TP53TG5 (NM_014477) Human Tagged ORF Clone

CAT#: RC207754

TP53TG5 (Myc-DDK-tagged)-Human TP53 target 5 (TP53TG5)

Product Images

Circular map for RC207754

• 

  Western blot validation of overexpression lysate (Cat# LY415236) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207754 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TP53TG5
• Synonyms: C20orf10; CLG01
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207754 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTCCATCAGCAAAGAAGAGGCCCAAGAACAGCAGGGTTTCCAAGATGCAAGATGAGAAACTGCGGG
  ACGAGACAGAGCAGCCTGTGAGCAAAGTAATTGAGCGGAACCGTCTGAGAACGGTGTTAAAAAACTTGTC
  GCTCTTGAAGCTACTCAAGAGCTCAAACCGCCGGATCCAAGAACTGCATAAGCTGGCCAAAAGGTGTTGG
  CATTCACTGCTCAGTGTTCCAAAGATTCTCCGCATCTCCTCTGGGGAAAACAGTGCCTGCAATAAAACAA
  AACAAAATAATGAAGAGTTCCAGGAGATCGGGTGCTCCGAGAAGGAACTCAAGTCCAAGAAATTAGAGTC
  CACAGGGGACCCTAAGAAAAAAGAGTACAAGGAGTGGAAGTCCCAGGTGCAGTCAGGGATGAGGAACAAG
  GAGAAAACGTCATTGGCGGCAATGCCACGGAAAGAAAAGCATATAGAGCCTGAGGTTCCAAGGACATCGA
  GGGATGATAGCTTGAACCCTGGAGTCCAGGGGAGGCAACCACTCACCGAGGGCCCCCGAGTCATCTTCAT
  TAAGCCCTACCGCAATAGAACTCCCATGGGGCACATGAAGCAGCTGGATGTAGCCGACCAGTGGATCTGG
  TTTGAGGGGCTGCCCACACGAATCCACCTCCCGGCGCCCAGGGTGATGTGCAGATCCTCCACCCTGCGTT
  GGGTCAAGCGCCGCTGCACCCGCTTCTGCTCCGCATCACTTGAAATGCCTATGTGGCATCCATACAAGGT
  TGATGTGACCTGGACGAGAGCCAGAGGTGCGAGCAGAGGGTGGAGATCGCGCCATCAGCTGAAGGGGAGG
  AATGGGTGGCGAAATTCACGAGTCTACAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207754 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSPSAKKRPKNSRVSKMQDEKLRDETEQPVSKVIERNRLRTVLKNLSLLKLLKSSNRRIQELHKLAKRCW
  HSLLSVPKILRISSGENSACNKTKQNNEEFQEIGCSEKELKSKKLESTGDPKKKEYKEWKSQVQSGMRNK
  EKTSLAAMPRKEKHIEPEVPRTSRDDSLNPGVQGRQPLTEGPRVIFIKPYRNRTPMGHMKQLDVADQWIW
  FEGLPTRIHLPAPRVMCRSSTLRWVKRRCTRFCSASLEMPMWHPYKVDVTWTRARGASRGWRSRHQLKGR
  NGWRNSRVYK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014477
• ORF Size: 870 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014477.3
• RefSeq Size: 995 bp
• RefSeq ORF: 873 bp
• Locus ID: 27296
• UniProt ID: Q9Y2B4
• Cytogenetics: 20q13.12
• MW: 34 kDa
• Gene Summary: May play a significant role in p53/TP53-mediating signaling pathway.[UniProtKB/Swiss-Prot Function]