CaMKI gamma (CAMK1G) (NM_020439) Human Tagged ORF Clone

CAT#: RC207444

CAMK1G (Myc-DDK-tagged)-Human calcium/calmodulin-dependent protein kinase IG (CAMK1G)

Product Images

Circular map for RC207444

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  Western blot validation of overexpression lysate (Cat# LY402790) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207444 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified CAMK1G protein (Cat# TP307444). The protein was produced from HEK293T cells transfected with CAMK1G cDNA clone (Cat# RC207444) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CaMKI gamma
• Synonyms: CLICK3; CLICKIII; dJ272L16.1; VWS1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207444 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTCGAAAGGAAGAAGATGACTGCAGTTCCTGGAAGAAACAGACCACCAACATCCGGAAAACCTTCA
  TTTTTATGGAAGTGCTGGGATCAGGAGCTTTCTCAGAAGTTTTCCTGGTGAAGCAAAGACTGACTGGGAA
  GCTCTTTGCTCTGAAGTGCATCAAGAAGTCACCTGCCTTCCGGGACAGCAGCCTGGAGAATGAGATTGCT
  GTGTTGAAAAAGATCAAGCATGAAAACATTGTGACCCTGGAGGACATCTATGAGAGCACCACCCACTACT
  ACCTGGTCATGCAGCTTGTTTCTGGTGGGGAGCTCTTTGACCGGATCCTGGAGCGGGGTGTCTACACAGA
  GAAGGATGCCAGTCTGGTGATCCAGCAGGTCTTGTCGGCAGTGAAATACCTACATGAGAATGGCATCGTC
  CACAGAGACTTAAAGCCCGAAAACCTGCTTTACCTTACCCCTGAAGAGAACTCTAAGATCATGATCACTG
  ACTTTGGTCTGTCCAAGATGGAACAGAATGGCATCATGTCCACTGCCTGTGGGACCCCAGGCTACGTGGC
  TCCAGAAGTGCTGGCCCAGAAACCCTACAGCAAGGCTGTGGATTGCTGGTCCATCGGCGTCATCACCTAC
  ATATTGCTCTGTGGATACCCCCCGTTCTATGAAGAAACGGAGTCTAAGCTTTTCGAGAAGATCAAGGAGG
  GCTACTATGAGTTTGAGTCTCCATTCTGGGATGACATTTCTGAGTCAGCCAAGGACTTTATTTGCCACTT
  GCTTGAGAAGGATCCGAACGAGCGGTACACCTGTGAGAAGGCCTTGAGTCATCCCTGGATTGACGGAAAC
  ACAGCCCTCCACCGGGACATCTACCCATCAGTCAGCCTCCAGATCCAGAAGAACTTTGCTAAGAGCAAGT
  GGAGGCAAGCCTTCAACGCAGCAGCTGTGGTGCACCACATGAGGAAGCTACACATGAACCTGCACAGCCC
  GGGCGTCCGCCCAGAGGTGGAGAACAGGCCGCCTGAAACTCAAGCCTCAGAAACCTCTAGACCCAGCTCC
  CCTGAGATCACCATCACCGAGGCACCTGTCCTGGACCACAGTGTAGCACTCCCTGCCCTGACCCAATTAC
  CCTGCCAGCATGGCCGCCGGCCCACTGCCCCTGGTGGCAGGTCCCTCAACTGCCTGGTCAATGGCTCCCT
  CCACATCAGCAGCAGCCTGGTGCCCATGCATCAGGGGTCCCTGGCCGCCGGGCCCTGTGGCTGCTGCTCC
  AGCTGCCTGAACATTGGGAGCAAAGGAAAGTCCTCCTACTGCTCTGAGCCCACACTCCTCAAAAAGGCCA
  ACAAAAAACAGAACTTCAAGTCGGAGGTCATGGTACCAGTTAAAGCCAGTGGCAGCTCCCACTGCCGGGC
  AGGGCAGACTGGAGTCTGTCTCATTATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207444 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGRKEEDDCSSWKKQTTNIRKTFIFMEVLGSGAFSEVFLVKQRLTGKLFALKCIKKSPAFRDSSLENEIA
  VLKKIKHENIVTLEDIYESTTHYYLVMQLVSGGELFDRILERGVYTEKDASLVIQQVLSAVKYLHENGIV
  HRDLKPENLLYLTPEENSKIMITDFGLSKMEQNGIMSTACGTPGYVAPEVLAQKPYSKAVDCWSIGVITY
  ILLCGYPPFYEETESKLFEKIKEGYYEFESPFWDDISESAKDFICHLLEKDPNERYTCEKALSHPWIDGN
  TALHRDIYPSVSLQIQKNFAKSKWRQAFNAAAVVHHMRKLHMNLHSPGVRPEVENRPPETQASETSRPSS
  PEITITEAPVLDHSVALPALTQLPCQHGRRPTAPGGRSLNCLVNGSLHISSSLVPMHQGSLAAGPCGCCS
  SCLNIGSKGKSSYCSEPTLLKKANKKQNFKSEVMVPVKASGSSHCRAGQTGVCLIM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020439
• ORF Size: 1428 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020439.3
• RefSeq Size: 2507 bp
• RefSeq ORF: 1431 bp
• Locus ID: 57172
• UniProt ID: Q96NX5
• Cytogenetics: 1q32.2
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• MW: 53.1 kDa
• Gene Summary: This gene encodes a protein similar to calcium/calmodulin dependent protein kinase, however, its exact function is not known. [provided by RefSeq, Jul 2008]