TTC30B (NM_152517) Human Tagged ORF Clone

CAT#: RC207665

TTC30B (Myc-DDK-tagged)-Human tetratricopeptide repeat domain 30B (TTC30B)

Product Images

Circular map for RC207665

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY TTC30B (Cat# RC207665, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TTC30B antibody (Cat# TA811993). Positive lysates LY407473 (100ug) and LC407473 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY407473) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207665 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TTC30B
• Synonyms: fleer; IFT70; IFT70B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207665 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGGCCTGAGCGGCGCGCAGATCCCCGACGGGGAGTTCACCGCGGTCGTGTACCGCCTCATCCGCA
  ATGCACGCTACGCCGAGGCGGTGCAGCTGCTGGGCGGAGAACTGCAGCGGAGCCCTAGGAGCCGCGCCGG
  CCTGTCGCTGCTAGGCTACTGCTACTACCGCCTGCAGGAGTTCGCGCTGGCGGCCGAGTGCTATGAGCAG
  CTGGGCCAGCTGCACCCGGAACTGGAGCAGTACCGCCTGTACCAGGCCCAGGCCCTATACAAGGCCTGCC
  TTTATGCGGAGGCCACCCGGGTCGCCTTCCTTCTCCTGGATAACCCCGCCTACCACAGCCGGGTCCTCCA
  CCTGCAAGCTGCTATCAAGTACAGCGAGGGCGATCTGCCAGGGTCCAGGAGCCTGGTAGAGCAGCTGCCG
  AGTAGGGAAGGGGGAGAGGAAAGTGGGGGCGAGAATGAGACCGATGGCCAGATCAACCTGGGTTGTTTGC
  TCTACAAGGAGGGACAGTATGAAGCTGCATGCTCCAAGTTTTTTGCCGCCCTGCAGGCCTCGGGCTACCA
  GCCTGACCTTTCCTACAACCTGGCTTTGGCCTATTACAGCAGCCGACAGTATGCTTCAGCACTGAAGCAT
  ATCGCTGAGATTATTGAGCGTGGCATCCGCCAGCACCCTGAGCTAGGTGTGGGCATGACCACTGAGGGCA
  TTGATGTTCGCAGTGTTGGCAACACCTTAGTCCTCCATCAGACTGCTCTGGTGGAAGCCTTCAACCTTAA
  GGCAGCTATAGAATACCAACTGAGAAACTATGAGGCAGCTCAAGAAGCCCTCACTGACATGCCACCCAGG
  GCAGAGGAAGAGTTGGACCCTGTGACCCTACACAACCAGGCACTAATGAACATGGATGCCAGGCCTACAG
  AAGGGTTTGAAAAGCTACAGTTTTTGCTCCAACAGAATCCCTTTCCTCCAGAGACTTTTGGCAACCTGTT
  GCTGCTCTACTGTAAATATGAGTATTTTGACCTGGCAGCAGATGTCCTGGCAGAAAATGCCCATTTGATT
  TATAAGTTCCTCACACCCTATCTCTATGACTTCTTGGACGCTGTGATCACTTGCCAGACAGCTCCTGAAG
  AGGCTTTCATTAAGCTTGATGGGCTAGCAGGGATGCTGACTGAGGTCCTCCGGAAACTTACCATACAAGT
  ACAGGAAGCAAGACACAATAGAGATGATGAAGCTATCAAAAAGGCAGTGAATGAATATGATGAAACCATG
  GAGAAATACATTCCTGTGTTGATGGCTCAGGCAAAAATCTACTGGAATCTTGAAAATTATCCAATGGTGG
  AAAAGATCTTCCGCAAATCTGTGGAATTCTGTAACGACCATGATGTGTGGAAGTTGAATGTGGCTCATGT
  TCTGTTCATGCAGGAAAACAAATACAAAGAAGCCATTGGTTTCTATGAACCCATAGTCAAGAAGCATTAT
  GATAACATCCTGAATGTCAGTGCTATTGTACTGGCTAATCTCTGTGTTTCCTATATTATGACAAGTCAAA
  ATGAAGAAGCAGAGGAGTTGATGAGGAAGATTGAAAAGGAGGAAGAGCAGCTCTCTTATGATGACCCAGA
  TAAGAAAATGTACCATCTCTGCATTGTGAATTTGGTGATAGGAACTCTTTATTGTGCCAAAGGAAATTAT
  GACTTTGGTATTTCTCGAGTTATCAAAAGCTTGGAACCTTACAACAAAAAGCTGGGAACAGACACCTGGT
  ATTATGCCAAAAGATGCTTCCTGTCCTTGTTAGAAAACATGTCAAAACACACAATCATGCTTCGTGATAG
  TGTTATTCAAGAATGTGTCCAGTTTCTAGAACACTGTGAACTTCATGGCAGAAACATACCTGCTGTTATT
  GAACAACCCCTGGAAGAAGAAAGAATGCATGTTGGAAAGAATACAGTCACATATGAGTCTAGGCAGTTAA
  AAGCTTTGATTTATGAGATTATAGGATGGAATATA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207665 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAGLSGAQIPDGEFTAVVYRLIRNARYAEAVQLLGGELQRSPRSRAGLSLLGYCYYRLQEFALAAECYEQ
  LGQLHPELEQYRLYQAQALYKACLYAEATRVAFLLLDNPAYHSRVLHLQAAIKYSEGDLPGSRSLVEQLP
  SREGGEESGGENETDGQINLGCLLYKEGQYEAACSKFFAALQASGYQPDLSYNLALAYYSSRQYASALKH
  IAEIIERGIRQHPELGVGMTTEGIDVRSVGNTLVLHQTALVEAFNLKAAIEYQLRNYEAAQEALTDMPPR
  AEEELDPVTLHNQALMNMDARPTEGFEKLQFLLQQNPFPPETFGNLLLLYCKYEYFDLAADVLAENAHLI
  YKFLTPYLYDFLDAVITCQTAPEEAFIKLDGLAGMLTEVLRKLTIQVQEARHNRDDEAIKKAVNEYDETM
  EKYIPVLMAQAKIYWNLENYPMVEKIFRKSVEFCNDHDVWKLNVAHVLFMQENKYKEAIGFYEPIVKKHY
  DNILNVSAIVLANLCVSYIMTSQNEEAEELMRKIEKEEEQLSYDDPDKKMYHLCIVNLVIGTLYCAKGNY
  DFGISRVIKSLEPYNKKLGTDTWYYAKRCFLSLLENMSKHTIMLRDSVIQECVQFLEHCELHGRNIPAVI
  EQPLEEERMHVGKNTVTYESRQLKALIYEIIGWNI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152517
• ORF Size: 1995 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152517.2, NP_689730.2
• RefSeq Size: 2652 bp
• RefSeq ORF: 1998 bp
• Locus ID: 150737
• UniProt ID: Q8N4P2
• Cytogenetics: 2q31.2
• MW: 76.1 kDa
• Gene Summary: Required for polyglutamylation of axonemal tubulin. Plays a role in anterograde intraflagellar transport (IFT), the process by which cilia precursors are transported from the base of the cilium to the site of their incorporation at the tip.[UniProtKB/Swiss-Prot Function]