GALNT2 (NM_004481) Human Tagged ORF Clone

CAT#: RC207893

GALNT2 (Myc-DDK-tagged)-Human UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 2 (GalNAc-T2) (GALNT2)

Product Images

Circular map for RC207893

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  Western blot validation of overexpression lysate (Cat# LY417959) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207893 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified GALNT2 protein (Cat# TP307893). The protein was produced from HEK293T cells transfected with GALNT2 cDNA clone (Cat# RC207893) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GALNT2
• Synonyms: CDG2T; GalNAc-T2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207893 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGCGGCGCTCGCGGATGCTGCTCTGCTTCGCCTTCCTGTGGGTGCTGGGCATCGCCTACTACATGT
  ACTCGGGGGGCGGCTCTGCGCTGGCCGGGGGCGCGGGCGGCGGCGCCGGCAGGAAGGAGGACTGGAATGA
  AATTGACCCCATTAAAAAGAAAGACCTTCATCACAGCAATGGAGAAGAGAAAGCACAAAGCATGGAGACC
  CTCCCTCCAGGGAAAGTACGGTGGCCAGACTTTAACCAGGAAGCTTATGTTGGAGGGACGATGGTCCGCT
  CCGGGCAGGACCCTTACGCCCGCAACAAGTTCAACCAGGTGGAGAGTGATAAGCTTCGAATGGACAGAGC
  CATCCCTGACACCCGGCATGACCAGTGTCAGCGGAAGCAGTGGCGGGTGGATCTGCCGGCCACCAGCGTG
  GTGATCACGTTTCACAATGAAGCCAGGTCGGCCCTACTCAGGACCGTGGTCAGCGTGCTTAAGAAAAGCC
  CGCCCCATCTCATAAAAGAAATCATCTTGGTGGATGACTACAGCAATGATCCTGAGGACGGGGCTCTCTT
  GGGGAAAATTGAGAAAGTGCGAGTTCTTAGAAATGATCGACGAGAAGGCCTCATGCGCTCACGGGTTCGG
  GGGGCCGATGCTGCCCAAGCCAAGGTCCTGACCTTCCTGGACAGTCACTGCGAGTGTAATGAGCACTGGC
  TGGAGCCCCTCCTGGAAAGGGTGGCGGAGGACAGGACTCGGGTTGTGTCACCCATCATCGATGTCATTAA
  TATGGACAACTTTCAGTATGTGGGGGCATCTGCTGACTTGAAGGGCGGTTTTGATTGGAACTTGGTATTC
  AAGTGGGATTACATGACGCCTGAGCAGAGAAGGTCCCGGCAGGGGAACCCAGTCGCCCCTATAAAAACCC
  CCATGATTGCTGGTGGGCTGTTTGTGATGGATAAGTTCTATTTTGAAGAACTGGGGAAGTACGACATGAT
  GATGGATGTGTGGGGAGGAGAGAACCTAGAGATCTCGTTCCGCGTGTGGCAGTGTGGTGGCAGCCTGGAG
  ATCATCCCGTGCAGCCGTGTGGGACACGTGTTCCGGAAGCAGCACCCCTACACGTTCCCGGGTGGCAGTG
  GCACTGTCTTTGCCCGAAACACCCGCCGGGCAGCAGAGGTCTGGATGGATGAATACAAAAATTTCTATTA
  TGCAGCAGTGCCTTCTGCTAGAAACGTTCCTTATGGAAATATTCAGAGCAGATTGGAGCTTAGGAAGAAA
  CTCAGCTGCAAGCCTTTCAAATGGTACCTTGAAAATGTCTATCCAGAGTTAAGGGTTCCAGACCATCAGG
  ATATAGCTTTTGGGGCCTTGCAGCAGGGAACTAACTGCCTCGACACTTTGGGACACTTTGCTGATGGTGT
  GGTTGGAGTTTATGAATGTCACAATGCTGGGGGAAACCAGGAATGGGCCTTGACGAAGGAGAAGTCAGTG
  AAGCACATGGATTTGTGCCTTACTGTGGTGGACCGGGCACCGGGCTCTCTTATAAAGCTGCAGGGCTGCC
  GAGAAAATGACAGCAGACAGAAATGGGAACAGATCGAGGGCAACTCCAAGCTGAGGCACGTGGGCAGCAA
  CCTGTGCCTGGACAGTCGCACGGCCAAGAGCGGGGGCCTAAGCGTGGAGGTGTGTGGCCCGGCCCTTTCG
  CAGCAGTGGAAGTTCACGCTCAACCTGCAGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207893 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MRRRSRMLLCFAFLWVLGIAYYMYSGGGSALAGGAGGGAGRKEDWNEIDPIKKKDLHHSNGEEKAQSMET
  LPPGKVRWPDFNQEAYVGGTMVRSGQDPYARNKFNQVESDKLRMDRAIPDTRHDQCQRKQWRVDLPATSV
  VITFHNEARSALLRTVVSVLKKSPPHLIKEIILVDDYSNDPEDGALLGKIEKVRVLRNDRREGLMRSRVR
  GADAAQAKVLTFLDSHCECNEHWLEPLLERVAEDRTRVVSPIIDVINMDNFQYVGASADLKGGFDWNLVF
  KWDYMTPEQRRSRQGNPVAPIKTPMIAGGLFVMDKFYFEELGKYDMMMDVWGGENLEISFRVWQCGGSLE
  IIPCSRVGHVFRKQHPYTFPGGSGTVFARNTRRAAEVWMDEYKNFYYAAVPSARNVPYGNIQSRLELRKK
  LSCKPFKWYLENVYPELRVPDHQDIAFGALQQGTNCLDTLGHFADGVVGVYECHNAGGNQEWALTKEKSV
  KHMDLCLTVVDRAPGSLIKLQGCRENDSRQKWEQIEGNSKLRHVGSNLCLDSRTAKSGGLSVEVCGPALS
  QQWKFTLNLQQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004481
• ORF Size: 1713 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004481.5
• RefSeq Size: 4484 bp
• RefSeq ORF: 1716 bp
• Locus ID: 2590
• UniProt ID: Q10471
• Cytogenetics: 1q42.13
• Domains: RICIN, Glycos_transf_2
• Protein Families: Secreted Protein, Transmembrane
• Protein Pathways: Metabolic pathways, O-Glycan biosynthesis
• MW: 64.7 kDa
• Gene Summary: This gene encodes a member of the glycosyltransferase 2 protein family. Members of this family initiate mucin-type O-glycoslation of peptides in the Golgi apparatus. The encoded protein may be involved in O-linked glycosylation of the immunoglobulin A1 hinge region. This gene may influence triglyceride levels, and may be involved Type 2 diabetes, as well as several types of cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]