CCDC65 (NM_033124) Human Tagged ORF Clone

CAT#: RC207167

CCDC65 (Myc-DDK-tagged)-Human coiled-coil domain containing 65 (CCDC65)

Product Images

Circular map for RC207167

• 

  Western blot validation of overexpression lysate (Cat# LY409729) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207167 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CCDC65
• Synonyms: CFAP250; DRC2; FAP250; NYD-SP28
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207167 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTAAGAAAGAAAAAATGGCCAAGACGCCCCTGTCCGATGAGAAGCAGCTGCTTCTGTTTCAGCAGA
  AGTTGCTGGCAGAGGAGGAGATGGCCAAAAAGAAGGAGAGGCTCCTCAGCCAGTTCTTGAAGGACAAGCT
  GGCCAAGGAGGAACACAACAGTGCTCTGAACCTTAATAAGATTAACACACAGTGGAGAACTGTCCTTCGG
  GAAGTCAAGACCAGAGAACTTCATAAGGACATTGAGATCCTCAGCCAAACATTTGAACGAGTGGTGGACT
  GTAAGGACAATGTCATCAAGTCTTTAGCTAAAGACCTGTCCGAAGCCGAGGAGCAGTACGCCCATGCCCT
  GCGCAGCCACTTGCACAATGTTGACCAGCTCTTGGCCCTGCAGAGGCACCGGCTCAGTCTCCTGGAGGAA
  AGTTACAACATGGAGCTGGAAGCCCTAACCAAGGAGTTTGAGACAGAAAGGAAGACAATTATTGACCAAC
  ATGAGAAAGAGATTCACTATCTGCAAGATATCTTCATGGCCATGGAGCAGAACTATATAGATTCTGAGTA
  TGAAAGCAAGCTGGAGTTCCAGAGCATGTGGAATGATCTCAAAAACATGAATTTAGAAGAGAAGCACTTT
  CTAAGACTGCACCTGGAGAACAGAGTAGAAGATCTGTGGAGAAAGTTCCAGGATGTACTCAAGAATTACA
  CTGATGCCACAGAGGATCGAAAGGCTGCCTTTGAGACCCTGCAGGTGAAGGATGAGAAGAGCTCCAAAGA
  GATTGAAGTACAGATGAAAAAAATACAGAAACTACAGGATGCCATAACTATTTCAAAAGGCAAGATCATG
  ATACACAGCCGTGAGAGTGAAGATGAGAACCGGTATATCCGTAATGACAAGGAATTGGTCCTTGTACAAC
  TGCGAAAACTTAAGGCCCAAAGAACTCAGGCCCGGGCAGCATCCCAGAAGAACTTAGTCAGACTCACCCT
  GGAAAGTAATGCCACCCTCAAGGCCCTGAGAAAGATTGTTGATAAGGGTGAAAAGATCCTTAAACTTGCT
  GAAATATGTAGGAAATTTGAAACCGAAGAAGAAAAAGTGCTGCCTTTTTATTCATCAGTATTGACTCCTA
  AGGAGCAGGAGGGGATTCAGAAGAATAATCTAGAAGAGCTTACTGAGGAGCTCACCAAGGTGATGGTGGA
  CTACATAGGAATGGAGAATTTCTGGAAAAGGTACAACAAAGTGAAACTGGAGCAACTGAGCCTCCAACAT
  AGACGAGCCCAGCTGCTAGATATCAATGGGAAGCTGCGGGAGATGCTGAAGCAGTACTTGGATGGCATCT
  CAGTGAGTGACGAAGTGCTGAGCCAGCTCAACCCACTCTTTATAGTCAACTATCAAAGCAACTTACTCCA
  GCCCTTGTCCATACGTATAGCCCATCCAGGTGATAAACAACATCCAACCACT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207167 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPKKEKMAKTPLSDEKQLLLFQQKLLAEEEMAKKKERLLSQFLKDKLAKEEHNSALNLNKINTQWRTVLR
  EVKTRELHKDIEILSQTFERVVDCKDNVIKSLAKDLSEAEEQYAHALRSHLHNVDQLLALQRHRLSLLEE
  SYNMELEALTKEFETERKTIIDQHEKEIHYLQDIFMAMEQNYIDSEYESKLEFQSMWNDLKNMNLEEKHF
  LRLHLENRVEDLWRKFQDVLKNYTDATEDRKAAFETLQVKDEKSSKEIEVQMKKIQKLQDAITISKGKIM
  IHSRESEDENRYIRNDKELVLVQLRKLKAQRTQARAASQKNLVRLTLESNATLKALRKIVDKGEKILKLA
  EICRKFETEEEKVLPFYSSVLTPKEQEGIQKNNLEELTEELTKVMVDYIGMENFWKRYNKVKLEQLSLQH
  RRAQLLDINGKLREMLKQYLDGISVSDEVLSQLNPLFIVNYQSNLLQPLSIRIAHPGDKQHPTT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_033124
• ORF Size: 1452 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033124.5
• RefSeq Size: 1832 bp
• RefSeq ORF: 1455 bp
• Locus ID: 85478
• UniProt ID: Q8IXS2
• Cytogenetics: 12q13.12
• MW: 57.3 kDa
• Gene Summary: This gene encodes a sperm tail protein that is highly expressed in adult testis, spermatocytes and spermatids. The protein plays a critical role in the assembly of the nexin-dynein regulatory complex. Mutations in this gene result in primary ciliary dyskinesia. [provided by RefSeq, Nov 2013]