Host cell factor C2 (HCFC2) (NM_013320) Human Tagged ORF Clone

CAT#: RC207543

HCFC2 (Myc-DDK-tagged)-Human host cell factor C2 (HCFC2)

Product Images

Circular map for RC207543

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY HCFC2 (Cat# RC207543, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HCFC2(Cat# TA505235). Positive lysates LY415672 (100ug) and LC415672 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY415672) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207543 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Host cell factor C2
• Synonyms: HCF-2; HCF2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207543 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCTCCCAGCCTCCTCAACTGGAGGCGAGTTTCTTCCTTCACGGGGCCGGTCCCCCGCGCCCGGC
  ACGGACACCGAGCGGTGGCCATCCGGGAGCTGATGATCATCTTTGGAGGGGGAAATGAGGGCATCGCGGA
  TGAGCTGCACGTCTACAACACGGCTACGAATCAGTGGTTTCTGCCAGCTGTTAGAGGAGATATCCCTCCA
  GGCTGTGCTGCCCATGGATTTGTCTGTGATGGTACCAGAATATTAGTATTTGGGGGAATGGTTGAATATG
  GAAGATACAGCAATGAGTTATATGAGTTACAAGCAAGTCGTTGGTTATGGAAAAAAGTGAAACCCCATCC
  CCCTCCTTCTGGTTTACCTCCTTGTCCTCGGCTTGGACATAGCTTCTCTTTATATGGTAACAAATGCTAT
  TTGTTTGGTGGCCTGGCAAACGAAAGCGAAGATTCAAACAATAATGTTCCCAGATATTTAAATGATTTTT
  ATGAGTTGGAGCTACAGCATGGCTCTGGTGTTGTGGGTTGGAGCATTCCAGTGACTAAAGGGGTTGTGCC
  TTCTCCAAGAGAATCCCACACAGCTGTTATATATTGCAAAAAAGATTCTGGAAGTCCTAAAATGTATGTT
  TTTGGTGGAATGTGTGGTGCTCGCCTGGATGACCTATGGCAGCTTGACTTAGAAACTATGTCATGGTCAA
  AACCAGAAACTAAAGGGACAGTGCCACTTCCACGAAGCCTTCATACAGCCAGTGTTATAGGAAACAAGAT
  GTACATTTTTGGTGGATGGGTCCCACATAAGGGGGAAAATACTGAGACTTCACCTCATGATTGTGAATGG
  AGATGTACCAGTTCATTTTCTTACCTAAATCTGGATACAACAGAGTGGACCACCCTAGTATCAGATTCTC
  AGGAAGATAAAAAAAATTCAAGACCAAGACCAAGAGCTGGCCACTGTGCTGTTGCAATCGGCACTCGATT
  GTATTTTTGGAGTGGAAGAGATGGCTACAAAAAAGCACTGAATAGTCAAGTTTGCTGCAAGGATCTTTGG
  TATCTTGATACTGAGAAACCACCGGCACCATCTCAAGTACAGCTGATCAAAGCCACTACCAACTCCTTTC
  ATGTCAAGTGGGATGAAGTGTCTACAGTTGAGGGCTATCTTTTGCAGTTGAGTACAGACTTGCCATACCA
  AGCTGCATCATCAGATTCTTCAGCAGCACCAAATATGCAAGGAGTCAGGATGGACCCTCACAGACAAGGC
  AGTAATAACATCGTTCCTAACAGTATCAATGATACAATAAACAGCACAAAAACTGAACAGCCAGCCACAA
  AAGAAACTTCAATGAAAAACAAACCAGACTTTAAAGCACTGACGGATTCTAATGCCATTTTATATCCATC
  TTTGGCATCAAATGCTTCTAATCATAATAGTCATGTGGTGGATATGCTAAGGAAAAATGAAGGTCCTCAC
  ACTTCAGCAAATGTAGGTGTTCTAAGTAGTTGCCTGGATGTAAGAACAGTAATTCCTGAAACATCTGTAT
  CCAGTACTGTTTCCAGCACACAAACTATGGTAACCCAGCAGACCATTAAAACTGAATCATCCAGTACAAA
  TGGGGCAGTTGTTAAAGATGAAACTTCACTAACAACATTCAGTACCAAATCTGAAGTTGATGAAACATAT
