCTC
TCAGTGACAACCAAAGAATGGATAAATTAGAGAAAACCAACAGCAGCTTACGCAAACAGAACCTTGACCT
CCTTGAACAGTTGCAGGTGGCAAATGGTAGGATCCAAAGCCTTGAGGCCACCATTGAGAAGCTCCTGAGC
AGTGAGAGCAAGCTGAAGCAGGCCATGCTTACCTTAGAACTGGAGCGGTCGGCCCTGCTGCAGACGGTGG
AGGAGCTGCGGCGGCGGAGCGCAGAGCCCAGCGACCGGGAGCCTGAGTGCACGCAGCCCGAGCCCACGGG
CGAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC207980 protein sequence
Red=Cloning site Green=Tags(s)

MEPITFTARKHLLSNEVSVDFGLQLVGSLPVHSLTTMPMLPWVVAEVRRLSRQSTRKEPVTKQVRLCVSP
SGLRCEPEPGRSQQWDPLIYSSIFECKPQRVHKLIHNSHDPSYFACLIKEDAVHRQSICYVFKADDQTKV
PEIISSIRQAGKIARQEELHCPSEFDDTFSKKFEVLFCGRVTVAHKKAPPALIDECIEKFNHVSGSRGSE
SPRPNPPHAAPTGSQEPVRRPMRKSFSQPGLRSLAFRKELQDGGLRSSGFFSSFEESDIENHLISGHNIV
QPTDIEENRTMLFTIGQSEVYLISPDTKKIALEKNFKEISFCSQGIRHVDHFGFICRESSGGGGFHFVCY
VFQCTNEALVDEIMMTLKQAFTVAAVQQTAKAPAQLCEGCPLQSLHKLCERIEGMNSSKTKLELQKHLTT
LTNQEQATIFEEVQKLRPRNEQRENELIISFLRCLYEEKQKEHIHIGEMKQTSQMAAENIGSELPPSATR
FRLDMLKNKAKRSLTESLESILSRGNKARGLQEHSISVDLDSSLSSTLSNTSKEPSVCEKEALPISESSF
KLLGSSEDLSSDSESHLPEEPAPLSPQQAFRRRANTLSHFPIECQEPPQPARGSPGVSQRKLMRYHSVST
ETPHERKDFESKANHLGDSGGTPVKTRRHSWRQQIFLRVATPQKACDSSSRYEDYSELGELPPRSPLEPV
CEDGPFGPPPEEKKRTSRELRELWQKAILKQILLLRMEKENQKLQASENDLLNKRLKLDYEEITPCLKEV
TTVWEKMLSTPGRSKIKFDMEKMHSAVGQGVPRHHRGEIWKFLAEQFHLKHQFPSKQQPKDVPYKELLKQ
LTSQQHAILIDLGRTFPTHPYFSAQLGAGQLSLYNILKAYSLLDQEVGYCQGLSFVAGILLLHMSEEEAF
KMLKFLMFDMGLRKQYRPDMIILQIQMYQLSRLLHDYHRDLYNHLEEHEIGPSLYAAPWFLTMFASQFPL
GFVARVFDMIFLQGTEVIFKVALSLLGSHKPLILQHENLETIVDFIKSTLPNLGLVQMEKTINQVFEMDI
AKQLQAYEVEYHVLQEELIDSSPLSDNQRMDKLEKTNSSLRKQNLDLLEQLQVANGRIQSLEATIEKLLS
SESKLKQAMLTLELERSALLQTVEELRRRSAEPSDREPECTQPEPTGD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_015173
ORF Size 3504 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_015173.4
RefSeq Size 5705 bp
RefSeq ORF 3507 bp
Locus ID 23216
UniProt ID Q86TI0
Cytogenetics 4p14
Domains TBC
Protein Families Druggable Genome
MW 133.1 kDa
Gene Summary TBC1D1 is the founding member of a family of proteins sharing a 180- to 200-amino acid TBC domain presumed to have a role in regulating cell growth and differentiation. These proteins share significant homology with TRE2 (USP6; MIM 604334), yeast Bub2, and CDC16 (MIM 603461) (White et al., 2000 [PubMed 10965142]).[supplied by OMIM, Mar 2008]
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