SRXN1 (NM_080725) Human Tagged ORF Clone

CAT#: RC207654

SRXN1 (Myc-DDK-tagged)-Human sulfiredoxin 1 (SRXN1)

Product Images

Circular map for RC207654

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  Western blot validation of overexpression lysate (Cat# LY409045) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207654 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SRXN1
• Synonyms: C20orf139; Npn3; SRX; SRX1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207654 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCTGCGTGCAGGAGGAACGCTGGGCAGGGCCGGCGCGGGTCGGGGGGCGCCCGAGGGGCCCGGGC
  CGAGCGGCGGCGCGCAGGGCGGCAGCATCCACTCGGGCCGCATCGCCGCGGTGCACAACGTGCCGCTGAG
  CGTGCTCATCCGGCCGCTGCCGTCCGTGTTGGACCCCGCCAAGGTGCAGAGCCTCGTGGACACGATCCGG
  GAGGACCCAGACAGCGTGCCCCCCATCGATGTCCTCTGGATCAAAGGGGCCCAGGGAGGTGACTACTTCT
  ACTCCTTTGGGGGCTGCCACCGCTACGCGGCCTACCAGCAACTGCAGCGAGAGACCATCCCCGCCAAGCT
  TGTCCAGTCCACTCTCTCAGACCTAAGGGTGTACCTGGGAGCATCCACACCAGACTTGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207654 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGLRAGGTLGRAGAGRGAPEGPGPSGGAQGGSIHSGRIAAVHNVPLSVLIRPLPSVLDPAKVQSLVDTIR
  EDPDSVPPIDVLWIKGAQGGDYFYSFGGCHRYAAYQQLQRETIPAKLVQSTLSDLRVYLGASTPDLQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_080725
• ORF Size: 411 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_080725.3
• RefSeq Size: 2704 bp
• RefSeq ORF: 414 bp
• Locus ID: 140809
• UniProt ID: Q9BYN0
• Cytogenetics: 20p13
• MW: 14.3 kDa
• Gene Summary: Contributes to oxidative stress resistance by reducing cysteine-sulfinic acid formed under exposure to oxidants in the peroxiredoxins PRDX1, PRDX2, PRDX3 and PRDX4. Does not act on PRDX5 or PRDX6. May catalyze the reduction in a multi-step process by acting both as a specific phosphotransferase and a thioltransferase.[UniProtKB/Swiss-Prot Function]