SRXN1 (NM_080725) Human Tagged ORF Clone

CAT#: RC207654

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SRXN1 (Myc-DDK-tagged)-Human sulfiredoxin 1 (SRXN1)



  "NM_080725" in other vectors (6)

Reconstitution Protocol

USD 225.00

USD 338.00


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Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol SRXN1
Synonyms C20orf139; Npn3; SRX; SRX1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC207654 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGCTGCGTGCAGGAGGAACGCTGGGCAGGGCCGGCGCGGGTCGGGGGGCGCCCGAGGGGCCCGGGC
CGAGCGGCGGCGCGCAGGGCGGCAGCATCCACTCGGGCCGCATCGCCGCGGTGCACAACGTGCCGCTGAG
CGTGCTCATCCGGCCGCTGCCGTCCGTGTTGGACCCCGCCAAGGTGCAGAGCCTCGTGGACACGATCCGG
GAGGACCCAGACAGCGTGCCCCCCATCGATGTCCTCTGGATCAAAGGGGCCCAGGGAGGTGACTACTTCT
ACTCCTTTGGGGGCTGCCACCGCTACGCGGCCTACCAGCAACTGCAGCGAGAGACCATCCCCGCCAAGCT
TGTCCAGTCCACTCTCTCAGACCTAAGGGTGTACCTGGGAGCATCCACACCAGACTTGCAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC207654 protein sequence
Red=Cloning site Green=Tags(s)

MGLRAGGTLGRAGAGRGAPEGPGPSGGAQGGSIHSGRIAAVHNVPLSVLIRPLPSVLDPAKVQSLVDTIR
EDPDSVPPIDVLWIKGAQGGDYFYSFGGCHRYAAYQQLQRETIPAKLVQSTLSDLRVYLGASTPDLQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_080725
ORF Size 411 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_080725.3
RefSeq Size 2704 bp
RefSeq ORF 414 bp
Locus ID 140809
UniProt ID Q9BYN0
Cytogenetics 20p13
MW 14.3 kDa
Gene Summary Contributes to oxidative stress resistance by reducing cysteine-sulfinic acid formed under exposure to oxidants in the peroxiredoxins PRDX1, PRDX2, PRDX3 and PRDX4. Does not act on PRDX5 or PRDX6. May catalyze the reduction in a multi-step process by acting both as a specific phosphotransferase and a thioltransferase.[UniProtKB/Swiss-Prot Function]

Other Versions

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