FAM89B (NM_152832) Human Tagged ORF Clone
CAT#: RC207134
FAM89B (Myc-DDK-tagged)-Human family with sequence similarity 89, member B (FAM89B), transcript variant 2
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_152832" in other vectors (5)
USD 198.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | FAM89B |
Synonyms | LRAP25; MTVR; MTVR1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC207134 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACGGGCTGCCCTCGGCAGAGGCGCCGGGCGGGGCGGGCTGCGCTTTGGCCGGGCTCCCACCGCTGC CGCGCGGCCTCAGCGGCCTCCTTAATGCGAGCGGGGGCTCGTGGCGGGAGCTGGAGCGCGTCTACAGCCA GCGCAGCCGCATCCACGACGAGCTGAGCCGCGCCGCCCGCGCCCCGGACGGGCCCCGCCACGCCGCCGGC GCCGCCAACGCGGGACCCGCAGCCGGCCCGCGTCGTCCTGTCAACCTCGACTCAGCGCTGGCCGCGCTGC GCAAGGAGATGCTGTGGGGCCTGTACGAGTCAATCCAGGACTACAAACACCTGTGCCAAGACCTGAGCTT CTGCCAGGACCTGTCATCCTCCCTCCATTCGGACAGCTCCTACCCACCGGATGCGGGCCTGTCTGACGAC GAGGAGCCTCCCGATGCCAGCCTGCCTCCTGACCCGCCACCCCTTACTGTGCCCCAGACGCACAATGCCC GTGACCAGTGGCTGCAGGATGCCTTCCACATCAGCCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC207134 protein sequence
Red=Cloning site Green=Tags(s) MNGLPSAEAPGGAGCALAGLPPLPRGLSGLLNASGGSWRELERVYSQRSRIHDELSRAARAPDGPRHAAG AANAGPAAGPRRPVNLDSALAALRKEMLWGLYESIQDYKHLCQDLSFCQDLSSSLHSDSSYPPDAGLSDD EEPPDASLPPDPPPLTVPQTHNARDQWLQDAFHISL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_152832 |
ORF Size | 528 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_152832.3 |
RefSeq Size | 1428 bp |
RefSeq ORF | 531 bp |
Locus ID | 23625 |
UniProt ID | Q8N5H3 |
Cytogenetics | 11q13.1 |
Protein Families | Druggable Genome |
MW | 18.7 kDa |
Gene Summary | Negatively regulates TGF-beta-induced signaling; in cooperation with SKI prevents the translocation of SMAD2 from the nucleus to the cytoplasm in response to TGF-beta. Acts as an adapter that mediates the specific recognition of LIMK1 by CDC42BPA and CDC42BPB in the lamellipodia. LRAP25-mediated CDC42BPA/CDC42BPB targeting to LIMK1 and the lamellipodium results in LIMK1 activation and the subsequent phosphorylation of CFL1 which is important for lamellipodial F-actin regulation.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC207134L3 | Lenti-ORF clone of FAM89B (Myc-DDK-tagged)-Human family with sequence similarity 89, member B (FAM89B), transcript variant 2 |
USD 600.00 |
|
RC207134L4 | Lenti-ORF clone of FAM89B (mGFP-tagged)-Human family with sequence similarity 89, member B (FAM89B), transcript variant 2 |
USD 600.00 |
|
RG207134 | FAM89B (tGFP-tagged) - Human family with sequence similarity 89, member B (FAM89B), transcript variant 2 |
USD 500.00 |
|
SC108157 | FAM89B (untagged)-Human family with sequence similarity 89, member B (FAM89B), transcript variant 2 |
USD 300.00 |
|
SC322584 | FAM89B (untagged)-Human family with sequence similarity 89, member B (FAM89B), transcript variant 2 |
USD 300.00 |
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