TRIM22 (NM_006074) Human Tagged ORF Clone

CAT#: RC207431

TRIM22 (Myc-DDK-tagged)-Human tripartite motif containing 22 (TRIM22), transcript variant 1

Product Images

Circular map for RC207431

• 

  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY TRIM22 (Cat# RC207431, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIM22(Cat# TA505281). Positive lysates LY401829 (100ug) and LC401829 (20ug) can be purchased separately from OriGene.

• 

  Western blot validation of overexpression lysate (Cat# LY401829) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207431 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TRIM22
• Synonyms: GPSTAF50; RNF94; STAF50
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207431 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATTTCTCAGTAAAGGTAGACATAGAGAAGGAGGTGACCTGCCCCATCTGCCTGGAGCTCCTGACAG
  AACCTCTGAGCCTAGATTGTGGCCACAGCTTCTGCCAAGCCTGCATCACTGCAAAGATCAAGGAGTCAGT
  GATCATCTCAAGAGGGGAAAGCAGCTGTCCTGTGTGTCAGACCAGATTCCAGCCTGGGAACCTCCGACCT
  AATCGGCATCTGGCCAACATAGTTGAGAGAGTCAAAGAGGTCAAGATGAGCCCACAGGAGGGGCAGAAGA
  GAGATGTCTGTGAGCACCATGGAAAAAAACTCCAGATCTTCTGTAAGGAGGATGGAAAAGTCATTTGCTG
  GGTTTGTGAACTGTCTCAGGAACACCAAGGTCACCAAACATTCCGCATAAACGAGGTGGTCAAGGAATGT
  CAGGAAAAGCTGCAGGTAGCCCTGCAGAGGCTGATAAAGGAGGATCAAGAGGCTGAGAAGCTGGAAGATG
  ACATCAGACAAGAGAGAACCGCCTGGAAGAATTATATCCAGATCGAGAGACAGAAGATTCTGAAAGGGTT
  CAATGAAATGAGAGTCATCTTGGACAATGAGGAGCAGAGAGAGCTGCAAAAGCTGGAGGAAGGTGAGGTG
  AATGTGCTGGACAACCTGGCAGCAGCTACAGACCAGCTGGTCCAGCAGAGGCAGGATGCCAGCACGCTCA
  TCTCAGATCTCCAGCGGAGGTTGAGGGGATCGTCAGTAGAGATGCTGCAGGATGTGATTGACGTCATGAA
  AAGGAGTGAAAGCTGGACATTGAAGAAGCCAAAATCTGTTTCCAAGAAACTAAAGAGTGTATTCCGAGTA
  CCAGATCTGAGTGGGATGCTGCAAGTTCTTAAAGAGCTGACAGATGTCCAGTACTACTGGGTGGACGTGA
  TGCTGAATCCAGGCAGTGCCACTTCGAATGTTGCTATTTCTGTGGATCAGAGACAAGTGAAAACTGTACG
  CACCTGCACATTTAAGAATTCAAATCCATGTGATTTTTCTGCTTTTGGTGTCTTCGGCTGCCAATATTTC
  TCTTCGGGGAAATATTACTGGGAAGTAGATGTGTCTGGAAAGATTGCCTGGATCCTGGGCGTACACAGTA
  AAATAAGTAGTCTGAATAAAAGGAAGAGCTCTGGGTTTGCTTTTGATCCAAGTGTAAATTATTCAAAAGT
  TTACTCCAGATATAGACCTCAATATGGCTACTGGGTTATAGGATTACAGAATACATGTGAATATAATGCT
  TTTGAGGACTCCTCCTCTTCTGATCCCAAGGTTTTGACTCTCTTTATGGCTGTGCCTCCCTGTCGTATTG
  GGGTTTTCCTAGACTATGAGGCAGGCATTGTCTCATTTTTCAATGTCACAAACCACGGAGCACTCATCTA
  CAAGTTCTCTGGATGTCGCTTTTCTCGACCTGCTTATCCGTATTTCAATCCTTGGAACTGCCTAGTCCCC
  ATGACTGTGTGCCCACCGAGCTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207431 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDFSVKVDIEKEVTCPICLELLTEPLSLDCGHSFCQACITAKIKESVIISRGESSCPVCQTRFQPGNLRP
  NRHLANIVERVKEVKMSPQEGQKRDVCEHHGKKLQIFCKEDGKVICWVCELSQEHQGHQTFRINEVVKEC
  QEKLQVALQRLIKEDQEAEKLEDDIRQERTAWKNYIQIERQKILKGFNEMRVILDNEEQRELQKLEEGEV
  NVLDNLAAATDQLVQQRQDASTLISDLQRRLRGSSVEMLQDVIDVMKRSESWTLKKPKSVSKKLKSVFRV
  PDLSGMLQVLKELTDVQYYWVDVMLNPGSATSNVAISVDQRQVKTVRTCTFKNSNPCDFSAFGVFGCQYF
  SSGKYYWEVDVSGKIAWILGVHSKISSLNKRKSSGFAFDPSVNYSKVYSRYRPQYGYWVIGLQNTCEYNA
  FEDSSSSDPKVLTLFMAVPPCRIGVFLDYEAGIVSFFNVTNHGALIYKFSGCRFSRPAYPYFNPWNCLVP
  MTVCPPSS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006074
• ORF Size: 1494 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006074.5
• RefSeq Size: 3111 bp
• RefSeq ORF: 1497 bp
• Locus ID: 10346
• UniProt ID: Q8IYM9
• Cytogenetics: 11p15.4
• Domains: zf-B_box, RING
• Protein Families: Druggable Genome, Transcription Factors
• MW: 56.9 kDa
• Gene Summary: The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to the cytoplasm and its expression is induced by interferon. The protein down-regulates transcription from the HIV-1 LTR promoter region, suggesting that function of this protein may be to mediate interferon's antiviral effects. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2010]