  GCACTGCCTGCAACGAAGATCAGCCGTGTAGAGACACATGCTACAGCAACGCCGTTTTCTAAAGAGACTC
  CTTCAAATCCAGTGGCCACAGTGAAAGCGGGAGAACGACAATGGTGTGATGTGGGAATTTTTAAAAATAA
  TACAGCTTTGGTGAGCCAGTTTTATTTGCTGCCAAAAGGGAAGCAAAGCATCTCAAAGGTAGGAAATGCA
  GATGTACCTGACTACAGCTTGCTTAAGAAACAAGATCTTGTTCCAGGCACAGGATACAGATTCAGGGTTG
  CTGCAATCAATGGTTGTGGGATAGGTCCTTTCAGCAAAATCAGTGAATTTAAAACTTGTATTCCTGGTTT
  TCCTGGAGCTCCTTCTGCAGTCAGAATTTCAAAGAATGTTGAAGGTATCCACCTTTCCTGGGAACCTCCA
  ACCTCACCTTCTGGAAATATTTTGGAATATTCAGCCTACTTGGCTATCCGCACAGCACAGATACAAGATA
  ATCCAAGTCAACTTGTGTTCATGAGGATTTATTGTGGTCTTAAGACATCATGTATAGTAACTGCTGGGCA
  ACTTGCAAATGCACATATTGATTATACATCCAGGCCTGCCATTGTGTTCAGGATATCAGCAAAGAATGAA
  AAGGGATATGGACCAGCTACACAAGTTCGGTGGCTTCAAGGTAACAATAAGAAAGCACCTTTAAAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207543 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAPSLLNWRRVSSFTGPVPRARHGHRAVAIRELMIIFGGGNEGIADELHVYNTATNQWFLPAVRGDIPP
  GCAAHGFVCDGTRILVFGGMVEYGRYSNELYELQASRWLWKKVKPHPPPSGLPPCPRLGHSFSLYGNKCY
  LFGGLANESEDSNNNVPRYLNDFYELELQHGSGVVGWSIPVTKGVVPSPRESHTAVIYCKKDSGSPKMYV
  FGGMCGARLDDLWQLDLETMSWSKPETKGTVPLPRSLHTASVIGNKMYIFGGWVPHKGENTETSPHDCEW
  RCTSSFSYLNLDTTEWTTLVSDSQEDKKNSRPRPRAGHCAVAIGTRLYFWSGRDGYKKALNSQVCCKDLW
  YLDTEKPPAPSQVQLIKATTNSFHVKWDEVSTVEGYLLQLSTDLPYQAASSDSSAAPNMQGVRMDPHRQG
  SNNIVPNSINDTINSTKTEQPATKETSMKNKPDFKALTDSNAILYPSLASNASNHNSHVVDMLRKNEGPH
  TSANVGVLSSCLDVRTVIPETSVSSTVSSTQTMVTQQTIKTESSSTNGAVVKDETSLTTFSTKSEVDETY
  ALPATKISRVETHATATPFSKETPSNPVATVKAGERQWCDVGIFKNNTALVSQFYLLPKGKQSISKVGNA
  DVPDYSLLKKQDLVPGTGYRFRVAAINGCGIGPFSKISEFKTCIPGFPGAPSAVRISKNVEGIHLSWEPP
  TSPSGNILEYSAYLAIRTAQIQDNPSQLVFMRIYCGLKTSCIVTAGQLANAHIDYTSRPAIVFRISAKNE
  KGYGPATQVRWLQGNNKKAPLN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013320
• ORF Size: 2376 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013320.3
• RefSeq Size: 5743 bp
• RefSeq ORF: 2379 bp
• Locus ID: 29915
• UniProt ID: Q9Y5Z7
• Cytogenetics: 12q23.3
• Domains: FN3, Kelch
• Protein Families: Transcription Factors
• MW: 86.8 kDa
• Gene Summary: This gene encodes one of two proteins which interact with VP16, a herpes simplex virus protein that initiates virus infection. Both the encoded protein and the original Herpes host cell factor interact with VP16 through a beta-propeller domain. The original Herpes host cell factor, however, is effective at initiating viral infection while the encoded protein is not. Transcripts of varying length due to alternative polyadenylation signals have been described. [provided by RefSeq, Jul 2008]